EBK BIOLOGY
EBK BIOLOGY
5th Edition
ISBN: 9780133954425
Author: Maier
Publisher: YUZU
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Chapter 8, Problem 7LTB
Summary Introduction

Introduction:

DNA is a chain of polynucleotide. A nucleotide is made up of a deoxyribose sugar, anitrogenous base on the first carbon of sugar and a phosphate group on the fifth carbon ofthe sugar. The nucleotides are of four types based on the type of the base. Thenitrogenous bases are either purines (adenine and guanine) or pyrimidines (cytosine andthymine). Based on the arrangement of bases, the DNA sequence is determined. The DNA arrangement of every individual is unique to them, which can be used for the DNA profiling.

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Please answer these two questions regarding PCR:   a) Why do you need to perform PCR on DNA obtained from a crime scene? b) Why so forensic labs analyze non-coding DNA rather than genes?
Match the following terms with their definitions and label each component of the PCR mixture in the diagram (use the letters A-D):I. DNA polymeraseII. PrimersIII. NucleotidesIV. Genomic DNA template A. DNA that contains the target sequence that will be replicated using PCR.B. An enzyme that copies the DNA sequence.C. A mixture of 4 nucleotides (A,G,C, and T) that will be polymerized into the replicated DNA sequence.D. A short DNA sequence that allows the enzyme to bind and initiate polymerization.
For each situation, write the letter of the technique that would be most helpful; A. DNA editing A doctor wants to know if a patient has an inherited using CRISPR B. DNA replication using PCR C. DNA analysis through genetic testing D. DNA insertion 16. disorder. I A scientist needs many copies of a gene to conduct an 17. experiment. A genetic engineer wants to replace a defective copy of a gene with a functional copy in a chromosome. 18. into bacteria as a plasmid A medical researcher needs many copies of a protein 19. (insulin) to be produced to use in a medical treatment. A researcher crossed two purebred shrubs of the same species. One produces a fruit with a thin skin, and one produces a fruit with a thick skin. All of the plants resulting from the cross produce fruits with thick skins. Enter one letter in each blanks (19 & 20) to correctly complete the sentences. ninate Education TM, Inc.
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