MACROSCALE+MICRO.ORG...-OWLV2 ACCESS
7th Edition
ISBN: 9781305884175
Author: Williamson
Publisher: CENGAGE L
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Chapter 8, Problem 7Q
What will be the result of applying too much compound to a TLC plate?
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What is a guard column and why is it used in HPLC but not in GC systems?
The solvent level in the development chamber should be above the level of the spotted compounds on the TLC plate.
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As solvent polarity increases, the Retention Factor (Rf) of compounds on the TLC plate increases.
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Chapter 8 Solutions
MACROSCALE+MICRO.ORG...-OWLV2 ACCESS
Ch. 8 - Prob. 1QCh. 8 - Prob. 2QCh. 8 - What problem will ensue if the level of the...Ch. 8 - Prob. 4QCh. 8 - Prob. 5QCh. 8 - Arrange the following in order of increasing Rf...Ch. 8 - What will be the result of applying too much...Ch. 8 - Prob. 8QCh. 8 - Prob. 9QCh. 8 - A TLC plate showed two spots with Rf values of...
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- What are some ways to visualize a TLC plate? Why may it be necessary to have more than one method to visualize a TLC plate?arrow_forwardWhat is the retention factor (Rf) of the most polar and the least polar spot present in the standard ink?arrow_forwardWhich of the following methods cannot be used to visualize spots on a TLC plate? Group of answer choices UV light Iodine vapor chamber stain/spray flashlightarrow_forward
- What happens if the spots are made too small when preparing a TLC plate for development?arrow_forwardWhich statement is true? Statement 1: Since tiny amounts of material are exposed on an open surface, the use of TLC is limited to relatively nonvolatile substances.Statement 2: To determine the identity of a specific compound in a mixture, the TLC behavior of the test sample and that of authentic standard compounds are compared on the same plate.arrow_forwardHow you can improve resolution in HPLC analysisarrow_forward
- Can an HPLC run be stopped during operation without impairing the separationarrow_forwardWhy is it important to handle the TLC plate in such a way that your fingers do not come in contact with the coated surface of the plate?arrow_forwardHow is TLC used to determine the purity of a sample?arrow_forward
- What happens if the spots are made too large when preparing a TLC plate for development?arrow_forwardIt is acceptable to draw the TLC spotting line with a pen as long as it is marked very lightly. True Falsearrow_forwardYou are treating the TLC plate with 5% of sulfuric acid in ethanol. Briefly describe how this works.arrow_forward
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