Macroscale and Microscale Organic Experiments
7th Edition
ISBN: 9781337329552
Author: Kenneth L. Williamson; Katherine M. Masters
Publisher: Cengage Learning US
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Textbook Question
Chapter 8, Problem 7Q
What will be the result of applying too much compound to a TLC plate?
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What is a guard column and why is it used in HPLC but not in GC systems?
The solvent level in the development chamber should be above the level of the spotted compounds on the TLC plate.
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What are some ways to visualize a TLC plate? Why may it be necessary to have more than one method to visualize a TLC plate?
Chapter 8 Solutions
Macroscale and Microscale Organic Experiments
Ch. 8 - Prob. 1QCh. 8 - Prob. 2QCh. 8 - What problem will ensue if the level of the...Ch. 8 - Prob. 4QCh. 8 - Prob. 5QCh. 8 - Arrange the following in order of increasing Rf...Ch. 8 - What will be the result of applying too much...Ch. 8 - Prob. 8QCh. 8 - Prob. 9QCh. 8 - A TLC plate showed two spots with Rf values of...
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- As solvent polarity increases, the Retention Factor (Rf) of compounds on the TLC plate increases. Group of answer choices True Falsearrow_forwardWhat is the retention factor (Rf) of the most polar and the least polar spot present in the standard ink?arrow_forwardWhich statement is true? Statement 1: Since tiny amounts of material are exposed on an open surface, the use of TLC is limited to relatively nonvolatile substances.Statement 2: To determine the identity of a specific compound in a mixture, the TLC behavior of the test sample and that of authentic standard compounds are compared on the same plate.arrow_forward
- What happens if the spots are made too small when preparing a TLC plate for development?arrow_forwardHow you can improve resolution in HPLC analysisarrow_forwardWhich of the following methods cannot be used to visualize spots on a TLC plate? Group of answer choices UV light Iodine vapor chamber stain/spray flashlightarrow_forward
- How is TLC used to determine the purity of a sample?arrow_forwardWhy is it important to handle the TLC plate in such a way that your fingers do not come in contact with the coated surface of the plate?arrow_forwardWhat happens if the spots are made too large when preparing a TLC plate for development?arrow_forward
- Can an HPLC run be stopped during operation without impairing the separationarrow_forwardIt is acceptable to draw the TLC spotting line with a pen as long as it is marked very lightly. True Falsearrow_forward1. TLC a) What are the results of loading too much compound to a TLC plate?b) What would happen if you added too little compound to a TLC Plate?arrow_forward
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