BROCK BIOLOGY OF MICRO(LL)W/MOD.MASTER.
15th Edition
ISBN: 9780134773384
Author: MADIGAN
Publisher: PEARSON
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Textbook Question
Chapter 8.4, Problem 1CR
Describe the events that occur on an agar plate containing a bacterial lawn when a single bacteriophage particle causes the formation of a bacteriophage plaque.
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Define the following terms:
Bacteriophage
λ DNA
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Outline the events that lead to formation of a plaque in a lawn of bacterial cells
Describe the infective process of a bacteriophage as it attacks a cell.
Chapter 8 Solutions
BROCK BIOLOGY OF MICRO(LL)W/MOD.MASTER.
Ch. 8.1 - How does a virus differ from a cell?Ch. 8.1 - Why does a virus need a host cell?Ch. 8.1 - Compared with cells, what is unusual about viral...Ch. 8.1 - Once inside a host prokaryotic cell, what are the...Ch. 8.2 - Distinguish between a capsid and a capsomere. What...Ch. 8.2 - What is the difference between a naked virus and...Ch. 8.2 - What kinds of enzymes can be found within the...Ch. 8.2 - Where does the envelope surrounding animal viruses...Ch. 8.3 - What is packaged into capsids during maturation?Ch. 8.3 - Explain the term burst size.
Ch. 8.3 - Prob. 3MQCh. 8.3 - Why does a one-step growth curve differ in shape...Ch. 8.4 - What is meant by a viral titer?Ch. 8.4 - What is a plaque-forming unit?Ch. 8.4 - What is meant by the term plating efficiency?Ch. 8.4 - Describe the events that occur on an agar plate...Ch. 8.5 - How does attachment contribute to virushost...Ch. 8.5 - Prob. 2MQCh. 8.5 - Prob. 3MQCh. 8.5 - What is required for a bacteriophage T4 virion to...Ch. 8.6 - Prob. 1MQCh. 8.6 - Give one example each of T4 early, middle, and...Ch. 8.6 - What is required to package the T4 genome into its...Ch. 8.6 - Bacteriophage T4 lacks its own RNA polymerase. How...Ch. 8.7 - What is a lysogen and what is a prophage?Ch. 8.7 - How does DNA replication in lambda differ from...Ch. 8.7 - What commits lambda to the lytic versus the...Ch. 8.7 - What enzyme is required to form a prophage, and...Ch. 8.8 - Prob. 1MQCh. 8.8 - What is the difference between a persistent and a...Ch. 8.8 - Prob. 3MQCh. 8.8 - Why can it be said that the retrovirus genome is...Ch. 8 - What causes the viral plaques that appear on a...Ch. 8 - The promoters on genes encoding early proteins in...Ch. 8 - Under some conditions, it is possible to obtain...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- What other factors may control the outcome of an infectiousdisease?arrow_forwardIn a petri dish with solidified agar with escherichia coli, enterobacter aerogenes and staphylococcus aureus, you streak a loopfull of lytic T4-phage in a single line onto the center of the the dish, how do you know if bacteriophage infected the bacteria. why didn't the bacteriophage infect all 3 bacteria?arrow_forwardDescribe one scenario in which antibiotic resistant bacteria in a chicken could enter your body.arrow_forward
- Define the following terms:a. transpositionb. transposable elementc. bacterial transformationd. transductione. conjugationarrow_forwardWhich of the following terms describes bacteriophage DNA that has become integrated into the host cell chromosome? A) plasmid B) impolite phage C) transposon D) prophagearrow_forwardDescribe the appearance of the area surrounding the bacterial growth of b.cereus on left and b.megaterium on right.arrow_forward
- Why are encapsolated bacteria generally more pathogenic than un-capsolated strains. a. Because capsule allows the organism to hide in the body of the host. b. because the capsule causes the phagocyte to mutate c. because the capsule protects bacteria from host immune cells d. because the capsule kills the phagocytes e. because the capsule stimulates a potent immune response in the host.arrow_forwardWhich of the following experiments was used by Twort and d'Herelle to demonstrate the existence of bacteriophages? a. Examination with an electron microscope b. Examination with the light microscope c. Mixing phages with bacteria in liquid media d. Mixing phages with bacteria on solid agararrow_forwardWhich of the following is TRUE when one assay bacteriophage titers? You should: a) first mix the phages with a live bacterial culture and then pour the mixture on the agar plate b) directly add the phage dilution onto the surface of an agar plate c) add tryptic soy broth to the phage dilution and incubate overnight d) incubate a phage solution with live bacterial cells for several minutes. You must add soft agar to the mixture before pouring the content on the agar platearrow_forward
- Why must the top agar be supplemented with maltose? A) Maltose allows for binding of the phage to the E. Coli and aids in the infection process B) Maltose helps stabilize the phage within the mediaarrow_forwardWhy is it important to know if the bacteria is encapsulated or not?arrow_forwardIn which bacteriophage life cycle does the phage DNA become incorporated into the bacterial chromosome? a. Lytic b. Lysogenic c. Both lytic and lysogenic d. Neither lytic nor lysogenicarrow_forward
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