BROCK BIOLOGY OF MICRO(LL)W/MOD.MASTER.
15th Edition
ISBN: 9780134773384
Author: MADIGAN
Publisher: PEARSON
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Chapter 8.4, Problem 3MQ
- What is meant by the term plating efficiency?
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Chapter 8 Solutions
BROCK BIOLOGY OF MICRO(LL)W/MOD.MASTER.
Ch. 8.1 - How does a virus differ from a cell?Ch. 8.1 - Why does a virus need a host cell?Ch. 8.1 - Compared with cells, what is unusual about viral...Ch. 8.1 - Once inside a host prokaryotic cell, what are the...Ch. 8.2 - Distinguish between a capsid and a capsomere. What...Ch. 8.2 - What is the difference between a naked virus and...Ch. 8.2 - What kinds of enzymes can be found within the...Ch. 8.2 - Where does the envelope surrounding animal viruses...Ch. 8.3 - What is packaged into capsids during maturation?Ch. 8.3 - Explain the term burst size.
Ch. 8.3 - Prob. 3MQCh. 8.3 - Why does a one-step growth curve differ in shape...Ch. 8.4 - What is meant by a viral titer?Ch. 8.4 - What is a plaque-forming unit?Ch. 8.4 - What is meant by the term plating efficiency?Ch. 8.4 - Describe the events that occur on an agar plate...Ch. 8.5 - How does attachment contribute to virushost...Ch. 8.5 - Prob. 2MQCh. 8.5 - Prob. 3MQCh. 8.5 - What is required for a bacteriophage T4 virion to...Ch. 8.6 - Prob. 1MQCh. 8.6 - Give one example each of T4 early, middle, and...Ch. 8.6 - What is required to package the T4 genome into its...Ch. 8.6 - Bacteriophage T4 lacks its own RNA polymerase. How...Ch. 8.7 - What is a lysogen and what is a prophage?Ch. 8.7 - How does DNA replication in lambda differ from...Ch. 8.7 - What commits lambda to the lytic versus the...Ch. 8.7 - What enzyme is required to form a prophage, and...Ch. 8.8 - Prob. 1MQCh. 8.8 - What is the difference between a persistent and a...Ch. 8.8 - Prob. 3MQCh. 8.8 - Why can it be said that the retrovirus genome is...Ch. 8 - What causes the viral plaques that appear on a...Ch. 8 - The promoters on genes encoding early proteins in...Ch. 8 - Under some conditions, it is possible to obtain...
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- How would you make two-fold serial dilutions such that the last tube is a 1:32 dilution of the original, concentrated material? Assume that you need to have at least 500 µl of each dilution, and you should be able to perform the dilutions in microfuge tubes with a maximum capacity of 1.5 ml.arrow_forwardWhat are some methods to prevent contamination during streak plating a medium?arrow_forwardOf the two principal polymers used in column chromatography and electrophoresis, which one would be most immune to contamination by bacteria and other organisms?arrow_forward
- What is one advantage of a pour plate over a streak plate?arrow_forwardWhat are the underlying physical principles of paper chromatography? What is spectrophotometry, the essence of it?arrow_forwardWhat two procedures should you routinely carry out to verify the purity of your working stock?arrow_forward
- Why is immobilization a good strategy for dealing with uraniumpollution?arrow_forwardwhat is the effect and rationale when three milliliters instead of 1 mL was placed in the Petri dish in pour-plate method?arrow_forwardWhy is the annealing temperature different between samples? How is this computed?arrow_forward
- What is the purpose of doing the silver nitrate test? Write the chemical equation involved herearrow_forwardAn unknown sample was titrated with CH3COOH. After making the titration curve, no steep change was observed. This means that the unknown sample must be a _____.arrow_forwardwhat is the is the dilution factor when you add 2 mL ssample of 8 mL diluent?arrow_forward
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