Chapter 9, Problem 33PDQ

Assume that you are interested in separating short (25–40 nucleotides) DNA molecules from a pool of longer molecules in the 900–1000 nucleotide range. You have two recipes for making your polyacrylamide gels; one recipe uses 12 percent acrylamide and would be considered a “hard gel,” while the other uses 4 percent acrylamide and would be considered a loose gel. Which recipe would you consider using and why?