Agar

Pseudomonas aeruginosa in the Blood Agar is expected for the color of the media to stay the same. The colony should be grayish green, and it should produce a beta-hemolysis reaction, which completely destroys all the red blood cells and clears the Blood Agar. In the Brilliant Green Agar, the color of the media should stay the same. The colony should be pink, and it should be gram negative. In the Eosin Methylene Blue Agar, the color of the media should stay the same. There should be no growth in

The medium LB Agar was used. Fisher Scientific ® Lysogeny Broth or “LB Agar” was created by suspending forty grams per one liter of purified water. Next, the LB Agar was mixed and heated using a magnetic mouse and a Corning ® Stirrer/Hotplate until the solution was transparent and beginning to bubble towards the top of the Erlenmeyer flask. Next, the flask with the mixed LB Agar was removed from the Corning ® Stirrer/Hotplate. The LB Agar filled up large test tubes in a large

Genus theyre working with. This indicates which tests to perform next. The MacConkey agar is a selective medium that only allows the growth of gram positive bacteria confirming the results received from the gram staining procedure. The NaCl growth test indicates whether or not the organism is able to

Course: BIO-205 BD2 Microbiology Instructor: Dirk VandePol Date: 6/21/2013 Streak Plate Isolation for Obtaining Pure Culture 1. When an agar plate is inoculated, why is the loop sterilized after the initial inoculation in put on? Ans: We use agar plate to inoculate microbes by zipping the loop on the agar several times. We streak on the agar plate four time, propose is to isolate the unknown bacteria. Therefore, the first time to streak on the plate, there are million of bacteria on the

Eosin Methylene-Blue agar used for the growth of Escherichia coli. These bacteria are commonly found in places of fecal contamination (CDC). A control group of Escherichia coli was used as well as swabs from the student’s phone and hand. Another type of media used in this experiment is Differential/Selective media. This media makes detection easier after the incubation period has occurred. An example of differential/selective media used in this experiment was Mannitol Salt agar used to isolated the

organisms can easily be seen using differing types of agar, which creates an ideal environment for the organisms to form colonies, which are groups of hundreds of organisms that can be seen with the naked eye. In order to see individual microorganisms, it is necessary to use the magnification of a high-powered microscope. These techniques of microbiology are used in the following five experiments. The first experiment used Trypticase Soy Nutrient Agar (TSA), which can grow a wide variety of organisms

this lab. Find each section and complete the “Preparing for Class” sections. Preparing for class - Day 1 Read in your lab manual about the following agar mediums: Blood Agar (pg 168), EMB Agar (pg 170), Mannitol Salt Agar (MSA)(pg 172) ), MacConkey Agar (pg 174), and PEA Agar (pg 176) to answer the following: 1. What does the blood agar select for?

Professor handed out one unknown organism to each of the students. Students had to record their own unknown ID. Used the aseptic techniques to perform the gram stain and inoculate the nutrient media(nutrient broth, nutrient agar slant, and nutrient agar plate). Only for the nutrient agar plate media used four quadrant streak method. Gram stain was distinguishing the gram-positive or gram-negative cells. Preparing of the heat-fixed slide was taking some times while air dries it. Therefore, inoculated the

How does the addition of pGLO plasmid to a solution containing E. coli bacteria affect the growth and characteristics of the bacteria? Genetic transformation is the incorporation of foreign DNA into an organism to potentially change the organism’s trait. Plasmids are small circular DNA that replicate separately from the bacterial chromosome. In nature, these plasmids can be transferred between bacteria allowing for the sharing of beneficial genes. Due to this characteristic, plasmids allow for genetic

Abstract The identification of bacteria is a crucial procedure in the field of biology. Identification can be done through a series of tests run on different forms of media that detect the physical and biochemical properties of the cells. In this experiment, a sample containing two unknown species of bacteria was run through fourteen tests to determine morphology, fermentation properties, reducing and oxidative properties, and other various characteristics of each isolated unknown. It was concluded