Agar

information is accurately processed and presented, experimental procedures are planned, designed and evaluated properly, producing valid results, recording results, and valid conclusion is drawn. 3. To develop the aseptic techniques for preparing agar plates and bacterial culture. Problem statement Do antibiotics have the same effect on killing different types of bacteria? Abstract: The main objective of this experiment is to investigate the effect of different types of antibiotics on bacteria

petri dishes were prepared to cross the isolates, producing perithecia. First, two 0.5 cm squares of fungal hyphae containing agar were cut from a cultured petri dish. Wild Type and Tan isolates were used for the control; X-ray samples corresponding to the correct treatment were used for the experimental plates. The agar squares were then placed with the hyphae side facing the agar on the quadrant labeled for the corresponding isolate. These steps were then repeated for the remaining strains/ culture

Physarum Polycephalum is a slime mold that has a yellow coloring to it and it resides in areas of shade, cool temperatures and moisture. Not only does this slime mold have the name Physarum Polycephalum, but it is also referred to as Polycephalum as suggested by (Jabr, 2012). P. Polycephalum is a multicellular microbe that feeds fungal spores and other microbes because the organisms are heterotrophic (Sauer, 1986). P. Polycephalum is very interesting because it has traits of both fungi and animals

as the surface area to volume ratio decreases throughout evidence supported. There is a pattern in table 5 shows that, the surface area to volume ratio decreases when the agar jelly increases in size from 1cm to 3cm cube by a ratio of 6 to a ratio of 2, showing that average rate of diffusion which decreases as the size of the agar increases has a linkage to cells as cells increase in sizes. ‘If a cell grows beyond a certain size, materials will not be able to pass through the membrane fast enough to

4-3 Mannitol Salt Agar is used to differentiate Staphylococcus areus from other microorganisms. The agar contains mannitol, salt, and a red pH indicator. Most organisms cannot grow in such a salty environment, but organisms of the genus Staphylococcus are uninhibited, making the agar selective for Staphylococcus. Therefore, if an organism is able to grow well on the agar, it is likely that it is Staphylococcus. The agar is differential because of the mannitol and the indicator. Only Staphylococcus

diarrhoea, myalgia, hypotension and signs of septic shock. In order to identify possible bacteria responsible for these symptoms, a vaginal swab was sampled, incubated on agar overnight and stored at 4C, after which they were examined throughout a series of microbiological tests. The initial culturing of the sample on nutrient agar indicated two organisms were present, and hence these were analysed individually. A Gram stain was applied to each bacterium and they were viewed individually under microscope

manufactured specifically to fight the harmful nature of the bacteria. However, after concluding the investigation, the data and evidence collected potentially disproves the initial hypothesis. The investigation was similar to the Kirby-Bauer test, or the Agar diffusion test, in which the group used three natural antibacterial agents and two synthetic agents. Natural agents were lemon, garlic and tea tree oil, while synthetic agents were bleach and hand sanitizer.

order to isolate fecal coliforms, Eosin Methylene Blue agar, which contains peptone, lactose, sucrose, and the dyes eosin Y and methylene blue, is used. These sugars provided encourage growth of fecal coliforms while the dyes inhibit growth of Gram-positive organisms. As a result, EMB agar allows the inoculation of Escherichia coli, Enterobacter aerogenes, Salmonella typhimurium, and Enterococcus faecalis. To identify E. coli strains on EMB agar, the cultivated bacteria must appear green, black, or

can flourish when salt or sugar concentrations goes up or down, however too much in either direction can be detrimental to the microbe. Materials: • Nutrient agar • Nutrient agar with 0.5% sucrose • Nutrient agar with 30% sucrose • Nutrient agar with 60% sucrose • Nutrient agar with 0.5% NaCl • Nutrient agar with 15% NaCl • Nutrient agar with 30 % NaCl • 5 Petri dishes per group (40 total) • Sterile applicator sticks • Hot plate • 1000 mL beaker • Wax pencil • Bunsen burner • Hose • Flint Striker

relationship between volume/Cm3, surface area, and diffusion using agar cubes measured in time/s; and to demonstrate, using diffusion, why the size of cells is limited, keeping the room temperature and pressure constant. Hypothesis: I expect to find that when the surface area to volume of a cell reaches a certain level, diffusion alone won't be able to supply