Agar

100 ml of agar media. MATERIALS REQUIRED: Beaker, distilled water, stirrer, measuring cylinder, weighing machine. COMPONENTS OF AGAR MEDIA: ➢ Peptone 0.5gm ➢ Beef extract 0.3gm ➢ NaCl 0.5gm ➢ Agar powder 2gm THEORY: Nutrient agar media is used to facilitate the growth of the wide range of non-fastidious bacteria. 0.5% Peptone - provide organic nitrogen 0.3% beef extract/yeast extract - the water-soluble content of these contribute vitamins, carbohydrates, nitrogen, and salts 1.5% agar - this gives

For this experiment we utilized a variety of materials. We first utilized a sharpie pen to write down the locations of our experiments and our names among other information on seven different Agar plates. We then used seven different containers of sterilized water to make sure there was no contamination between different samples. We also utilized seven different disposable swabs for this exact same reason. We also used seven packages of Para-film in order to seal the plates. Lastly we made use of

Drosophila melanogaster stocks used for the behavior assays (2-choice assay and tracking assay) and molecular analysis (qRT-PCR and immunohistochemistry), include the wild type Canton-S (CS) line, the UAS-GABA (B)-RX-RNAi (Root et al., 2008) (where X represents receptor subtype 1, 2, or 3), and Or X-Gal4 lines (Or 47a Gal4 and Or 42b Gal4), GH-146 Gal4, Orco-Gal4, 10x; UAS-CD8; GFP were purchased from the Bloomington stock center (http://flystocks.bio.indiana.edu). Virgin female flies from UAS-GABA

The claim to this Agar Pieces lab is that the cells that have a larger surface area to volume ratio are more efficient at diffusing essential nutrients. Another solution, is the rate of diffusion is related to cell size. Nutrients diffuse at a faster rate through small cells than they do through large cells. In this lab of Agar gel, the purpose of this experiment is to find the surface area and volume of the cell that may affect the ability of the molecule to be able to diffuse the cellular

MATERIAL and METHODS: Aseptic Technique and Culturing Microbes(1) experiment was followed as stated in the lab manual from Clinical Microbiology Class C-453. Aseptic technique was initiated at the beginning of each experiment. Starting by cleansing the work surface with disinfected wipes to prevent cross contamination each time. Utilizing the gloves and personal protective equipment assisted in maintaining a pure culture during the series of experiments. The first step, was to grow the yeast

Discussion: This lab shows ‘diffusion’ of agar cube. The agar contains high concentration of solute (phenolphthalein) and the sodium hydroxide has lower concentration of solute (phenolphthalein). So as the phenolphthalein in the agar cube diffuses out to the sodium hydroxide, which turns the color of liquid, the sodium hydroxide diffuses into the agar cube that turns the color of agar cube as well. Based on the result, the optimum size for a cell is small size as they could be. The reason why is

in petri dishes filled with agar which were prepared one day before in advance with the expectation that after one week, distinct bacterial colonies will grow larger in numbers so that the human can see them. These single-celled prokaryotes were grown on agar as it is easy to culture and grow bacterial colonies in nutrient agar, a gelatin-like substance with a semi solid surface on which bacteria can grow and reproduce quickly while consuming added nutrients in the agar mixture. The petri dishes were

this study are of analytical grade, Table 3.1 shows the chemicals used in the study and their manufacturing companies. Table 3.1 Chemicals used in the present study. Chemicals Manufacturing companies Sodium acetate Sigma, USA Yeast extract Lab M, UK Agar B & V srl-Parma, Italy Acetic acid ADWIC, Egypt Glucose Sigma, USA GABA Loba, India MSG Sigma, USA DEAE-Cellulose Sigma, USA Catalase enzyme Biodiagnostic, Egypt APTT kit & PT kit Biomed diagnostics, Germany n-butanol Sigma, USA Glycerol Acros, USA

Introduction The primary goal of this lab was to notice the bacteria growth in each tube/plates and to be able to properly inoculated the media to get a good result. Materials • 4 Nutrient Broths • 4 Agar Slants • 4 Agar Deeps • 4 Petri Dish • 1 loop and needle • Bacillus subtilis, Escherichia coli, Staphylococcus aureus Samples Procedures 1. Label all of the tubes and petri dishes with the name of the bacteria and my lab partner and I initial, but leave one of each media as a control and label

27). Microbiological Media: a) Nutrient Agar (NA) Composition of Nutrient Agar • 0.5% peptone • 0.3% beef extract/ Yeast extract • 1.5% agar • 0.5% NaCl • Distilled water pH is adjusted to neutral (7.4) at 25°C Preparation of Nutrient Agar • Suspend 28g of nutrient agar powder in 1L of distilled water. • Heat this mixture while stirring to fully dissolved all components • Autoclave the dissolved mixture 121°C for 15 minutes • Pour nutrient agar into each plate and leave plates on the sterile