objective of this experiment was to determine the effects of varying pH values on the enzymatic activity of glucose oxidase. This enzyme specifically oxides D-glucose while simultaneously reducing oxygen into hydrogen peroxide, whose catalysis by the enzyme horseradish peroxidase coupled with this reaction is broken down into its constituent parts of water and oxygen. The reaction rate of glucose oxidase was tested at four distinct pH levels of 3, 4, 5, and 6. The absorbency of each solution was determined
Introduction: PH sensors are used in many scientific laboratories to measure the hydrogen-ion concentration of a liquid substance and to determine the basicity and acidity of a liquid. PH meters measure the electrical potential difference between a reference electrode probe and a pH electrode probe. Since these probes are very sensitive, they are required to be kept cleansed from contaminants. If exposed to contamination, one risks faulty or unreliable data, since their accuracy is dependent on a
Lab Report Background Research pH is a measure of the potential hydrogen ion concentration of a solution. Solutions that have low pH’s or a ph level below 7 are considered acidic. Solutions that have a high pH level or above 7 are considered basic. The pH scale measures how acidic or basic a solution may be. Some examples of solutions that have high acidity and a low pH level are Battery acid, lemon juice, and vinegar. Some solutions that are basic and a high pH level are soapy water, baking soda
The Effects of pH, Temperature, and the inhibitor Hydroxylamine on Brassica rapa Peroxidase Enzyme Sriya Bhamidipati Abstract. Peroxidase is an enzyme whose main function is to break down hydrogen peroxide. It is rich in content in the organism Brassica rapa, or the turnip. The purpose of this study was to investigate and observe the effects of pH, temperature conditions and the inhibitor Hydroxylamine on Peroxidase ‘s ability to break down Hydrogen Peroxide. By observing the absorbance change
different pH levels affect the population growth of yeast, which was based on the mm of carbon dioxide produced. Through this, our independent variable was the pH level of solution added to the test tube of yeast and sugar. Our dependent variable was the mm of CO2 produced, indicating population growth. For this lab, the proper control was a test tube with the yeast and sugar but no pH solution added. This was useful to compare our data to because it wasn’t being changed by the pH. Therefore
The water sample with the lowest pH was the Dhaka water sample, which had a pH of 2.52, making it the most acidic water. This was followed by Port River water, which had a pH of 4.6, Waterfall Gully water sample at 4.75, and Johnston 's Creek at 5.29 (refer to Table 1 and Figure 3). The Dhaka sample 's pH is between the acidity of vinegar and orange juice (ScienceBuddies, 2015). Figure 3: Average pH/acidity of the water samples. Not all samples were titrated, as both Waterfall Gully and Johnston
Introduction/Hypothesis: This experiment was conducted to observe the effects of certain factors on enzymatic activity in yeast cells, namely environments with varying pHs (Thomasch). Enzymes play a large role in many different biochemical processes that occur throughout many different organisms. As a distinct subcategory of proteins, they are composed in the same manner as any other protein, with amino/carboxyl groups, and variable side chains that ultimately determine their structure,
High pCO2, low pH groundwater discharge drives natural acidification of reefs Christina M. Richardson, Henrietta Dulai, Brian Popp, and Kathleen Ruttenberg Abstract Diel changes in the marine carbonate chemistry of reefs are thought to be primarily biologically-driven. The presence of groundwater discharge in nearshore reefs may complicate our understanding of carbon cycling in these systems, however. Here we show that coastal groundwater inputs shift and heighten biologically-induced changes in
How does soil affect the pH of water? Abstract: During this lab, the pH of water in soil from a man made garden, a deciduous forest, and a river bank were tested after leaving it in containers for one, two, and three hours, coming out to a total of three trials with three different soils all together. After testing the pH of the water when being added to the soil for the desired amount of time and comparing it to the original water with no soil added, is then when each pH difference was observed and
This experiment is being done to show the effect oh pH levels on daphnia by changing pH levels and measuring the hear rate. Introduction Daphnia magna are commonly used to test water toxicity, they are a vital part of fresh water ecosystems. They serve as a crucial role in food webs as they are a food source for smaller fish and invertebrates, and they also are a consumer of algae and bacteria (Elbert, 2005). They range from 2-5mm long these small crustaceans are also referred to as water fleas