Transfection

their toxicity. In the present study, the toxicity of three different polymers that represent promising drug carriers will be evaluated using 2D, 3D cell cultures and zebrafish embryos. The polymers to be studie include the well-known cationic transfection agents Poly(ethylenimine) (PEI) and poly(ethylene glycol) (PEG) for their established cytotoxicity as well as the uncharged drug carrier material pHPMA (i.e. poly(N-2-hydroxypropyl) methacrylamide), Poly(ethylene glycol)-b-poly(N-(2-hydroxypropyl)

In the spring of 1938, one of the deadliest pandemics first emerged along the shores of Italy. Through the examination of historical documents regarding this pandemic, it was discovered that the Black Death, or the plague, was initially spread among several Italian merchant ships on the return to the city of Messina from different regions of Asia, and such a sickness was a mystery to the sailors, as well as to the citizens, whom have succumbed to it (“The Black Death”). After a period of three years

1. The admissions committee is interested in gaining more insight into you as a person. Please describe a significant personal challenge you have faced, one which you feel has helped to shape you as a person. Examples may include a moral or ethical dilemma, a situation of personal adversity, or a hurdle in your life that you worked hard to overcome. Please include how you got through the experience and what you may have learned about yourself as a result. (3500 char) “I don’t know” I can still distinctly

Vascular endothelial factor (VEGF) is a potent angiogenic factor which plays an important role in regulating normal physiological and pathological angiogenesis. Correctly timed expression of VEGF at appropriate levels is crucial for normal development of vasculature and homeostasis, but also vital for solid tumour growth. VEGF is highly expressed in solid tumours and is required for the development and maintenance of blood vessels within the tumour, which is a prerequisite for successful tumour

Review: Biodegradable Nanoparticles for Drug Delivery Introduction With the many innovations in the disciplines of nanotechnology and drug delivery their merger is a very advantages strategy transport of small molecular weight drugs and macromolecules for localized or targeted delivery to the tissue of interest [1]. Nanotechnology will provided an outlet for conveying therapeutic agents in biocompatible nanocomposites such as nanoparticles, nanocapsules, micellar systems, and conjugates. With

Herbert Boyer and Stanley Cohen first established the Genetic Modification of Organisms in 1973. The successful accomplishment arrived after thousands of years of selective breeding in which farmers used unreliable methods that took many years to achieve the desired characteristic within an organism. However genetic modification allows specific individual genes to be transferred from one organism to another instantly and occurring in the same generation of the organism. The insertion, deletion and

Cell cycle progression is orchestrated by a complex convergence of many signaling pathways. The decision to activate particular pathways while inhibiting others is mediated by a select group of gatekeeping proteins. At the molecular level, one particular interesting area of research is identifying particular proteins that function as crossroads of t Diverse pathological conditions, such as cancer and atherosclerosis, share a common molecular basis: deregulation of cell cycle progression . In

Bioethics is studied to find ethical reasoning in life science and the experiments that are done in this field to further understand concepts in this subject. Many controversial topics surround bioethics, because even though some forms of science technology and research can help to understand biological concepts better, they can have negative drawbacks on the subject experimented on and the group involved around it. This creates a difficult triangle of choice between what will be best for science

III. Experimental Procedures 1. Determination of direct binding between FMRP and Cyclin E We hypothesize that FMRP interacts with Cyclin E mRNA. To investigate this, we propose to perform bimolecular fluorescence complementation (BiFC) in Xenopus oocytes. Xenopus oocytes have been previously used to demonstrate that FMRP binds to mRNA in the nucleus24. Xenopus oocytes are easily extracted from adult females and are tolerant of being injected with numerous RNA and DNA constructs. Furthermore, the

Neonatal cerebral hypoxia-ischemia (HI) remains a major cause of death and neurological disability. Neural stem cells (NSCs) shows great promise in restoring HI-associated neurological tissue damage and hypothermia is reported to be beneficial for HI recovery in animal studies. We aimed to investigate whether mild hypothermia after neonatal cerebral hypoxia-ischemia (HI) could enhance the therapeutic effect of NSCs transplantation with a neonatal HI mouse model. Postnatal day 7 mice underwent right