Questionnaire

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School

Brooklyn College, CUNY *

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Course

2110

Subject

Biology

Date

Feb 20, 2024

Type

pdf

Pages

2

Uploaded by BrigadierHeat16835

Questionnaire Answer the questionnaire in your own words. Quoting the article will get you an automatic zero for the given question. 1. What is the title of the article? (5 pts) Proteomics Identification of Differentially Expressed Leaf Proteins in Response to Setosphaeria turcica Infection in Resistant Maize 2. Who are the authors of the study? (5 pts) ZHANG Xiao-li, SI Bing-wen, FAN Cheng-ming, LI Hong-jie and WANG Xiao-ming 3. What are the authors trying to figure out with the study? (10 pts) When resistant maize is infected with Setosphaeria turcica, the authors of the article are attempting to identify the differentially expressed leaf proteins. They are attempting to comprehend the molecular mechanism underlying maize's resistance to Setosphaeria turcica. 4. Briefly outline the methodology used in the study. Which of the techniques we see in class do the authors use in the study? (10 pts) The methodology used in the study includes the following steps: Sample collection and preparation Protein extraction and quantification Two-dimensional electrophoresis (2-DE) Image analysis and protein identification using mass spectrometry Bioinformatics analysis The authors use two-dimensional electrophoresis (2-DE) in the study, which is a technique we have discussed in class.
5. Briefly discuss what the major findings are. (10 pts.) The major findings of the study are: 29 differentially expressed proteins were identified in response to Setosphaeria turcica infection in resistant maize. These proteins are involved in various biological processes, including defense response, metabolism, and protein folding. The authors propose a model for the molecular mechanism underlying the resistance of maize to Setosphaeria turcica. 6. Why is this research important? (10 pts) This research is important because it provides insights into the molecular mechanism underlying the resistance of maize to Setosphaeria turcica. This information can be used to develop new strategies for controlling Setosphaeria turcica infection in maize, which is a major disease affecting maize production worldwide.
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