F23 Exp 10 Lab Report(1)

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University of Texas, Dallas *

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3380

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Biology

Date

Feb 20, 2024

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docx

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6

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BIOL 3380 Experiment 10 - Lab Report Enzyme Kinetics of LDH Name: Circle your lab section: T-AM W-AM R-AM F-AM T–PM W-PM R-PM F-PM Instructor: Jing Pan Graduate TA: Date: 11/25/2023 Partner:
Experiment 10 – Lab Report Enzyme Kinetics of LDH Edit this word document by inserting your answers after each question/space. Please denote your answers by using bold , italic s, or colored font. Do NOT renumber/reorganize the questions. “Showing your work/calculations” can be achieved by typing it out, pasting in a clear image of your handwritten work, or using handwritten annotation on the document (if you have the technology to do so). Insert graphs (handwritten on graph paper or electronically constructed with horizontal/vertical gridlines) as a clear image with appropriate titles, labeled/scaled axes, and standard deviation bars when appropriate. All figures/graphs/tables must include appropriate titles, legends, and labeled/ scaled axes. 1. (3pts) Present a table showing initial velocity data ( A 340nm /min, NADH[nM]/min, and ∆NADH[nmol]/min) as a function of LDH concentration. 2. (3pts) Present a graph of NADH[nmol]/min as a function of LDH concentration.
3. (3pts) Present a table showing initial velocity data ( A 340nm /min, NADH[nM]/min, and ∆NADH[nmol]/min) as a function of lactate concentration. 4. (5pts) Present a graph of NADH[nmole]/min as a function of lactate concentration (Michaelis-Menten plot).
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