Lab 3 Data Tables

Direction: Answer thoroughly and explain.

Result nad Discussion    Lead Acetate Reaction: Samples: lysine, cysteine, methionine Reagents: 10% Sodium Hydroxide (NaOH) and Lead Acetate Pb(CH3COO)2 -To 1 ml of the amino acid solution taken in a test tube, add few drops of sodium hydroxide (40%) and boil the contents for 5-10 mins over a bunsen burner. Cool the contents and add few drops of 10% Lead acetate solution and observe.

Materials - 2021FA-CHM-1O X Bb 4427577 d-fleet02-xythos.content.blackboardcdn.com/6086c260d7e8f/4427577?X-Blackboard-Expiration=1633143600000&X-Blackboard-Sig 6 / 9 100% Exercise 1: Standard Curve for Protein Measurements: A standard curve for protein concentration is often created using known concentrations of bovine serum albumin (protein). This process is called the Bradford Assay; it is a colorimetric assay. A special reagent turns blue when it binds to amino acids present in protein. The intensity of the color is best measured with a spectrophotometer (a device for comparing two light radiations, wavelength by wavelength). In the case of the Bradford Assay the greater the absorbance, the higher the protein concentration. A series of tests were performed on some samples and spectrophotometer: following measurements were obtained using a Protein Concentration (mg/ml) Absorbance (A) 0.26 0.098 0.56 0.213 0.383 0.84 1.12 0.473 1.40 0.527 TASKS: 1. Enter the data into Excel - the…

how to calculate the experiment fow rate for this gel chromatography Experiment flow rate ? Flow rate 4.75mL/min

Question: Determine the amount of dehydrated medium needed to prepare 15 butts, 20 slants, and 25 butt-slants. Include amount for 2 additional units of each as excess to compensate for compounding losses.

topic: Isoelectric Precipitation   Appearance of the mixtures containing casein mixed with different buffer solutions: (a) TestTube 1 containing Glycine-HCl buffer; (b) Test Tube 2 containing Acetate buffer; and (c) Test Tube 3containing Phosphate buffer. QUESTION: Based on the given results, what do you think is the isoelectric pH of casein? Briefly discuss your basis for determining it.

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Phenol Red Test Question: Assuming you tested an organism using Phenol Red glucose and Phenol Red Base broths, which of the combinations of results in the following table would be reliable. Interpret each of the combinations and explain how the results might be obtained

Answer number 2

III. Problem solving. Show your computations. Answer questions briefly. A 50.0 ml juice extract is colorimetrically assayed using Nelson's test. One milliliter (1.00 mL) of the solution and the standard glucose solution (concentration: 1mg/mL) were treated with freshly prepared Nelson's reagent and arsenomolybdate reagent and then diluted to 10.0 mL separately in properly labeled test tubes. Absorbance at 480 nm for the standard is 1.702 and for the sample, 0.926. A. What is the principle behind the assay?

Experiment: Animal Oxidase Procedures are indicated below. (Please view the image) Question: What will be the possible result and observation? What's the principle behind the results?

ELISA test for a patient with UTI. Analyse the results attached. Thank you!!!

6,7,8,9

Biomaterial question: Give a brief and clear answer, please a. Briefly describe the structural differences between the three major types of biomaterials,  including their chemical structure, bonding characteristics, crystal structures, etc.  b. Explain briefly the working principle and the information provided by the spectroscopic  and chromatographic characterization techniques . Give an example to each of  them.

Rf Sample values 0.21 В 0.39 C 0.33 0.27 Four amino acid samples were subjected to a normal-phase chromatography set up yielded the following Rf values. Which among the samples is the least polar? E and O A O B OD

INPUT AND OUTPUT COMPUTATION

Biomaterial question: Give a brief and clear answer, please do not write by hand Question: Explain briefly the working principle and the information provided by the spectroscopic and chromatographic characterization techniques. Give an example to each of them.

Question attached

16. Your Excel data graph for Serial Dilution lab:** 16a. Include a clear and labeled graph of DF (x axis) versus Absorbance (y axis). See box above for expectations and review video tutorial of this Excel work. Do not graph two of your data points: your blank or your Cuvette #1 (DF=1). 1 Q A Multiple Styles W S X 3 E D $ 4 To C R F % 5 I T V U ^ 6 G Y 7 H U 8 J BN S ( 9 K M X

Exercise 2: Effect of Temperature on Enzyme Activity_ Samples of starch will be incubated at 3 separate temperatures (0, 37, & 100 degrees C) prior to the addition of the enzyme. Once the enzyme is added, the test tube will be returned to the appropriate temperature environment and the timer will start. Samples will be taken every 15 seconds for the first minute, then at 5 minutes and again every 5 minutes thereafter. The exercises for temperature effects were performed in our labs, one temperature test at a time. Results are shown below: I

Please help as much as possible with with finishing table/questions

How do I compute for this?

pls list 3 electrochemical biosensing topics and give a little summary   not a writing assignment

Pleaseee help asap

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Size Size Crude Anion Cation 2) Exclusion Exclusion Lysate Exchange Exchange 1 2 Total Protein Concentration 15.2 6.6 2 3.75 4.7 (mg/mL) Final Sample Volume 60 30 20 4 3 (mL) Enzyme Specific Activity 0.43 1.7 7.25 18.3 12.5 (units/mgprotein) Based on your protein purification sample data, in which of the purification steps in your protocol did you effectively purify your enzyme? Select all that apply. a) anion exchange correct b) cation exchange correct c) size exclusion 1 correct d) size exclusion 2

Lab 2 Worksheet (cont.) Practice with Solution Calculations How many grams are needed to make each of the following stock solutions (use formula weights on chemical bottles)? 1. a. b. C. d. 100 mL of 0.5 M NaCl (sodium chloride) Mass 0.SX 0₁1 XS = N mass/Molar N = 2.99 nad 100 mL of 0.75 M Na2CO3 (sodium carbonate) 400 mL of 3 M CaCl2 (calcium chloride, anhydrous) 220 mL of 2 M NaHCO3 (sodium biocarbonate) 400 mL of 30% NaNO3 (sodium nitrate) Mos Volume in 'L' +0.5m/519 400 54 1000