Chapter 19 - Oxidative Phosphorylation

pdf

School

University of Toronto, Toronto School of Theology *

*We aren’t endorsed by this school

Course

BLG144

Subject

Biology

Date

Dec 6, 2023

Type

pdf

Pages

Uploaded by MinisterScienceFly31

Chapter 19a Oxidative Phosphorylation and Photophosphorylation Multiple Choice Questions 1. Electron-transfer reactions in mitochondria Page: 707 Difficulty: 1 Ans: E Almost all of the oxygen (O,) one consumes in breathing is converted to: A) acetyl-CoA. B) carbon dioxide (CO,). C) carbon monoxide and then to carbon dioxide. D) none of the above. E) water. 2. Electron-transfer reactions in mitochondria Pages: 712-713 Difficulty: 2 Ans: B Antimycin A blocks electron transfer between cytochromes b and c1. If intact mitochondria were incubated with antimycin A, excess NADH, and an adequate supply of O, which of the following would be found in the oxidized state? A) Coenzyme Q B) Cytochrome a3 C) Cytochrome b D) Cytochrome e E) Cytochrome f 3. Electron-transfer reactions in mitochondria Pages: 715-716 Difficulty: 2 Ans: A In the reoxidation of QH, by purified ubiquinone-cytochrome c reductase (Complex III) from heart muscle, the overall stoichiometry of the reaction requires 2 mol of cytochrome c per mole of QH, because: A) cytochrome c is a one-electron acceptor, whereas QH is a two-electron donor. B) cytochrome c is a two-electron acceptor, whereas QHs, is a one-electron donor. C) cytochrome c is water soluble and operates between the inner and outer mitochondrial membranes D) heart muscle has a high rate of oxidative metabolism, and therefore requires twice as much cytochrome c as QH, for electron transfer to proceed normally. E) two molecules of cytochrome ¢ must first combine physically before they are catalytically active. 4. ATP synthesis

Page: 712 Difficulty: 3 Ans: A If electron transfer in tightly coupled mitochondria is blocked (with antimycin A) between cytochrome b and cytochrome c1, then: A) B) o] D) E) all ATP synthesis will stop. ATP synthesis will continue, but the P/O ratio will drop to one. electron transfer from NADH will cease, but O, uptake will continue. electron transfer from succinate to O, will continue unabated. energy diverted from the cytochromes will be used to make ATP, and the P/O ratio will rise. ATP synthesis Pages: 722-723 Difficulty: 2 Ans: A ‘Which of the following statements about the chemiosmotic theory is correct? A) B) ®) D) E) Electron transfer in mitochondria is accompanied by an asymmetric release of protons on one side of the inner mitochondrial membrane. It predicts that oxidative phosphorylation can occur even in the absence of an intact inner mitochondrial membrance. The effect of uncoupling reagents is a consequence of their ability to carry electrons through membranes. The membrane ATP synthase has no significant role in the chemiosmotic theory. All of the above are correct. ATP synthesis Pages: 722-723 Difficulty:1 Ans: E ‘Which of the following statements about the chemiosmotic theory is false? A) B) 0 D) E) Electron transfer in mitochondria is accompanied by an asymmetric release of protons on one side of the inner mitochondrial membrane. Energy is conserved as a transmembrane pH gradient. Oxidative phosphorylation cannot occur in membrane-free preparations. The effect of uncoupling reagents is a consequence of their ability to carry protons through membranes. The membrane ATPase, which plays an important role in other hypotheses for energy coupling, has no significant role in the chemiosmotic theory. ATP synthesis Pages: 722-723 Difficulty: 3 Ans: A ‘Upon the addition of an uncoupler (collapses the trans-membrane proton gradient) to a suspension of mitochondria carrying out oxidative phosphorylation linked to the oxidation of malate, all of the following occur except: A) B) oxygen consumption decreases. oxygen consumption increases.

o) D) E) the P/O ratio drops from a value of approximately 2.5 to 0. the proton gradient dissipates. the rate of transport of electrons from NADH to O, becomes maximal. 8. ATP synthesis Page: 724 Difficulty: 1 Ans: C Uncoupling of mitochondrial oxidative phosphorylation: A) B) o] D) E) allows continued mitochondrial ATP formation, but halts O, consumption. halts all mitochondrial metabolism. halts mitochondrial ATP formation, but allows continued O, consumption. slows down the citric acid cycle. slows the conversion of glucose to pyruvate by glycolysis. 9. ATP synthesis Page: 724 Difficulty: 2 Ans: A Oligomycin inhibits ATP synthase while DNP is an uncoupler. Therefore, DNP will: A) B) Q) D) E) allow electron transfer in the presence of oligomycin. allow oxidative phosphorylation in the presence of oligomycin. block electron transfer in the presence of oligomycin. diminish O, consumption in the presence of oligomycin do none of the above. 10. ATP synthesis Page: 724 Difficulty: 1 Ans: D ‘Which of the following statements about energy conservation in the mitochondrion is false? A) B) 9 D) E) Drug that inhibits the ATP synthase will also inhibit the flow of electrons down the chain of carriers. For oxidative phosphorylation to occur, it is essential to have a closed membranous structure with an inside and an outside. The yield of ATP per mole of oxidizable substrate depends on the substrate. Uncouplers (such as dinitrophenol) have exactly the same effect on electron transfer as inhibitors such as cyanide; both block further electron transfer to oxygen. Uncouplers “short circuit” the proton gradient, thereby dissipating the proton motive force as heat. 11. ATP synthesis Page: 725 Difficulty: 2 Ans: E ‘Which of the following is correct concerning the mitochondrial ATP synthase? A) B) It can synthesize ATP after it is extracted from broken mitochondria. It catalyzes the formation of ATP even though the reaction has a large positive deltaG".

Your preview ends here

Eager to read complete document? Join bartleby learn and gain access to the full version

Access to all documents
Unlimited textbook solutions
24/7 expert homework help

C) It consists of F, and F, subunits, which are transmembrane (integral) polypeptides. D) Itis actually an ATPase and only catalyzes the hydrolysis of ATP. E) When it catalyzes the ATP synthesis reaction, the deltaG" is actually close to zero. 12. ATP synthesis Page: 726 Difficulty: 2 Ans: D ‘When the deltaG' of the ATP synthesis reaction is measured on the surface of the ATP synthase enzyme, it is found to be close to zero. This is thought to be due to: A) avery low energy of activation. B) enzyme-induced oxygen exchange. C) stabilization of ADP relative to ATP by enzyme binding. D) stabilization of ATP relative to ADP by enzyme binding. E) none of the above. 13. ATP synthesis Page: 729 Difficulty: 2 Ans: C During oxidative phosphorylation, the proton motive force that is generated by electron transport is used to: A) create a pore in the inner mitochondrial membrane. B) generate the substrates (ADP and Pj) for the ATP synthase. C) induce a conformational change in the ATP synthase. D) oxidize NADH to NAD". E) reduce O, to H,O. 14. Mitochondrial genes: their origin and the effects of mutation Pages: 738-741 Difficulty: 2 Ans: A ‘Which one of the following statements about human mitochondria is true? A) The mitochondrial genome codes for rRNA, tRNA and genes encoding subunits of proteins mediating mitochondrial electron transport. B) Mitochondrial genes are inherited from both maternal and paternal sources. C) rRNA and tRNA are imported from the cytoplasm and used in mitochondrial protein synthesis. D) The mitochondrial genome codes for all proteins found in mitochondria. E) The mitochondrial genome is not subject to mutations. Short Answer Questions 15. Electron-transfer reactions in mitochondria Page: 712 Difficulty: 1 As you read and answer this question, you are (presumably) consuming oxygen. What single reaction accounts for most of your oxygen consumption?

Ans: O, is converted to H,O by electrons from the respiratory chain. The final step is the one catalyzed by cytochrome oxidase (Complex IV). 16. Electron-transfer reactions in mitochondria Page: 712 Difficulty: 3 Show the path of electrons from ubiquinone (Q or coenzyme Q) to oxygen in the mitochondrial respiratory chain. One of the two compounds (Q and O;) has a standard reduction potential (E*) of 0.82 V, and the other, 0.045 V. Which value belongs to each compound? How did you deduce this? Ans: QH, - cytb - cytc; = cytc —» cyt(a+as) - O, E' for O, must be the larger positive value (+0.82) because electron flow occurs spontaneously to the electron acceptor with the more positive E". 17. Electron-transfer reactions in mitochondria Pages: 712-717 Difficulty: 3 Diagram the path of electron flow from NADH to the final electron acceptor during electron transport in mitochondria. For each electron carrier, indicate whether only electrons, or both electrons and protons, are accepted/donated by that carrier. Indicate with an arrow where electrons from succinate oxidation enter the chain of carriers. Ans: NADH (both) - FP (both) — Q (both) — cyt b (e~ only) — cytc: (e~ only) - cytc (e—only) — cyt(a + as) (e~ only) ~ O, (both) Electrons from succinate enter at Q. 18. Electron-transfer reactions in mitochondria Pages: 711, 717 Difficulty: 3 A recently discovered bacterium carries out ATP synthesis coupled to the flow of electrons through a chain of carriers to some electron acceptor. The components of its electron transfer chain differ from those found in mitochondria; they are listed below with their standard reduction potentials. Electron carriers in the newly discovered bacterium: Electrons E* Oxidant Reductant transferred W) NAD*NADH 2-0.32 flavoprotein b (FPb) flavoprotein b 2 -0.62 (oxidized) (reduced) cytochrome c (Fe*") cytochrome c (Fe*) 1 +0.22 Fe-S protein Fe-S protein 2 +0.89 (oxidized) (reduced) flavoprotein a (FPa) flavoprotein a 2 +0.77 (oxidized) (reduced)

(a) Place the electron carriers in the order in which they are most likely to act in carrying electrons. (b) Is it likely that O, (for which E™ = 0.82 V) is the final electron acceptor in this organism? Why or why not? (c) How would you calculate the maximum number of ATP molecules that could theoretically be synthesized, under standard conditions, per pair of electrons transfered through this chain of carriers? (The Faraday constant, F, is 96.48 kJ/V'mol.) ~ G™ for ATP synthesis is +30.5 kJ/mol. Ans: (a) FP, NAD" - cytc - FP, - Fe-S (b) No; Fe-S has a larger E", so will probably be the terminal acceptor. (c) First calculate deltaG™ for e~ flow from FP; to Fe-S: deltaE™ = E™(oxidant) — E*(reductant) = +0.89 — (-0.62) = +1.51 V' deltaG™ = —nFdeltaE"™ = (~2)(96.48 kJ/V-mol)(1.51 V) = 291 kJ/2e~ Theoretically, the flow of two electrons from FPb to Fe-S could drive the synthesis of 291 kJ/30.5 kJ/mol = 9.5 mol ATP. Because only whole numbers of molecules can be made, the correct answer is 9 mol ATP per electron pair. 19. Electron-transfer reactions in mitochondria Page: 717 Difficulty: 2 During electron transfer through the mitochondrial respiratory chain, the overall reaction is: NADH + 1/2 0, + H - NAD" + H,O. The difference in reduction potentials for the two half-reactions (deltak™) is +1.14 V. Show how you would calculate the standard free-energy change, deltaG™, for the reaction as written above. (The Faraday constant, F, is 96.48 kJ/V-mol.) Ans: deltaG" = —nFdeltaE" = (-2)(96.48 kJ/V-mol)(1.14V) = -220 kJ/mol 20. Electron-transfer reactions in mitochondria Page: 717 Difficulty: 3 The standard reduction potential for ubiquinone (Q or coenzyme Q) is 0.045 V, and the standard reduction potential (E') for FAD is —0.219 V. Using these values, show that the oxidation of FADH, by ubiquinone theoretically liberates enough energy to drive the synthesis of ATP. The Faraday constant, F, is 96.48 kJ/V-mol. deltaG™ for ATP synthesis is +30.5 kJ/mol. Ans: deltaE™ = E"(oxidant) — E"(reductant) = 0,045 — (—0.219) = +0.264 V deltaG™ = —n FdeltaE"™ = (~2)(96.48 kJ/V-mol)(0.264 V) = —51.0 ki/mol. Passage of two electrons from FADHS, to Q can, in principle, provide sufficient energy for synthesizing 1 ATP. 21. ATP synthesis Pages: 723-724 Difficulty: 2 Describe, in simple diagrams and a few words, the chemiosmotic theory for coupling oxidation to phosphorylation in mitochondria.

Your preview ends here

Eager to read complete document? Join bartleby learn and gain access to the full version

Access to all documents
Unlimited textbook solutions
24/7 expert homework help

Ans: There are three central elements in the chemiosmotic model: (1) Electron flow through asymmetrically arranged membrane-bound carriers causes transmembrane flow of H', creating a proton gradient (a proton motive force). (2) The proton motive force drives protons back across the membrane via specific proton channels (composed of Fo). (3) The energy released by “downhill” movement of protons is captured when ADP and Pi are condensed by ATP synthase (FoF1). (See Fig. 19-19, p. 723.) 22. ATP synthesis Pages: 723-725 Difficulty: 3 Compound X is an inhibitor of mitochondrial ATP synthesis. It was observed that when compound X was added to cells, the NAD*/NADH ratio decreased. Would you expect X to be an uncoupling agent or an inhibitor of respiratory electron transfer? Explain in 30 words or less. Ans: It is an inhibitor of electron transfer; its addition lowers the NAD/NADH ratio because NADH produced by oxidative reactions in mitochondria can no longer be reoxidized by electron flow to O.. 23. ATP synthesis Pages: 723-725 Difficulty: 1 Give an example of (a) an uncoupler of oxidative phosphorylation, and (b) an inhibitor of respiration. (c) Describe the difference in the effects of such uncouplers and inhibitors on mitochondrial function. Ans: (a) Uncouplers include DNP, valinomycin, and CCCP. (b) Respiration inhibitors include antimycin A, piericidin A, CN—, rotenone, and amytal. (c) Uncouplers stop formation of ATP while allowing electron transfer to continue. Inhibitors of respiration block both electron transfer and phosphorylation. 24. ATP synthesis Pages: 723-725 Difficulty: 2 Mitochondria carrying out oxidative phosphorylation consume oxygen. Explain what happens to this oxygen, and describe the effect of an uncoupling agent such as 2,4- dinitrophenol on the rate of oxygen consumption. Assume there is a sufficient supply of oxidizable substrate, ADP, and Pi. Ans: O, is reduced to H,O by electrons passed through the respiratory chain. Addition of 2,4- dinitrophenol, which uncouples phosphorylation (ATP synthesis) from electron flow, actually stimulates O, consumption slightly by removing the “drag” of ATP synthesis. 25. ATP synthesis Pages: 725-726 Difficulty: 2 ‘When the F1 portion of the ATP synthetase complex is removed from the mitochondrial membrane and studied in solution, it functions as an ATPase. Why does it not function as an

ATP synthetase? Ans: Like all enzymes, the F1 subunit of the ATP synthase catalyzes a reaction in both directions: ADP +Pi 7 ATP + H,O The standard free-energy change (deltaG™) for ATP hydrolysis is —30.5 kJ/mol. With no proton motive force to drive the reaction toward ATP synthesis, the hydrolysis (ATPase activity) occurs spontaneously. 26. ATP synthesis Page: 726 Difficulty: 2 ‘When the deltaG™ of the ATP synthesis reaction is measured on the surface of the ATP synthase enzyme, it was found to be close to zero. Describe briefly why this is so. Ans: The enzyme binds ATP more tightly than ADP thus stabilizing the former (i.e., the product of the synthesis reaction) relative to the latter (i.e., the reactant in the synthesis reaction). 27. ATP synthesis Pages: 726-729 Difficulty: 3 Explain briefly the current model for how the proton motive force that is generated by electron transport is used to drive the ATP synthesis reaction. Ans: The tight binding of ATP by the enzyme stabilizes it and makes the deltaG" of the synthetic reaction more favorable. Once the reaction has occurred, the ATP product must be released from the enzyme. The proton motive force causes protons to move across the inner mitochondrial membrane through the pore in the Fo complex. This movement leads to conformational changes that decrease the affinity of the F1 portion of the synthase for ATP, resulting in its release from the enzyme. 28. Mitochondrial genes: their origin and the effects of mutation Page: 739 Difficulty: 2 Discuss three lines of evidence that support the theory that mitochondria evolved from endosymbiontic bacteria. Ans: 1) Modern prokaryotes have respiratory chains very similar to those in mitochondria. 2) Bacteria translate protons outward across their plasma membrane and use this proton gradient to drive ATP synthesis and other energy-requiring processes such as flagellar motion. 3) Unlike most other organelles, mitochondria in eukaryotic cells retain their own genome.

Chapter 19 - Oxidative Phosphorylation

Related Documents