Chapter 19 - Oxidative Phosphorylation

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University of Toronto, Toronto School of Theology *

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BLG144

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Biology

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Dec 6, 2023

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pdf

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Chapter 19a Oxidative Phosphorylation and Photophosphorylation Multiple Choice Questions 1. Electron-transfer reactions in mitochondria Page: 707 Difficulty: 1 Ans: E Almost all of the oxygen (O,) one consumes in breathing is converted to: A) acetyl-CoA. B) carbon dioxide (CO,). C) carbon monoxide and then to carbon dioxide. D) none of the above. E) water. 2. Electron-transfer reactions in mitochondria Pages: 712-713 Difficulty: 2 Ans: B Antimycin A blocks electron transfer between cytochromes b and c1. If intact mitochondria were incubated with antimycin A, excess NADH, and an adequate supply of O, which of the following would be found in the oxidized state? A) Coenzyme Q B) Cytochrome a3 C) Cytochrome b D) Cytochrome e E) Cytochrome f 3. Electron-transfer reactions in mitochondria Pages: 715-716 Difficulty: 2 Ans: A In the reoxidation of QH, by purified ubiquinone-cytochrome c reductase (Complex III) from heart muscle, the overall stoichiometry of the reaction requires 2 mol of cytochrome c per mole of QH, because: A) cytochrome c is a one-electron acceptor, whereas QH is a two-electron donor. B) cytochrome c is a two-electron acceptor, whereas QHs, is a one-electron donor. C) cytochrome c is water soluble and operates between the inner and outer mitochondrial membranes D) heart muscle has a high rate of oxidative metabolism, and therefore requires twice as much cytochrome c as QH, for electron transfer to proceed normally. E) two molecules of cytochrome ¢ must first combine physically before they are catalytically active. 4. ATP synthesis
Page: 712 Difficulty: 3 Ans: A If electron transfer in tightly coupled mitochondria is blocked (with antimycin A) between cytochrome b and cytochrome c1, then: A) B) o] D) E) all ATP synthesis will stop. ATP synthesis will continue, but the P/O ratio will drop to one. electron transfer from NADH will cease, but O, uptake will continue. electron transfer from succinate to O, will continue unabated. energy diverted from the cytochromes will be used to make ATP, and the P/O ratio will rise. ATP synthesis Pages: 722-723 Difficulty: 2 Ans: A ‘Which of the following statements about the chemiosmotic theory is correct? A) B) ®) D) E) Electron transfer in mitochondria is accompanied by an asymmetric release of protons on one side of the inner mitochondrial membrane. It predicts that oxidative phosphorylation can occur even in the absence of an intact inner mitochondrial membrance. The effect of uncoupling reagents is a consequence of their ability to carry electrons through membranes. The membrane ATP synthase has no significant role in the chemiosmotic theory. All of the above are correct. ATP synthesis Pages: 722-723 Difficulty:1 Ans: E ‘Which of the following statements about the chemiosmotic theory is false? A) B) 0 D) E) Electron transfer in mitochondria is accompanied by an asymmetric release of protons on one side of the inner mitochondrial membrane. Energy is conserved as a transmembrane pH gradient. Oxidative phosphorylation cannot occur in membrane-free preparations. The effect of uncoupling reagents is a consequence of their ability to carry protons through membranes. The membrane ATPase, which plays an important role in other hypotheses for energy coupling, has no significant role in the chemiosmotic theory. ATP synthesis Pages: 722-723 Difficulty: 3 Ans: A ‘Upon the addition of an uncoupler (collapses the trans-membrane proton gradient) to a suspension of mitochondria carrying out oxidative phosphorylation linked to the oxidation of malate, all of the following occur except: A) B) oxygen consumption decreases. oxygen consumption increases.
o) D) E) the P/O ratio drops from a value of approximately 2.5 to 0. the proton gradient dissipates. the rate of transport of electrons from NADH to O, becomes maximal. 8. ATP synthesis Page: 724 Difficulty: 1 Ans: C Uncoupling of mitochondrial oxidative phosphorylation: A) B) o] D) E) allows continued mitochondrial ATP formation, but halts O, consumption. halts all mitochondrial metabolism. halts mitochondrial ATP formation, but allows continued O, consumption. slows down the citric acid cycle. slows the conversion of glucose to pyruvate by glycolysis. 9. ATP synthesis Page: 724 Difficulty: 2 Ans: A Oligomycin inhibits ATP synthase while DNP is an uncoupler. Therefore, DNP will: A) B) Q) D) E) allow electron transfer in the presence of oligomycin. allow oxidative phosphorylation in the presence of oligomycin. block electron transfer in the presence of oligomycin. diminish O, consumption in the presence of oligomycin do none of the above. 10. ATP synthesis Page: 724 Difficulty: 1 Ans: D ‘Which of the following statements about energy conservation in the mitochondrion is false? A) B) 9 D) E) Drug that inhibits the ATP synthase will also inhibit the flow of electrons down the chain of carriers. For oxidative phosphorylation to occur, it is essential to have a closed membranous structure with an inside and an outside. The yield of ATP per mole of oxidizable substrate depends on the substrate. Uncouplers (such as dinitrophenol) have exactly the same effect on electron transfer as inhibitors such as cyanide; both block further electron transfer to oxygen. Uncouplers “short circuit” the proton gradient, thereby dissipating the proton motive force as heat. 11. ATP synthesis Page: 725 Difficulty: 2 Ans: E ‘Which of the following is correct concerning the mitochondrial ATP synthase? A) B) It can synthesize ATP after it is extracted from broken mitochondria. It catalyzes the formation of ATP even though the reaction has a large positive deltaG".
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