Worksheet7-Protein

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Pennsylvania State University *

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230

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Biology

Date

Dec 6, 2023

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docx

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1

Uploaded by DeanCrown15929

BIOL230W Week 4 –Translation Worksheet 7 – Protein Structure and Function Figure 1. (A) Domain diagram of RecQ helicases from several organisms. These proteins function in DNA repair. The Zn-binding motif (brown), unique to the RecQ family, is depicted in the C-terminus of the ATPase domain. Most RecQs also share the HRDC ( h elicase-and- r ibonuclease D / C -terminal) domain at the C-terminus, although it is not essential for helicase activity. A nuclear localization signal is depicted as a shaded bar. (B) Structure of WRN RQC domain (blue, ribbon model) bound to the 14-base-pair DNA molecule (orange, tubular model). The secondary and supersecondary structures are labeled. Side chains of the key interacting amino acids are shown as stick models. (C) A view following 90° rotation along the y- axis. 1. What protein family is represented in this figure? What organisms are being compared? Recq helicases. Ecoli, human yeat 2. In Fig 1(A), what do the “boxed” structures represent? What do the thinner lines represent? 3. Does the E. coli protein have more or less domains compared to the WRN human? Why is this evolutionarily important? less 4. What hierarchy of structure is represented in Fig1 (B) and (C)? 5. Does helicase activity require energy (*hint, look at domain functions)? Why or why not? 6. Are there any supersecondary structures represented in the figure? How do these generally differ from domains? Yes, beta wings 7. What secondary structures (sheets, helices or both) appear to lead to the association of WRN with one of the grooves of DNA? (*hint – what part of the domain appears to be interacting with the nucleobases?)
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