Worksheet7-Protein
.docx
keyboard_arrow_up
School
Pennsylvania State University *
*We aren’t endorsed by this school
Course
230
Subject
Biology
Date
Dec 6, 2023
Type
docx
Pages
1
Uploaded by DeanCrown15929
BIOL230W Week 4 –Translation
Worksheet 7 – Protein Structure and Function
Figure
1.
(A) Domain diagram of
RecQ helicases from several
organisms.
These proteins function in
DNA repair.
The Zn-binding motif
(brown), unique to the RecQ family, is
depicted in the C-terminus of the
ATPase domain.
Most RecQs also
share the HRDC (
h
elicase-and-
r
ibonuclease
D
/
C
-terminal) domain at
the C-terminus, although it is not
essential for helicase activity. A
nuclear localization signal is depicted
as a shaded bar. (B) Structure of WRN
RQC domain (blue, ribbon model)
bound to the 14-base-pair DNA
molecule (orange, tubular model). The
secondary and supersecondary
structures are labeled. Side chains of
the key interacting amino acids are
shown as stick models. (C) A view
following 90° rotation along the y-
axis.
1.
What protein family is represented in this figure?
What organisms are being compared?
Recq helicases. Ecoli, human yeat
2.
In Fig 1(A), what do the “boxed” structures represent?
What do the thinner lines represent?
3. Does the E. coli protein have
more or less
domains compared to the WRN human?
Why is this
evolutionarily important?
less
4.
What hierarchy of structure is represented in Fig1 (B) and (C)?
5.
Does helicase activity require energy (*hint, look at domain functions)?
Why or why not?
6.
Are there any supersecondary structures represented in the figure?
How do these generally differ from
domains?
Yes, beta wings
7.
What secondary structures (sheets, helices or both) appear to lead to the association of WRN with one of the
grooves of DNA? (*hint – what part of the domain appears to be interacting with the nucleobases?)
Discover more documents: Sign up today!
Unlock a world of knowledge! Explore tailored content for a richer learning experience. Here's what you'll get:
- Access to all documents
- Unlimited textbook solutions
- 24/7 expert homework help
Related Questions
i struggled with my homework and i need help
arrow_forward
8:52
Protein 1-10092015113603.pdf
https:api.schoology.comv1attachment169963839...
Name
Class
Date
Section Protein Synthesis
pages 148-153)
7-3
SECTION REVIEW
In this section you studied the process of pro-
tein synthesis. You learned that the informa-
tion that DNA transfers to messenger RNA
(MRNA) is in the form of a code. When the
information is decoded, chains of amino acids,
called polypeptides, are formed. Polypeptides During translation, each MRNA codon in turn
make up proteins, which direct biochemical
pathways and are responsible for cell structure
and movement.
The genetic code is determined by the
arrangement of the nitrogenous bases in DNA
and RNA. A code word in DNA consists of a
group of three nucleotides. When transcribed
into MRNA, each code word, or codon, desig-
nates a specific amino acid that is to be placed
in the polypeptide chain. More than one codon
may code for a particular amíno acid. The
MANA sequence AUG serves as an initiator,
or "start," codon. Three other…
arrow_forward
RNA Transcription, Translation, and Mutation
Worksheet
First, here is a strand of DNA. This strand contains both a gene and its promoter region. Circle
the promoter region in blue, draw a yellow box around the TATA box, draw a green box around
the start codon, and draw a red box around the stop codon:
TATATATATTACGTTGCATACGCTCAACGGTCGAAACTGCATGGGCAC
ATATATATAATGCAACGTATGCGAGTTGCCAGCTTTGACGTACCCG
Now imagine this gene has been transcribed into RNA. What would that RNA strand look like?
Before the above RNA strand can be translated, a few modifications must first take place (in
eukaryotes). What are they?
1)
2)
3)
Using a codon chart of your choice (one can be found here, or here) translate the above RNA
transcript (assume no splicing took place). Write the three letter abbreviations for the amino
acids in the image below:
Now imagine that a mutation took place in the original strand of DNA (marked in red)
TATATATATTACGTTGCATACCCTCAACGGTCGAAACTGCATG…
arrow_forward
B Relates to the ribosome codon question please do ii ribosome coding questions
arrow_forward
22
arrow_forward
Please answer the following question, thank you!
arrow_forward
uestion 12 of 30 >
Attempt 2
5'-GGU-3',5'-AAG-3',5'-IAC-3',5'-UGU-3', and 5'-CAA-3'. Give all
A series of tRNAs have the anticodons,
possible codons with which each tRNA can pair.
Consider the wobble rules listed in the table.
First position of anticodon
C
G
A
U
I
Third position of codon
G
U or C
U
A or G
A, U, or C
5'-GGU-3'
5'-AAG-3'
5'-IAC-3'
5'-UGU-3'
5'-CAA-3'
3'-UUU-5'
3'-GUU-5'
3'-UUC-5'
3'-GUG-5'
3'-UUG-5'
Answer Bank
3'-ACA-5'
3'-AAA-5'
3'-CUG-5'
3'-GCA-5'
3'-CCA-5'
3'-ACG-5'
3'-UGU-5'
3'-CCC-5'
3'-UCA-5'
3'-AUG-5'
arrow_forward
This answer is incorrect pls provide the right answer and explanation
arrow_forward
15
arrow_forward
Complete number five..
please explain
arrow_forward
I need help with #28
And #34 thank you
arrow_forward
15-2. Explain whether the following statements are true or false.Justify the false ones.A. Ribosomes are the cytoplasmic structures that duringProtein synthesis is linked by an mRNA moleculeforming polyribosomes.B. The Leu-His-Arg-Leu-Asp-Ala-Gln-Ser- Amino Acid SequenceLys-Leu-Ser-Ser is a signal sequence that directs proteinsinto the endoplasmic reticulum.C. All transport vesicles in the cell must have av-SNARE protein in its membrane.D. The transport vesicles carry protein and lipid to thecell surface.E. If the delivery or distribution of lysosomal proteinsprospects from the trans-Glogii network to endosomes areblock, lysosomal proteins would be secreted by pathwaysof constitutive secretion like those in Figure 15-28.F. Lysosomes digest only substances that have been takenby cells by endocytosis.
15.3 Many of the proteins produced in the reticulumendoplasmic changes such as the addition ofcarbohydrates (oligosaccharides). What is the function of thesesugars in such proteins?
15.4…
arrow_forward
Please help asap encoded text form please not handwritten thank you
arrow_forward
9
arrow_forward
4
arrow_forward
FALSE
Unanswered
a Save
58
What segments of the pre mRNA comprise the coding region?
pre-mRNA
5' UTR
Exon
Intron
Exon
Intron
Exon
3' UTR
MRNA
Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer.
a
5'UTR-EXONS-INTRONS 3'UTR
5'UTR-EXONS-3'UTR
5'UTR-INTRONS-3'UTR
P.
EXONS
a Save
Unanswered
59
What is b?
3'
arrow_forward
Choose the correct option for following three mcqs
7.The first step in the capping reaction is catalyzed by:
Phosphatase
Guanylyl transferase
Methyl transferase
Kinase
None of the above
8.Which of the following statements about tRNAs is FALSE?
They contain an anti-codon loop
They contain several modified bases
Their secondary structure looks like a cloverleaf
They are generally less then 100 nucleotides in length
They are synthesized by RNA polymerase-II
9.During protein synthesis _____ delivers the charged tRNA to the A-site.
EF-Tu
EF-G
eIF-2
RF1
RF2
arrow_forward
helpp, if u could do this one first please
arrow_forward
Variant 1
Task 1
One single polypeptide chain (120 amino acid residues) is produced for protein A in prokaryotic cell. N-terminal amino acid is alanine in the chain of this protein. How many moles of ATP and GTP will be in use for this polypeptide chain synthesis? What post-translational modifications may be for this protein?
Task 2
It is known that ribozymes may be participators of translation pathway. Prove this fact.
arrow_forward
Do not use Ai
arrow_forward
Thank you, I need answers here.
arrow_forward
Answer codon usage based on this description:
You have isolated a new eukaryotic microorganism and want to determine the genetic code of its
mitochondria. Here are the amino acids encoded after translation of two synthetic mRNAs using
mitochondrial extracts from your eukaryote- in this experiment translation can start at any sequence position:
RNA:
Protein:
Tyr-Met-Tyr-Met-Tyr-Met----
UAUAUAUAUAUAUAU---
UAAUAAUAAUAAUAA---
Asn-Asn-Asn-Asn-Asn----
Met-Met-Met-Met-Met----
Based on the results shown here, what can you conclude about the codons in this eukaryote?
A.
B.
C.
D.
O A
O C
OD
OB
AUA
Tyr
Met
Tyr
Met
UAU
Met
Tyr
Met
Tyr
AAU
Met
Trp
Asn
UAA
Asn
Asn
Asn
Asn, STOP or Met Asn, STOP or Met
arrow_forward
You continue to study the expression of the hexose kinase gene and capture the following electron
micrograph of the gene being expressed.
MRNA
1
20
ORI
40
60
TTCGAGCTCTCGTCGTCGAGATACGCGATGATATTACTGGIAATATĞGGGATGCACTATC
5'
3'
AAGCTCGAGAGCAGCAGCTCTATGCGCTACTATAATGACCA'NTATAÇCCCTACGTGATAG
CACTATC
promoter
RNA
polymerase
ribosome
arrow_forward
Which statements are true? Explain why or why not.1 The consequences of errors in transcription areless severe than those of errors in DNA replication.2 Since introns are largely genetic “junk,” they do nothave to be removed precisely from the primary transcriptduring RNA splicing.3 Wobble pairing occurs between the first positionin the codon and the third position in the anticodon.4 During protein synthesis, the thermodynamics ofbase-pairing between tRNAs and mRNAs sets the upperlimit for the accuracy with which protein molecules aremade.5 Protein enzymes are thought to greatly outnum-ber ribozymes in modern cells because they can catalyzea much greater variety of reactions and all of them havefaster rates than any ribozyme.
arrow_forward
34) What amino acid sequence is coded for by the following DNA coding strand?
(Recall: the DNA template strand runs 5' to 3' and the mRNA strand runs
antiparallel to the DNA template strand. Recall that DNA is translated with a
start codon.)
5'-TTATGCGACCAGACCAGTTT-3' Coding strand
arrow_forward
SEE MORE QUESTIONS
Recommended textbooks for you
Biochemistry
Biochemistry
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Cengage Learning
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Related Questions
- i struggled with my homework and i need helparrow_forward8:52 Protein 1-10092015113603.pdf https:api.schoology.comv1attachment169963839... Name Class Date Section Protein Synthesis pages 148-153) 7-3 SECTION REVIEW In this section you studied the process of pro- tein synthesis. You learned that the informa- tion that DNA transfers to messenger RNA (MRNA) is in the form of a code. When the information is decoded, chains of amino acids, called polypeptides, are formed. Polypeptides During translation, each MRNA codon in turn make up proteins, which direct biochemical pathways and are responsible for cell structure and movement. The genetic code is determined by the arrangement of the nitrogenous bases in DNA and RNA. A code word in DNA consists of a group of three nucleotides. When transcribed into MRNA, each code word, or codon, desig- nates a specific amino acid that is to be placed in the polypeptide chain. More than one codon may code for a particular amíno acid. The MANA sequence AUG serves as an initiator, or "start," codon. Three other…arrow_forwardRNA Transcription, Translation, and Mutation Worksheet First, here is a strand of DNA. This strand contains both a gene and its promoter region. Circle the promoter region in blue, draw a yellow box around the TATA box, draw a green box around the start codon, and draw a red box around the stop codon: TATATATATTACGTTGCATACGCTCAACGGTCGAAACTGCATGGGCAC ATATATATAATGCAACGTATGCGAGTTGCCAGCTTTGACGTACCCG Now imagine this gene has been transcribed into RNA. What would that RNA strand look like? Before the above RNA strand can be translated, a few modifications must first take place (in eukaryotes). What are they? 1) 2) 3) Using a codon chart of your choice (one can be found here, or here) translate the above RNA transcript (assume no splicing took place). Write the three letter abbreviations for the amino acids in the image below: Now imagine that a mutation took place in the original strand of DNA (marked in red) TATATATATTACGTTGCATACCCTCAACGGTCGAAACTGCATG…arrow_forward
- uestion 12 of 30 > Attempt 2 5'-GGU-3',5'-AAG-3',5'-IAC-3',5'-UGU-3', and 5'-CAA-3'. Give all A series of tRNAs have the anticodons, possible codons with which each tRNA can pair. Consider the wobble rules listed in the table. First position of anticodon C G A U I Third position of codon G U or C U A or G A, U, or C 5'-GGU-3' 5'-AAG-3' 5'-IAC-3' 5'-UGU-3' 5'-CAA-3' 3'-UUU-5' 3'-GUU-5' 3'-UUC-5' 3'-GUG-5' 3'-UUG-5' Answer Bank 3'-ACA-5' 3'-AAA-5' 3'-CUG-5' 3'-GCA-5' 3'-CCA-5' 3'-ACG-5' 3'-UGU-5' 3'-CCC-5' 3'-UCA-5' 3'-AUG-5'arrow_forwardThis answer is incorrect pls provide the right answer and explanationarrow_forward15arrow_forward
- Complete number five.. please explainarrow_forwardI need help with #28 And #34 thank youarrow_forward15-2. Explain whether the following statements are true or false.Justify the false ones.A. Ribosomes are the cytoplasmic structures that duringProtein synthesis is linked by an mRNA moleculeforming polyribosomes.B. The Leu-His-Arg-Leu-Asp-Ala-Gln-Ser- Amino Acid SequenceLys-Leu-Ser-Ser is a signal sequence that directs proteinsinto the endoplasmic reticulum.C. All transport vesicles in the cell must have av-SNARE protein in its membrane.D. The transport vesicles carry protein and lipid to thecell surface.E. If the delivery or distribution of lysosomal proteinsprospects from the trans-Glogii network to endosomes areblock, lysosomal proteins would be secreted by pathwaysof constitutive secretion like those in Figure 15-28.F. Lysosomes digest only substances that have been takenby cells by endocytosis. 15.3 Many of the proteins produced in the reticulumendoplasmic changes such as the addition ofcarbohydrates (oligosaccharides). What is the function of thesesugars in such proteins? 15.4…arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
- BiochemistryBiochemistryISBN:9781305577206Author:Reginald H. Garrett, Charles M. GrishamPublisher:Cengage LearningBiology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStax
Biochemistry
Biochemistry
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Cengage Learning
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax