Midterm2Fall2023_practice

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Biology

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Dec 6, 2023

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Practice questions for BIO2C03 Genetics – Fall, 2023 1. Which of the following statement is not true about eukaryote genomes? a. The nuclear genome is composed of multiple chromosomes b. The more related two species are, the more similar their genome structures c. The more related two species are, the more similar their DNA sequences d. Individuals within a species always have identical chromosome numbers e. Sister species can have identical gene orders on all chromosomes 2. Which of the following is a correct interpretation of a Giemsa-stained chromosomal band called 3p2.1.32 in humans? a. It’s located on the third smallest chromosome. b. It’s located on the long arm c. It’s closer to the centromere than chromosomal band 3p2.1.33. d. It’s homologous to chromosomal band 3q2.1.32. e. None of the above is correct 3. Which of the following statement about ploidy change is not true? a. Nondisjunction can lead to ploidy change b. All polyploid plants have similar fertility c. Allopolyploids have more intragenomic variation than autopolyploids. d. Wild strawberries are diploid. e. Common bread wheat is a hexaploid. 4. Which of the following statement about chromosome structure change is not true? a. Chromosomal deletions are classified into terminal and interstitial deletions. b. An interstitial deletion of chromosome 11 leads to WAGR syndrome c. A terminal deletion of 5p1.5.2 and 5p1.5.3 leads to cri-du-chat syndrome d. Unequal crossover leads to terminal deletion e. Crossover within a pericentric inverted region cause 50% of gametes being inviable. 5. Chromosomal DNA packaging is a multistep process. Which of the following represents the correct order of packing, from the smallest to the largest? a. Solenoid -> nucleosome -> chromosome scaffold -> chromosome arm b. Nucleosome -> solenoid -> chromosome scaffold -> chromosome arm c. Solenoid -> nucleosome -> nucleoid -> chromosome scaffold d. Nucleosome -> nucleoid -> solenoid -> chromosome arm e. Nucleoid -> nucleosome -> solenoid -> chromosome scaffold -> chromosome arm 6. Which of the following is true about base substitutions? a. A to G base substitution is a transversion mutation b. G to C base substitution is a transitional mutation c. A nonsense mutation refers to a nucleotide substitution causing the DNA not recognized by RNA polymerase during transcription d. A missense mutation refers to a nucleotide substitution causing the DNA mistakenly recognized by a different RNA polymerase during transcription e. Transition mutations happen more frequently than transversion mutations 7. Which of the following is true about mutations?
a. Base substitution in wildtype cells occurs at about 1 x 10 -6 per base per DNA replication b. Trinucleotide repeats expand at a rate of about 1 x 10 -9 per DNA replication c. Expansion of trinucleotide CAG repeats at the huntingtin gene is responsible for the Huntington disease d. Mutations in brain cells will be passed to the next generation e. Copy number change of trinucleotide repeats during DNA replication is because the DNA double helix is slippery. 8. Double-stranded breaks are repaired by which of the following mechanism(s)? a. Non-homologous end joining b. Synthesis-dependent strand annealing c. Translesion repair d. Both a and b e. All a, b, and c 9. Which of the following is true of the gametes of a human female who has nondisjunction of her X chromosomes in meiosis I? a. The gametes contain 22, 23, or 24 chromosomes. b. All the gametes contain 24 chromosomes. c. All the gametes contain 23 chromosomes. d. The gametes contain 22 or 24 chromosomes. e. All the gametes contain 22 chromosomes. 10. Assume that a circular plasmid is 4000 base pairs in length and has restriction sites at the following locations: 600, 1000, 2200, and 3800. Following complete digestion, which of the following represent the expected sizes of the restriction fragments? a. 400 bp, 800 bp, 1000 bp, 1800 bp b. 200 bp, 600 bp, 1200 bp, 1600 bp c. 400 bp, 800 bp, 1200 bp, 1600 bp d. 200 bp, 800 bp, 1200 bp, 1800 bp e. 200 bp, 400 bp, 600 bp, 1200 bp, 1800 bp 11. In reverse genetics, which of the following is the correct order in which the experimenter proceeds? a. selection of mutants with a phenotype of interest and then mapping to locate the effected gene(s) b. random bombardment of the DNA with a known mutagen, followed by observation of offspring for newly acquired traits c. screening individuals by PCR/DNA sequencing to associate altered genes with the trait of interest d. identification of a gene of interest followed by screening for mutations in that gene and studies of the phenotypes caused by those mutations. e. None of the above is correct. 12. Which of the following is incorrect about Yamanaka factors? a. They are a group of transcriptional factors b. They can induce the formation of pluripotent stem cells from human somatic cells such as fibroblast cells
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