MicroBio_Exam1

Out of all these tests, which 2 would help further understand this unknown lab. (Gram Stain and Growth Result is already done) Possible Tests (pick 2 that would help know the unknown bacteria) Gram stain morphology & arrangement color and growth characteristics on agar 37C, 30C and 25C (room temp) CAMP test starch hydrolysis test bacitracin susceptibility test glucose, lactose, sucrose, maltose fermentation tests methyl red/MR test Mannitol MacConkey Phenol Red Vogues-Proskauer/VP test citrate utilization test urea hydrolysis test phenylalanine deaminase test SIM test (Sulfide/Indole/Motility) Nitrate reduction test Oxidase test Catalase test

First step : Below I have attached the image of gram stain. -We need to find the name of the compound. And after that , what possible test can be performed to find the compound. We need to do 6 test . So can you please help me with the names for the test to be performed to find the compound. Thank you so much ?

the image contains results of the fluid thioglycolate medium tests. Please explain the results and the chemistry of each tube? Thank you

What additional test would you need to perform to distinguish between the two organisms you have identified?

How do I know what errors would effect my result in gram staining experiments?

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Can you help me with my homework, please? I'm not sure if I got correct some of them on table

Out of the three gram-positive bacteria (Micrococcus luteus, Staphylococcus aureus, and Staphylococcus epidermidis).  Which of the following test(s) will help me identify the unknown gram-positive bacteria?     Gram Stain Wet Mount (motility testing) Endospore Staining Acid Fast Staining Differential & Selective Media (MacConkey, PEA, EMB, MSA Agar, Blood Agar, etc.) Oxidative-Fermentative Test Carbohydrate Fermentation MR-VP Testing Indole Production Citrate Utilization Triple Sugar Iron Testing Catalase Test

Which of the following is/are true regarding the acid-fast stain? (There may be more than one correct answer.) Non-acid-fast microbes appear blue in a completed acid-fast stain. O Acid-fast cells retain the primary dye after treatment with acid-alcohol. O It is used to identify members of the genus Mycobacterium. V Acid-fast cells appear bright pink/red in a completed acid-fast stain. O If cells are acid-fast, they are gram-negative.

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Can you help me with this chart because I struggled with them?

izzes/00071/take/questions/600381 Regarding Gram staining, what will be the appearance of the Gram-positive bacteria if... All steps are done correctly? The slide is not heat-fixed prior to staining? Crystal violet was omitted? lodine was omitted? Only iodine was used? Acetone:alcohol was omitted? Too much acetone:alcohol was used or was left on for too long? I Not enough acetone:alcohol was used? Carbol fuschin or safranin was omitted? W [Choose ] [Choose ] Purple Clear Pink Orange [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] 88 22°C

Questions associated with Gram Stain and KOH Test: Q1) Come up with a step-by-step procedure for the gram stain

In the image attached is a unknown bacteria lab. The gram stain result and growth result is already given to us and is shown. What other tests (1-2 examples) could we use to follow up this test. Explain why we should use those tests with justification.

How does smear preparation of cells from a liquid medium differ from preparation of cells from a solid medium? Mark all that apply: O Water must be added to a smear from liquid media but not from solid O Water must be added to a smear made from solid media but not liquid media A smear made from liquid media will be more thick than a smear made from solid O A smear made from liquid media will be thinner than a smear made from solid media O A smear made from liquid media doesn't have to be fixed but a smear from solid media does Microsoft Bing 11:31 AM 87°F Sunny 9/14/2021

question 6

Given: The used ocular objective while taking this image, has magnifying power of 6x The used objective while taking this image, has a magnifying power of 100x The used stage micrometer has spaces graduation of 01 mm each Ten (10) graduations pn the ocular micrometer conincided with two (2) graduations on the stage micrometer Question: What is the distance of one ocular division? Show your solution.

Please you the first image as an example to fill the gaps on the 2nd image .please no explanation, just fill in the gap . Thanks

Given: The used ocular objective while taking this image, has magnifying power of 6x The used objective while taking this image, has a magnifying power of 100x The used stage micrometer has spaces graduation of 01 mm each Ten (10) graduations pn the ocular micrometer conincided with two (2) graduations on the stage micrometer Question: What is the total magnification of the image under microscope? Show your solution.

Put the following steps for running a gel in order. first Remove comb and put gel int v [ Choose ] Load samples into gel Let gel solidify for 15-20 minutes Connect electrodes and turn on power to run gel second Pour heated, liquid agarose into gel cartridge Remove comb and put gel into bottom of electrophoresis box Pour buffer into electrophoresis box to cover gel third fourth Pour heated, liquid agarose in v fifth Load samples into gel sixth Connect electrodes and turn v

We did the experiment in class but I don't understand the second page which is number 5 can these be explain thank you

A tampered sack of powdered milk was analyzed by getting 50 grams of composited sample and diluting it with 450 ml of sterile distilled water. One ml and 0.1 ml of sample suspension were inoculated in 50 ml Dextrose Tryptone Bromcresol Purple agar and were heated at 100˚C for 5 minutes. Each flask was poured into 3 plates. After the incubation, opaque colonies surrounded by a yellow halo in purple field were counted. The following counts were obtained from the plates: 95, 107, 92, 26, 28 and 27. Compute for the total spore counts and express your final answer per 50 grams of the powdered milk sample.

How does smear preparation of cells from a liquid medium differ from preparation of cells from a solid medium? Mark all that apply:     A smear made from liquid media will be thinner than a smear made from solid media A smear made from solid media doesn't have to be fixed but a smear from liquid media does A smear made from liquid media will be more thick than a smear made from solid Water must be added to a smear made from solid media but not liquid media A smear made from liquid media doesn't have to be fixed but a smear from solid media does Water must be added to a smear from liquid media but not from solid

Is is best to observe slides of bacteria stained with the acid fast stain under high dry magnification. True or false

Complete for Additional Differential Staining B: Negative staining ~ thin smear C: Endospore Stain

The number of bacterial cells in a culture broth is to be determined by a culture technique. Serial dilutions were performed and a 1 mL aliquot from each dilution was mixed with warm molten agar and poured into a Petri dish. The numbers of bacterial colony forming units (CFU) after overnight incubation are shown in the table below. What is the number of colony forming units per mL of the culture broth? Choose only the most appropriate plate for your calculation. Give your answer as the number only (do not add text for the units). You may use scientific notation with the format 1.12e+6 (that is, 1.12 x 106 cfu/mL). (Note: Canvas will then display your answer a whole number.) Plate Dilution Plate 1 10 dilution Plate 2 10 dilution -5 Plate 3 10 dilution Plate 4 10 dilution Plate 5 107 dilution -8 Plate 6 100 dilution *Too many to count Number of colony forming units (CFU) TMTC* TMTC* TMTC* 867 154 18

Please help me with this question urgently please draw the diagram also thanks.