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Directions: The following story is about a crime solved through biotechnological techniques. The techniques used are DNA fingerprinting and polymerase chain reaction (PCR). DNA fingerprinting is a technique in which an individual’s DNA is analyzed to reveal the pattern of particular short nucleotide sequences. This pattern is claimed to be unique to the individual concerned and can thus be used for identification purposes. Polymerase chain reaction, on the other hand, is a technique used to replicate a DNA fragment so as to produce many copies of a particular DNA sequence. Why do you think there’s a need to generate several copies of the DNA? You will learn the answers to this as you go through the story. Read the story carefully and answer the questions that follow.   Questions: 1. How did the investigators conclude that the suspect was indeed the murderer?  2. What if a strand of hair was found in the truck bed instead of a seed pod, do you think it will still lead to the…

Please convert to past tense and passive voice by using point form

Can you please check my answer and make sure it is correct.    Question: How can DNA evidence be used to convict or exonerate a defendant? Why is DNA evidence so powerful?    Answer: DNA evidence can be used to perform DNA profiling to determine the genotype of the specific DNA sample. With just a small amount of DNA, PCR can produce billions of copies of that specific segment. The segments that are used are from non-coding regions that contain STR’s or short tandem repeats. These very short DNA sequences are repeated and are specific to individuals because we inherit them from our mother and father. Gel electrophoresis separates the PCR products based on their size and each band is compared to the allele ladder. This process helps to identify the alleles present in the original samples. DNA profiling is performed at many loci to be able to tell the genetic difference between different individuals with a lot of certainty. The DNA from the different suspects is compared to the allele…

Please support your answer with any source, such as a book or scientific research

Write a brief paragraph about what a scientist could do with DNA. Could you please help me with this? I am struggling tremendously. Thanks!

(THIS IS NOT GRADED.) This is NON-MANDATORY homework for a practice assessment. I need some assistance with these questions, so I can use them for studying material. Thank you so much! I highly appreciate it! (I am using this for study material.)

DNA EXTRACTION Materials: knife                                                               1 cup of fruit                              Table salt                                                       clean piece of cloth or strainer for filtering resealable bag                                              dishwashing liquid 70% rubbing alcohol (chilled)                   shot glass (or any transparent and narrow container resembling a test tube)                                                                                                                           How to do the extraction: Before you begin, make sure you have chilled your alcohol in the freezer for at least 3 hours. Room temperature alcohol will not work nearly as well as cold alcohol. Place about 50 mL of alcohol in the freezer to chill it and take it out only when you’re going to need it. (The alcohol will not actually freeze.)   1. The first thing you will need is a sample. Since DNA is found in all living…

Please provide answers of both parts ..

Please draw or send me a picture of the structure of DNA and label the parts. Thank you very much.

Can you please check my answer and make sure it is correct.   Question: Describe the two roles primers play in PCR.  Answer: Primers are short sequences of single stranded DNA that mark both the ends of the target sequence to be amplified during PCR. One primer attaches to the top strand at one end (forward primer) and the other one attaches to the bottom strand at the other end of the segment (reverse primer). They are made specifically to attach to these sections of DNA because the nucleotides of the primers are complementary to the target sequence. Other than marking the starting and end points for PCR, primers are also necessary for the process because without them, DNA polymerase wouldn’t be able to synthesize the complementary strand of DNA because it can only add nucleotides to an existing piece.

How is the DNA used for catching crime suspects. Describe the procedure and cite particular example where it helped solve a case or absolved an innocent person from any wrongdoing

Copy and paste the link below and watch the video on Youtube and Answer the Questionshttps://www.youtube.com/watch?v=g-dNJdOvBM4 Polymerase Chain Reaction  Questions: 1. What are the materials used for the polymerase chain reaction?  2. Draw a schematic diagram of the procedure in PCR.  3. Why is it important to design the primers at the start of the laboratory procedure? 4. What are the components of the PCR buffer and what is its pH range? What is the purpose of the buffer? 5. What is the use for magnesium chloride?  6. How much template DNA is added? What is the concentration of the primers? 7. At what temperatures does denaturation, annealing and extension occur? Why are the processes placed in that temperature? 8. In this particular PCR experiment, how many cycles was used? 9. Can this PCR be used on its own to find out if a person has Covid or not on its own? Why or why not?

You are working as a CSI analyst. You collected DNA at a crime scene that is from the suspect but do not have enough to run a test to determine who the suspect is. Please discuss what you would do to attain more of the DNA and how to test the DNA to determine who the suspect is.

Please help with this set up! I have tried for a couple hours and I am not getting anywhere. My calculations dont make any sense.

Can you please check my answer and see if it is correct.    Question: In addition to master mix, what else must be added to each PCR tube? Why?    Answer: In addition to master mix, the primers must be added to the PCR tubes. Each tube is also reserved for one individual’s specific section of DNA. So not only do primers needed to be added that are made specifically for the person’s DNA, but the DNA itself from the suspect needs to be added to the PCR tubes (DNA template strand). We also must have a control which contains sterile water, and one PCR tube reserved for DNA from the crime scene.

I need help defining what is DNA Chromatography.

Please provide answers of both part ..

Indicate true or false for the following statements   The glycerol used in the DNA loading dye allows DNA to be visualized under UV light. The DNA Ladder used for agarose gel electrophores can be used to estimate fragment size and DNA concentration. During gel electrophoresis a DNA smear may indicate that DNase was still present in the sample.

DNA Sample : Blood Stain collected from handle of knife blood Bob Sue John Lisa stain 1. What do you notice about the black lines? 2. How do we read the gel electrophoresis? 3. Which sample are we comparing Bob, Sue, John, and Lisa's DNA to? 4. How do they compare?

6. Refer to the figure answer the following questions. Alignment Hide Colors View Alignment File CLUSTAL W (1.83) multiple sequence alignment Human_AA Oyster AA Corn_AA -MKLFWLLFTIGFCWAQYSSN--TOOGRTSIVHLFEWR--------VDIALECERYLAPK 50 -QVILWCLLYVGVVRGGTWSNPTCAPGRHTITHLFEWK- MAKHLAAMCRCSLLVLVLLCLGSQLAQSQVLFQGFNWESWKKOGGWYNYLLGRVDDIAAT 60 --WSDIAAECERFLGPM 52 :.. GFGGVQVSPPNENVAIHNPFRPWWERYQPVSYKLCTRSGNEDEFRNMVTRCNNVGVRIYV 110 Human_AA Oyster AA Corn_AA GYCGVQISPPNENRIVTSPNRPWWERYQPVSYKLVTRSGNEADLRDMVQRCNKVNVRIYA 112 GATHVWLPPPSHSVAPQGYMPGRLYDLD-----ASKYGTHAELKSLTAAFHAKGVKCVA 114 :: *.. :::.:. : .*: *... DAVINHMCGNAVSAGTSSTCGSYFNPGSRDFPAVPYSGWDFNDG-KCKTGSGDIENYNDA 169 DVVINHMTG-AGGSGTG-TGGSHWDGGSLSYPGVPFSSWDFNSGSECSTGDGNIHNYNDP 170 DVVINHRCA---DYKDGRGIYCVFEGG- -TPDSRLDWGPDMICSDDTOYSN--GRG 163 Human_AA Corn_AA :: * Figure: Sequence alignment a) How many different species are used as the source of sequence in this analysis? I. two II. one III. three IV. four b) What does the (*) mark mean in…

The following gel shows the results of a crime scene investigation. Lane 1 shows a DNA sample that was obtained from the scene of a crime (there was evidence of the criminal cutting himself, so it's the criminal's blood sample that produced the DNA bands in lane 1). Lanes 2-7 shows a DNA sample from six potential suspects held in connection with the crime. Arrange the bands labeled A, B, C, D from largest to smallest. 1 2 3 4 5 6 7 OC, A, D, B A, B, C, D OB, D, A, C O D, A, C, B

could I have help with this question?

Complete the table. Answer the red colors.

Please draw the structure of DNA and label the parts. Thank you very much for your help.

A health provider contacts you regarding a specific patient. They ask you to sequence this patient’s DNA and sends you her blood sample. You split the patient’s blood sample into three equal parts. With each part, you perform three different protocols of DNA extraction and purification. You analyze the resulting DNA using the nanodrop. Protocol A260/280 ng/ul DNA Total volume (ul) 1 2.02 50 60 2 1.45 156 200 3 0.30 300 120 d)Halfway through the electrophoresis of your gel you realized that rather than using a buffer to make up your gel, you actually used distilled water. Do you think this mistake would alter the outcome of your gel electrophoresis? Explain. e) If you added your DNA to a well in a gel without first mixing it with gel loading solution, would this impact the final results? Explain. f) Only after the run you realize that you forgot to add ethidium bromide to the gel. What will happen to your sample? What could you do to the gel after the run?

RECOMBINANT DNA BRIEFLY, DESCRIBE RECOMBINANT DNA AND GIVE ONE CONCRETE EXAMPLE. EVALUATE THE SIGNIFICANCE/PRACTICAL APPLICATIONS OF THIS DNA TECHNOLOGY BY CONSIDERING ETHICAL AND MORAL IMPLICATIONS BEHIND IT.  RECOMBINANT DNA   EXAMPLE: MODIFIED TRAIT GENE MODIFICATION RECIPIENT ORGANISM FIELDS OF APPLICATION                 ALSO, WHAT ARE YOUR INSIGHTS? IN 2 TO 3 SENTENCES.

Which of the following are necessary for Sanger sequencing? Select all correct answers. Group of answer choices ddNTPs an oligonucleotide primer DNA polymerase dNTPs DNA ligase a restriction enzyme

Once you have cut your circular DNA it becomes linear and you need to insert/glue the functional gene sequence with the cut piece of DNA. What will you use to glue the pieces of DNA together? Edit View Insert Format Tools Table 12pt v Paragraph v B I UA

The following statements are either accurate as written or contain some errors. You need to rewrite each as an accurate statement. Make sure that you do not change the material the sentence is about. Highlight or underline any changes that you make to statements. Thank you! 1. Primers cleave DNA at recognition sequences. They can produce either matching ends, which are fragments with overhangs, or it can produce blunt ends, which are fragments without overhanging ends.  2. Codons are three nonoverlapping (such that each ribonucleotide is only in one codon) ribonucleotides that specify a particular amino acid. They are "read" in a linear fashion by the ribosome to be translated into a polypeptide.  3. The diagram below is an illustration of an important molecule for transcription. It depicts a ribosome that has two important regions. Region A that attaches to an amino acid and Region B that contains a codon.

Your next task is to study Ebola virus (EboV) sequences of different strains. You have received sequences of different Ebola virus strains collected during the Ebola outbreak of 2013 – 2016.  EboV from Guinea pig Reference DNA Sample  CTA TGC AAG CAG TTA mRNA           Protein

Answer each of the following correctly. Designer Genes Work (This is all about Applications of Recombinant DNA). 1. What is Genetically Modified Organism (GMO)?(1-4 sentences only) 2. Illustrate your own Designer genes using this information:The Arctic apple is a fruit engineered to resist browning after being cut. Currently they are only available in the US – in golden, fuji and gala varieties – where they have been given Food and Drug Administration approval. If approved in Europe, they would have to be labelled as genetically modified. The manufacturers claim the main benefit is to help cut down on food waste. And based on the following: a. Identify a special trait. b. Identify a source organism. c. Identify a target organism d. Identify the modified/added trait. Example: Hot Tomato > Chili > Tomato > Spicy Tomato It was reported this week that Brazilian scientists are hoping to create spicy tomatoes using Crispr gene-editing techniques. Although tomatoes…

Can you please check my answer and make sure it is correct. Question: List the ingredients of master mix, and state the purpose of each ingredient.  Answer: Taq DNA polymerase This enzyme synthesizes the complementary strand of the DNA template after attaching to the primer. This means that it adds on free nucleotides to the existing strand, but helps speed up the covalent bonding between these newly added nucleotides. This enzyme is also thermally stable meaning that it can withstand the hot temperatures needed for PCR to occur. This hot temperature is needed for the denaturation step when the double stranded DNA has to be unwound and separated into two strands.  Individual building blocks of DNA (either free nucleotides A, T, C, and G or dNTP’s) These nucleotides are needed to build the complementary strand of DNA A special buffer to maintain the optimal pH, salts, and MgCl2 These buffers help maintain a good pH that doesn’t become too acidic or basic for Taq DNA polymerase to…

Create a script about genetic manipulation on animals and plants. All contents of the video must be original. Thank you, it's for my project.

Select the correct terms: To work on removing PERV DNA from the pig genome, researchers first replicate the DNA many times, using (PCR / CRISPR). This process increases the amount of DNA so that they are able to run experiments to edit the genome, using (PCR / CRISPR).

Answer each of the following correctly. Designer Genes Work (This is all about Applications of Recombinant DNA). 1. How does DNA Replicate?(1-3 sentences only) 2. What is Genetically Modified Organism (GMO)?(1-4 sentences only) 3. Illustrate your own Designer genes using this information:The Arctic apple is a fruit engineered to resist browning after being cut. Currently they are only available in the US – in golden, fuji and gala varieties – where they have been given Food and Drug Administration approval. If approved in Europe, they would have to be labelled as genetically modified. The manufacturers claim the main benefit is to help cut down on food waste. And based on the following: 1. Identify a special trait. 2. Identify a source organism. 3. Identify a target organism 4. Identify the modified/added trait. Example: Hot Tomato > Chili > Tomato > Spicy Tomato It was reported this week that Brazilian scientists are hoping to create spicy tomatoes using Crispr…