Quantifying the Niche Part II

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Arizona State University *

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182

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Biology

Date

Apr 3, 2024

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pdf

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5

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1 Part II: Quantifying the Niche Writing Assignment Effect of different water temperatures on Daphnia’s heart rate and stress response Brooke Fulkerson Arizona State University Biology 182: General Biology II Kim Michel/ Anagha Devulapalli March 3, 2024
2 Introduction: Daphnias are aquatic creatures that are widely used as model organisms in scientific research. Daphnia’s heart rate and stress response are affected by numerous factors. One of the factors is water temperature. During the investigation, we investigated the effect of different water temperatures on Daphnia's heart rate and stress response. Procedures: To prepare the lab equipment, a 50mL beaker, two square pieces of mesh, pipette, a microscope, and thermometer were used. Filling a beaker with spring water, and catching the Daphnia, then collecting one Daphnia with a pipette and placing it on one of the mesh squares. Placing the second mesh square on top lightly, to prevent the Daphnia from swimming around during observation. We observed the Daphnia through a microscope in order to locate the heart, observing the heart rate for 60 seconds and recording the water temperature and heart rate. The observation was repeated three times with three different Daphnia with water temperatures ranging from 15°C to 40°C. The heart rate and water temperature were recorded for each of the trails to determine the type of relationship that was occurring between the heart rate and stress response under different conditions. The results were interpreted to make conclusions about the Daphnia’s sensitivity to heat and stress response to different temperatures. Data: The following was collected during the experiment Water temperatures Daphnia #1 Daphnia #2 Daphnia #3 Daphnia #4 Average BPM at 15 126 BPM 139 BPM 162 BPM 142 142.5 BPM
3 The heart rate for the average heart rate between the 3 trails at the 6 different temperatures is displayed in the graph. The heart rate varies between each of the different temperatures but BPM at 20 152 BPM 178 BPM 198 BPM 230 BPM 189.5 BPM BPM at 25 280 bpm 190 bpm 210 bpm 253 bpm 233.25 BPM BPM at 30 260 BPM 360 BPM 400 BPM 280 BPM 325 BPM BPM at 35 364 bpm 324 bpm 332 bpm 404 bpm 356 BPM BPM at 40 356 bpm 320 bpm 297 bpm 332 bpm 326 BPM
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