Quantifying the Niche Part II
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Apr 3, 2024
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Part II: Quantifying the Niche Writing Assignment
Effect of different water temperatures on Daphnia’s heart rate and stress response
Brooke Fulkerson
Arizona State University
Biology 182: General Biology II
Kim Michel/ Anagha Devulapalli
March 3, 2024
2
Introduction:
Daphnias are aquatic creatures that are widely used as model organisms in scientific
research. Daphnia’s heart rate and stress response are affected by numerous factors. One of the
factors is water temperature. During the investigation, we investigated the effect of different
water temperatures on Daphnia's heart rate and stress response.
Procedures:
To prepare the lab equipment, a 50mL beaker, two square pieces of mesh, pipette, a microscope,
and thermometer were used. Filling a beaker with spring water, and catching the Daphnia, then
collecting one Daphnia with a pipette and placing it on one of the mesh squares. Placing the
second mesh square on top lightly, to prevent the Daphnia from swimming around during
observation. We observed the Daphnia through a microscope in order to locate the heart,
observing the heart rate for 60 seconds and recording the water temperature and heart rate. The
observation was repeated three times with three different Daphnia with water temperatures
ranging from 15°C to 40°C. The heart rate and water temperature were recorded for each of the
trails to determine the type of relationship that was occurring between the heart rate and stress
response under different conditions. The results were interpreted to make conclusions about the
Daphnia’s sensitivity to heat and stress response to different temperatures.
Data: The following was collected during the experiment
Water
temperatures
Daphnia #1
Daphnia #2
Daphnia #3
Daphnia #4
Average
BPM at 15
C°
126 BPM
139 BPM
162 BPM
142
142.5 BPM
3
The heart rate for the average heart rate between the 3 trails at the 6 different temperatures is
displayed in the graph. The heart rate varies between each of the different temperatures but
BPM at 20
C°
152 BPM
178 BPM
198 BPM
230 BPM
189.5 BPM
BPM at 25
C°
280 bpm
190 bpm
210 bpm
253 bpm
233.25 BPM
BPM at 30
C°
260 BPM
360 BPM
400 BPM
280 BPM
325 BPM
BPM at 35
C°
364 bpm
324 bpm
332 bpm
404 bpm
356 BPM
BPM at 40
C°
356 bpm
320 bpm
297 bpm
332 bpm
326 BPM
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