Culturing bacteria and aseptic technique - Lab 4

On which of the following types of media would you expect untransformed bacteria to grow on? LB agar LB agar with ampicillin Untransformed bacteria will not grow on any type of medium. Untransformed bacteria will grow on all three types of media. LB agar with ampicillin and arabinose

After quick-characterization by staining, specialized growth media can be used to indicate further biochemical characteristics of an unknown organism. These tests use visual outcomes, such as colors, patterns, and changes in appearance. The results can be interpreted to then assign a characteristic to the unknown organism. For example, a color change may happen when an unknown organism is incubated in a mixed sugar broth. The color could indicate which sugar was (or was not) used by the bacterium for energy.   a) explain in your own words how to perform specialized media tests to find an unknown bacteria

method used in lab? we will be using the filter disk method. Which is NOT true of the filter disc A zone of inhibition is an area of no growth around the filter disc. You will apply an agent, such as a particular mouthwash, to the filter disc. You should apply the mouthwash to a plate that has been already been swabbed by the bacteria by pipetting one drop of the mouthwash or rinse directly onto the plate. A zone of inhibition indicates that an agent that has been applied to the filter disc either inhibited or killed the organism on the spread plate.

A growth medium consisting of only known amounts of water, magnesium, calcium chloride, potassium and glucose would be considered a chemically defined medium a complex medium a chemically nondefined medium differential medium selective medium

Could you explain the difference ( and possibly show the difference) between a growth on Agar slants and Growth in broths? Next, could you describe what E. Coli, M. Luteus, and an uninoculated growth should look like on both agar slants and broths? Much thanks! I am a bit confused

Is the mannitol salt agar (MSA) a complex or defined medium? Explain based on Composition. What kind of media based on what kind of microorganisms it allows to grow

a typical labeled sketch of a bacterial growth curve in batch culture

Please help me with this question. The answer choices are in [ ] If bacteria are killed by an antibiotic, the antibiotic is       ["Bactericidal" OR "Bacteriostatic"]  and we would expect       ["Growth" OR "No Growth"]  on our TSA subculture plates If an antibiotic does not kill the bacteria, but rather the bacterial growth is inhibited, the antibiotic is       ["Bactericidal" OR "Bacteriostatic"]  .  For these types of antibiotics, we would expect       ["Growth" OR "No Growth"]  on our TSA subculture plates in this latter type of antibiotic.

What is growth in "pure culture"? Growth of human pathogens Growth of just one species in the lab Growth of bacteria in liquid Exponential phase of growth

A. Bacterial Growth Curve Categorization. Drag and drop the microbial culture plates on the column that best represents the bacterial colony growth phase. -Number of Bacterial Cells (Log) ➡➡➡ Lag Exponential Phase Phase Stationary Phase Time (h) 24 Death Phase 48

Antibiotics should be reduced or eliminated from culture media during cell subculturing. Make it more detailed.

In a bacterial culture on an agar plate, what is a zone of inhibition?     An area where the growth of bacteria is increased compared to the rest of the agar plate     An area where the growth of bacteria is prevented or reduced compared to the rest of the agar plate     An area where the growth of bacteria is not affected compared to the rest of the agar plate     An area where the the agar becomes contaminated with mold

4. (a) Which of the following chemical reactions is an overall oxidation-reduction (redox) reaction that involves oxidation number changes in their reactions? A. NaOH + H₂O → Na¹ + H3O+ + OH B. HCl + H₂O → H3O+ + Cl- C. CO₂ + H₂O → H+ + CO3²- D. N₂ + 3H₂ → 2NH3

MSA (Mannitol Salt Agar) Match the description to the correct Bacterium A= growth, agar is yellow B= Growth, agar is not yellow C= no growth

Of what practical importance are air borne microorganisms to the laboratory workers?  What precautions should be taken to control laboratory contaminants?  Why are petri dishes incubated in an inverted position?  Of what advantage is the using a solid and a liquid medium?

1. Table 1 lists a typical recipe for growing bacteria in the lab. A researcher discovered a potentially new species of bacterium from a soil sample and attempted to grow it in this media. Unfortunately, the bacterium did not grow. Identify at least three components that you suggest adding to the medium to enable growth. Provide a reason for adding each component. Table 1. per 1000 mL 1g Bacteria culture media NazHPO4•7H2O KH2PO4 3g 5 g 1 g 0.4% (wt/vol) 0.2% (wt/vol) NaCl NHẠC1 Glucose Casamino acids

Microbiology

Give explanation

Bacteria are not used as probiotic agents. O True O False The growth of bacteria that form endospores can be controlled by boiling. O True False Submit Back

Why is a Bunsen burner used in the microbiology lab? (select all that apply) The flame is used to sterilize the inoculating loop. The flame kills microorganisms that float in the air and could contminate samples. To sterilize the lid of the agar plate. To reduce the spread of antibiotic resistance.

Bacteria growth and multiplication play a role in infectious diseases : Bacteria can increase in their number by binary fission into two daughter cells. The rate of exponential growth of a bacterial culture is expressed as generation time, how can the growth curve effect on generation time bacterial?

Background: The following results were obtained from a broth dilution test for microbial susceptibility. What is the minimum bactericidal concentration of this antibiotic and explain what you based your answer on?

Bacteria can increase in their number by binary fission into two daughter cells. The rate of exponential growth of a bacterial culture is expressed as generation time, how can the growth curve effect on generation time bacterial؟

Of what practical importance are air borne microorganisms to the laboratory workers?  What precautions should be taken to control laboratory contaminants?  Of what advantage is the using a solid and a liquid medium?

Before viewing a specimen of pigmented bacteria on a slide under the light microscope, which of the following usually needs to happen (and why)?     Staining (to increase magnification).     Heat, radiation or antimicrobial chemical treatment (to kill the bacteria for safe observation).     Add immersion oil (to increase resolution).     Add a cover slip (to reduce contamination).     The viewing chamber needs to be flushed of air (to create a vacuum).

Match the description to the correct phase in a standard bacterial growth curve. best time to perform Gram stains [ Choose ] [Choose ] phase in which one would see the highest concentration of endopores lag phase stationary phase phase in which bacteria are most sensitive to log/exponential stage antibiotics death/decline phase many bacteria ih biofilms are in this phase, [Choose] thus are more resistant to antibiotics stage at which "carrying capacity" is reached [Choose ]

None

There are 10 mL of a culture of a bacteria at 104 cells/mL into 990 mL of the same broth at the same temperature (37° C) It is 8 p.m. the night before your lab which starts at 11 a.m. Doubling time for this species is 40 minutes. Will the culture reach Stationary Phase before your lab? Set up the equation correctly, solve for t, and tell me what time the culture will reach Stationary Phase.   t /g = (Log Nt – Log N0) /0.301

Please label the figure to demonstrate your understanding of selective and differential media. General-purpose Differential Complex medium Selective medium Simple medium medium medium

Need help In a micro lab there are various staining procedures, different microscopes, and media that are used to grow, test, and view microbes. Imagine you are a microbiologist explaining an aspect of the prokaryote and how to work with it in the lab. The options for discussion are endless, its just a matter of tying the two topics together. For example: for an acid fast cell wall requires a specialized stain, and then a microscope to view.

Membrane filters are probably used for sterilization because filters with smallest pore size do not clog easily. many materials are destroyed by heat sterilization they are used to sterilize only very small amounts of liquid they denature proteins

Indicate which of the following observations can be made when observing growth on nutrient agar. Check all that apply. please look at the attached image

10. You have a 20mM stock solution of biofilm inhibitor compound X. You are asked to prepare a test tube with growth media and compound X at a final concentration of 100µM. The final volume of the test tube should be 5 ml. How much volume of stock solution and growth media should you use to prepare the test tube? Please show your work and use correct units.