Choi, James_Report_Digestion Analysis

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Assignment_12.pdf - Adobe Reader File Edit View Window Help Оpen 1 / 1 99,1% Tools Fill & Sign Comment 3. Recombinant protein is produced by a genetically engineered strain of Escherichia coli during cell growth. The recombinant protein can be considered a product of cell culture even though it is not secreted from the cells; it is synthesized in addition to normal E. coli biomass. Ammonia is used as the nitrogen source for aerobic respiration of glucose. The recombinant protein has an overall formula of CH1.5500.31N..25. The yield of biomass (excluding recombinant protein) from glucose is measured as o.48 g/g; the yield of recombinant protein from glucose is about 20% of that for cells. How much ammonia is required? What is the oxygen demand? If the biomass yield remains at 0.48 g /g, how much different are the ammonia and oxygen requirements for wild-type E. coli that is unable to synthesize recombinant protein? а. b. с. 24°C 08:39 Berawan 27/04/2022

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Activity 2. Media Used in Isolating Coliforms You are a group of microbiologists tasked to test the presence of coliforms in the newly built water station. You are asked to isolate E. coli, Salmonella, and Shigella. Enumerate the media used for each step of isolating these bacteria. Include pictures and references. Bacteria Enrichment (Broth/Agar) Presumptive test (Broth/Agar) Isolation Media (Broth/Agar) E. coli Salmonella Shigella 1. How can you confirm that E. coli, Salmonella, and Shigella are present in the newly built water station? Explain. 2. What is the difference between nutrient broth and nutrient agar? 3. What is the importance of these steps (Enrichment, Isolation, and presumptive test) in isolating bacteria? Conclusion about the process of isolating bacteria

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Below is an image of a western blot and coomassie stain. Need help. I need to know which snack bar (Helfy Bar or RganixSnax) has gluten present. And i need a detailed explanation based on the two tests, as to why the images prove that the product contains gluten.

ANSWER THE FOLLOWING QUESTIONS REGARDING GEL ELECTROPHORESIS 1.why is it not advisable to move/touch the agarose gel in the process of hardening 2.what is the use or function of the TAE buffer that is poured or found in the gel box 3.how can you tell if agel is running 4.outline the process use in the preparation of agarose gel of 1.5 concentration 5.outline the processes/steps used in gel electrophoresis 6.name twoexamples of the dye used in gel electrophoresis 7.how do we prepare x1(concentration) TAE BUFFER form 50x TAE buffer

General Electrophoresis Questions: 1. Why do we usually use agarose for DNA and polyacrylamide for proteins?2. Compare and contrast the sample buffers for DNA and protein electrophoresis.3. Compare and contrast the running buffers for DNA and protein electrophoresis.

Make a flowchart for the procedure of SPREAD PLATE METHOD

Case Study: You are asked to inoculate lactose media (broth) with E. coli that was growing exponentially in glucose media (broth). You monitor the growth of these cells in the lactose broth using a viable plate count technique and plot the Growth Curve for E. coli growing on lactose. After a day of monitoring the cells, you note that the culture entered into lag phase growth initially and is now currently growing exponentially. In addition, you find that the cultures doubling time is 30 minutes. You know that the doubling time of E. coli in glucose media is 20 minutes. You did not change the oxygen content or temperature at which you were growing the cells, E. coli is still aerobically respiring at 37°C. Question: In order to determine the doubling time of E. coli a viable plate countris performed. This means that you are truly counting O Dead cells only via a microscopic count O Live cells only via membrane filtration or serial dilution O All cells via membrane filtration or serial…

Case Study: You are asked to inoculate lactose media (broth) with E. coli that was growing exponentially in glucose media (broth). You monitor the growth of these cells in the lactose broth using a viable plate count technique and plot the Growth Curve for E. coli growing on lactose. After a day of monitoring the cells, you note that the culture entered into lag phase growth initially and is now currently growing exponentially. In addition, you find that the cultures doubling time is 30 minutes. You know that the doubling time of E. coli in glucose media is 20 minutes. You did not change the oxygen content or temperature at which you were growing the cells, E. coli is still aerobically respiring at 37°C. Question: If you want to grow E. coli as quick as possible would you grow it on lactose or glucose? O Mixing the two carbon sources would provide the most optimal growth conditions. O Lactose because of the generation time being faster. O Lactose and an anaerobic environment. O Glucose…

Case Study: You are asked to inoculate lactose media (broth) with E. coli that was growing exponentially in glucose media (broth). You monitor the growth of these cells in the lactose broth using a viable plate count technique and plot the Growth Curve for E. coli growing c lactose. After a day of monitoring the cells, you note that the culture entered into lag phase growth initially and is now currently growing exponentially. In addition, you find that the cultures doubling time is 30 minutes. You know that the doubling time of E. coli in glucose media is 20 minutes. You did not change the oxygen content or temperature at Which you were growing the cells, E. coli is still aerobically respiring at 37°C. Question: If the lac operon is 'on' what does this imply about the cell? O The cell is dying and the lac operon is going to induce apoptosis. Apoptosis is truly cell death and the cells will enter into the death phase of the Growth Curve. O Lactose is being catabolized and glucose is no…

BONUS (15 points) The fallowing series of dilution was prepared from a specimen to determine the number of bacteria. There were 62 colonies on the agar plate prepared by transferring 0.3 ml from the tube number 4. Calculate the dilution factors for each tube. What is the cell concentration in the original specimen? Calculate the total number of cells in tube number 2. étv A) F12 F9 F10 F7 % & %3D delete 5 { T Y J K

mead. Post Lab #2 Smear & Stain Preparation Name: Leidiawa MontaNo Date: 1. Why are thick or dense smears less likely to provide a good smear preparation for microscopic evaluation? Please explain. Becapse, it will diminish the amount ds latcan Pass through by mam 1t difficult to See under the micioscol e, s less liket to Prowde a go image. 2. What could potentially happen if you leave the slide exposed for too long to the open flame? Why do you have to be careful? we can form ring Patterns if we expose the slide for ta0 the flame 3. During the preparation of a smear leading into simple staining of the bacterial culture S. epidermidis you forgot to heat fix the slide. What would you see on this slide as compared to a slide that was properly prepared? Please explain. 4. You partner stained bacterial cells and saw only the background and not the actual cell was stained. Your partner thought this was a mistake. Please explain what type of staining method this is, how it works and why the…

BIOL2201, S24 Dissection 3- Sheep Heart Good source: https://www.youtube.com/watch?v=-ZbXiOrlFJI External Anatomy 1. Set up your dissection in a way you feel comfortable. Use the included video for a suggested method. 2. Make sure to have a camera ready to take pictures of your brain to show you completed the dissection. You will need at least 3 pictures of the eye, more are fine. You will need to identify all of the bolded structures below. 3. Identify the right and left sides of the heart. Look closely and on one side you will see a diagonal line of blood vessels that divide the heart; this line is called the interventricular sulcus. The half that includes all of the apex (pointed end) of the heart is the left side. 4. Locate the coronary arteries and veins that are on the surface of the heart. 5. Find the flaps of dark tissue on the top of the heart. These ear-like flaps are called auricles. 6. The front-most vessel is the pulmonary trunk. Place a probe or pin in the vessel to mark…

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give the respective the TOTAL DILUTION FACTOR of respective serial dilutions. show complete solution.

Font Paragraph Styles 12. What class of Biosafety Cabinet is shown below? C A I Room air Contaminated air HEPA-liltered air Side View

Average plaques for bacteriophage A，B，C are 137，36，25. PFU/ml is average plaques multiply by the volume dilution multiply by the dilution factor. Show your working for each bacteriophage.

explain the steps for restriction and how each procedure of these three routes is performed: 1.SQ 2.IM 3.IV

i. Illustrate the dilution series used and label the final dilution of each dilution.

Which is better for fish treatment Antibiotic or Probiotic? Why? Thank you very much for your help.

A 1.5-year-old child developed vomiting, diarrhea, and fever. Stool sample were inoculated into the Endo media. After 18 hours on the surface of the medium grew medium-sized, round, slightly convex red colonies with a metallic luster. The doctor suspected Escherichia coli. 1. Name the composition of the Endo agar media. 2. Describe the properties of bacterial colonies on the Endo media.  3. What purpose differential diagnostic Endo media used for?

i sincerely need help with these 1. In a table form, list the different Lipase assay methodologies, definitions, advantages and disadvantages 2. Illustrate the reaction sequence of acid phosphatase 3. Illustrate the assay enzyme activity of ALT that uses LDH as indicator enzyme

Give the uses/functions and images of each apparatuses. Basic Laboratory Equipment Uses/Functions Picture   1. Microscope     2. Colony Counter     3. Autoclave     4. Microbiology incubator     5. Drying oven     6. Refrigerator (microbiology)     7. Bunsen burner/alcohol lamp     8. Candle jar     9. Anaerobic jar     10. Microhood or Bacteriologic hood/Safety hood/Safety cabinet

Discuss about the reagents used for agarose gel electrophoresis (not Polyacrylamide gel electrophoresis)

IN YOUR OWN WORDS. Differentiate the following. (Not more than 10 sentences) 1. Aseptic Teachnique vs. Sterile Technique 2. Microbistatic Agent vs. Microbicidal Agent 3. Sterilization vs. Disinfection 4.How is radiation, autoclave and ultrasonic waves used in the hospitals or clinics as a means to inhibit the growthbof microbes? 5. How is filtration applied nowadays in relation to Covid-19?

What is the micro injection molding? (a) Give me product examples are manufactured by micro injection molding from biomedical industry. (b) Can you comment on current situation about microinjection molding in Turkish Industry?