Lab II-DNA Replication1

Slide 10 Please help me explain this on my report on cell dogma

DNA Replication

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Give typing answer with explanation and conclusion to all parts

Slide 14 Please help me explain this picture on my report on cell dogma

Match the enzymes involved in DNA replication with their function. Primase [ Choose ] [ Choose] Synthesizes short RNA segment to initiate new DNA strand Helicase Main enzyme that extends RNA primer by adding DNA nucleotides to it Stabilizes single-stranded DNA Relieves over-winding of DNA ahead of the replication fork Removes RNA primers preceding Okazaki fragment and replaces RNA nucleotides with DNA nucleotides Single-stranded binding proteins Unwinds DNA helix Synthesizes the ends of the linear chromosome Seals nicks between adjacent DNA segments DNA polymerase III [ Choose ] DNA polymerasel [ Choose ] DNA Ligase [ Choose ] Topoisomerase [ Choose ]

Slide 15 Please help me explain this comprehensively

AKS 5b: Which statement is correct regarding the semiconservative nature of DNA? * The semiconservative nature of DNA allows for genetic stability in somatic gene production MRNA operates as a template to allow DNA to replicate itself using ribosomes The structure of the phosphate group on the DNA molecule direct the correct nucleotides into place during replication Nucleotides in each original strand serve as a template for the new strand to be made AKS 5b: Which model accurately represents the semi-conservative nature of DNA replication? * * AA AA AA AA АВ ВА AA BB AA AA АВ АС Figure A Figure B Figure C Figure D

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What is a replication fork?

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The figure below depicts various elements of the eukaryotic replication machinery in action. Enter the name for the protein depicted by each box. Box A Box B Box C Box D Box E Box F DNA polymerase on lagging strand (just finishing an Okazaki fragment) F Maintains polymerase association with DNA Enzyme extends separation of DNA strands Synthesizes RNA fragments that hybridize to DNA Relaxes supercoiled DNA ahead of replication fork Maintains DNA is single stranded state Promotes binding of processivity factors to DNA Newly synthesized strand pocoar Leading-strand template A New Okazaki fragment RNA primer E Lagging-strand template DNA polymerase on leading strand B C D Saaragon - Next Okazaki fragment will start here Parental DNA helix

Slide 7 Please help me explain this on my report on cell dogma

ANALYSIS ANALYSIS: A DNA strand undergoes all the process included in the central dogma. The DNA strand used as a template is given below: Parent strand DNA: DNA daughter strand: 5-AGA-ACT-AAA-CТА-ТСG-СTT-CGT-3' hnRNA: MRNA: original protein: mutated mRNA: mutated protein: second letter A G UUUPhe UCU) UCC UAU UGU Cys UUC U UUA UAC Tyr UGC Ser UAA stop UGA stop| A UAG stop UGG Trp UCA Leu UUG UCG G CUU CCU CAU CGU His CAC САА CUC CC CGC Leu Pro Arg A CUA ССА CG CGA Gln CAG SO AAU CUG CGG AUU ACU AGU AGC Ser A Asn AUC lle A AUA ACC AAC Thr AAA AAG Lys GAU АСА AUG Met ACG AGG Arg GUU GCU GGU U Asp GUC GGC GCC GCA GCG GAC Val GUA Ala Gly A GAG Glu GGG GAA GGA GUG G Translating the unmutated MRNA that was obtained after splicing, the original protein sequence will be? The original protein is [A] (For your answer, use the one-letter symbol (all caps) for the amino acid residues separated by dashes, e.g. C-A-S-H). first letter third letter

DNA Replication: 1. Write in the new (complimentary) strands for each of the two halves of the DNA molecule below to show what the two semi-conserved strands would look like.                  A G T A C C G G G C A A A C T G C A T T G T G                  I  I  I   I  I  I  I  I   I  I  I  I  I   I  I  I  I  I   I  I  I   I  I  I  I  I                     T C A T G G C C C G T T T G A C G T A A C A C                   old strand   A G T A C C G G G C A A A C T G C A T T G T G          new strand:            new strand:           old strand   T C A T G G C C C G T T T G A C G T A A C A C   Gene Expression: 2. Use your DNA strand to construct a part of a messenger RNA molecule.          DNA            A G T A C C G G G C A A A C T G C A T T G T G            mRNA:

Label the figure to assess your knowledge of DNA replication. Drag the appropriate labels to their respective targets Reset] Help Okazaki fragment DNA polymera ase DNA polymerase Nucleotide Leading strand Replication fork Stabilizing proteins Ligase Primase Helicase Lagging strand RNA primer IIIN

answere is wrong i need help identifying the right otder in where they go

DNA strand below: 3’ T A C A T G C C G A A T G C C 5’  Discuss how will replication happen by mentioning the enzyme needed then transcribe to form mRNA. Discuss what will happen to mRNA, then translate, mentioning the anticodon to be used. Look at the genetic code to know what amino acid will become part of the polypeptide chain. 1. Give the discussion of the entire procedure

Picture is only attached as reference. How does the model attached show DNA Replication?What is the importance of DNA Replication?What will happen if there will be an error during the DNA Replication Process?

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Number 1

Y-shaped region created when helicase unwinds a portion of a chromosome that serves as the growing site for DNA replication. origin of replication dispersive replication model semiconservative replication 0 0 0 0 О QUESTION 5 replication fork conservative replication model An enzyme in DNA replication that breaks the hydrogen bonds between the DNA strands to open up or unwind the DNA so it can be copied. DNA polymerase I helicase telomerase 0000 DNA polymerase III topoisomerase QUESTION 6 0 0 0 0 0 One of several processes by which genetic material in the form of "naked" deoxyribonucleic acid (DNA) is transferred between microbial cells. phosphodiester bond antiparallel strands Chargaff's rules complementary base pairs transformation

I. What is the correct order of enzyme action during DNA replication? Number the steps from 1 to 7. HINT: Refer to the slide show and video lecture on this topic to help you solve this one: Synthesis of RNA primers (priming) Ligation II. A double-stranded DNA molecule with the sequence shown below can produce a polypeptide that is four amino acids long. Identify which DNA strands are the coding and the transcribed template strands by circling C or T to the left of the table below, respectively. Use an arrow to indicate the direction of transcription. In the table, show the mRNA sequences and amino acids in this peptide. In spaces to the left and right of the table, label all 5' and 3' ends of all relevant nucleic acid strands. READ CAREFULLY: The table gives you the possibility of filling in answers that show transcription from either strand or in either direction. You are only required to fill in the information relevant to ONE PEPTIDE (no others). Refer to the genetic code on the…

Page 1 of 3 ZOOM DNA Replication_Protein Synthesis_Mutation Assignment *Please type your answers. 1. DNA synthesis and protein synthesis are two processes that are necessary for the cell. Why are these two processes necessary for the cell? How are they connected to each other? wious 4+ 144

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DNA replication Using the labels below, assign them to the appropriate parts of the diagram.

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Activity 4. Study the structure of DNA and answer the questions below. Sugar-Phosphate Backbones Nucleotide Key: O Adenine O Thymine I Guanine I Cytosine Hydrogen Bonds Antiparallel DNA Strands DNA Ladder Double Helix Fig. 9.DNA Structure. Retrieved June 19, 2020 from https://ib.bioninja.com.au/standard-level/topic-2-molecular- biology/26-structure-of-dna-and-rna/dna-structure.html 1. What are the common parts of the nucleotide? 2. Name the different kinds of nitrogenous bases found in the DNA molecule. 3. In DNA what is the paring arrangement of the bases?

דוידודוודי www.www. The above image shows DNA during replication. The new DNA strand build using the top strand as the template would be considered ( Select ] v strand, whereas the new DNA strand build using the bottom strand as the template would be considered ( Select) strand. The lagging strand is synthesized discontinuously and is made up of small fragments called [Select ] v fragments. These fragments contain RNA primers synthesized by [ Select ) ( Select) replaces RNA in the primers with DNA and [ Select ] v glues the fragments together to form a continuous strand. in

All questions  4. In 1957, Matthew Meselson and Franklin Stahl conducted an experiment to determine the model of DNA replication.  a) What is the function of 14N Isotope and 15N Isotope  b) Why do DNA samples need to be centrifuged after the second round of replication? c) What is the main conclusion of their experiment?

to 4 minutes) The schematic diagram below shows organization of the DNA replication fork. Match parts of the diagram (labeled A-F) with the corresponding term from the answer list (designated 31 parental duplex 5' 3' fork progression v A 1Lagging strand 2. An Okazaki tragment 3.Site of action of DNA topoisomerase 4 Leading strand 5. Site of action of DNA helicCase 6.Site of action of DNA ligase

2-5 pls

DNA Replication Drawing Name: Using penci, you will draw a representation of DNA replication along the leading and lagging strands. Follow the directions below, drawing each element in its proper location along the replicating DNA strand. Once you are sure everything is in the correct place, complete your drawing by adding color to distinguish objects as separate. 1. On the diagram below, label the 5 and 3' onds of both parental DNA strands (you can make up which is which) 2 Label the replication fork 3. Draw and label helicase 4. Label the overall direction of DNA replication 5. Draw and label single stranded binding proteins 6. Draw and label the leadng strand 7. Draw and label a single DNA polymerase IIl on the leading strand 8. Draw and label an RNA primer on the leading strand 9. Draw and label a DNA polymerase I on the leading strand 10. On the lagging strand, draw and label at least three Okazaki fragments 11. On the lagging strand. draw and label at least two DNA polymerase IIl…