Expt. 11. Lab Report Filled
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Lab Report
Experiment 11 – Spectrophotometric determination of %Co in purple Co compound
Chemical reaction:
Strong heating
Co(III)(NH
3
)
x
Cl
y
(s)
Co(II)Cl
2
+ NH
3
(g) + N
2
(g) + NH
4
Cl
(purple Co compd.)
Note: In this reaction, the oxidation number of Co in the purple compound was converted from
+3 to +2 after heating.
Data Table:
1. Calibration curve – absorbance vs molarities of Co(II) standards
Table 1: Beer’s law data
C
stock
=
0.09998M (from EDTA titration)
max
= 511 nm (from spectrum)
Solution #
V
s
(mL)
V
t
(mL)
Dilution
factor (DF)
C
f
= C
s
x DF
Absorbance
1
5
25
1/5
0.01998
0.0883
2
10
25
2/5
0.03998
0.1877
3
15
25
3/5
0.05998
0.2867
4
20
25
4/5
0.07998
0.3856
Stock
25
25
5/5
Stock
0.4846
Sample calculation of the concentration of diluted solution:
Solution #1:
1/5*0.09998=0.019996M
Figure 1:
Calibration graph (Beer’s law graph):
Abs. vs. concentration (attach the graph)
Fitting equation:
4.9525x-0.0105
Slope of the line:
4.9525
olar extinction coefficient (
including the units) = 4.9525
2. Determine % Co in the Purple Co Compound
Table 2:
%Co in purple Co compound
# of Trials
1
2
3
Mass of purple Co weighed (g)
0.3905
0.3960
0.3910
Absorbance of Co solution
0.3258
0.3062
0.3156
Molar conc. of Co from
calibration curve
0.06790
0.06394
0.06572
mol of Co = MV (v = 25 mL)
0.001697
0.001598
0.001643
Mass (g) of Co in purple Co =
mole x AM
1.000*10^-1
9.420&10^-2
9.682*10^-2
% Co in purple Co compound
25.61%
24.15%
24.76%
Average % and sd
24.84%
0.733
Sample calculation of % Co:
0.3905*0.025L= 0.001697
0.001697*58.93= 1.000*10^-1
1.000*10^-1/0.3905= 25.61%
(25.61%+24.15%+24.76%)/3= 24.84%
√
(
25.61
−
24.84
)
2
+(
24.15
−
24.84
)
2
+(
24.76
−
24.84
)
2
3
−
1
=
0.733
Answer the following questions:
1.
Why can the spectrophotometric method be used to determine the %Co in purple
compound?
It can measure how much a chemical substance absorbs light by measuring the intensity
of light.
2.
Is this a qualitative or quantitative analysis? This analysis is based on absorption law.
What is the absorption law?
-
Quantitative,
-
A=
bc
3.
The calibration curve is made with Co(II) standard solutions, while the oxidation state of
Co in purple compound is +3. Do you think there is a difference of molar extinction
coefficient between Co(II) and Co(III)? If yes, what preparation should be used before the
determination of Co in this experiment?
-
Yes the
m
olar extinction coefficient will differ.
-
We have to prepare stock solutions with known concentrations in order to calculate
the difference in the molar extinction coeffitient.
4.
What is the
m
olar extinction coefficient (
did you get from your calibration
curve
What is its units?
-
4.9525
-
No units
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