cheer wine lab

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East Carolina University *

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1010

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Chemistry

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Dec 6, 2023

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9

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Lab 7: Ultraviolet-Visible Spectroscopy Yvonne Mwesige The purpose of this lab is to excise the use of calculations for dilute solutions, to perform serial dilutions, identify the basic components and working principles of spectrophotometry, and to generate and use the standard curve to evaluate data. Pre Lab-Questions: 1. Calculate the concentration of a solution made by starting with 25.0 ml of 2.25 M HCl and adding enough water to make the total volume 87.5 ml.
0.64 M 2. A concentration of 355 ppm NaCl in water means that there are 355 mg of NaCl in every liter of water. Determine how many mg of NaCl are present is a 296 ml sample of this solution. 83.3 2. A 100-ppm stock solution of red dye #40 has already been prepared for you by weighing a known amount of red dye, carefully transferring it to a 1-L volumetric flask, and diluting to the mark. How many milligrams of red dye was used when preparing 1 L of the 100-ppm stock solution? Show a calculation to support your answer. Absorbance (A) = C x L x => Concentration (C) = A/(L x ) Ɛ Ɛ 100ppm/(1Lx40) =4,000/1000 = 4ml
Part 1: Preparation of the standard Solutions: Desired Standard Concentration (ppm Stock Solution Concentration (C 1 ) Stock Solution Volume (V 1 ) Dilute Solution Concentration (C 2 Dilute Solution Volume (V 2 ) 2.00 ppm 100 mL 1 2 50 4.00 ppm 100mL 1 4 25 8.00 ppm 100mL 2 8 25 10.00 ppm 100mL 1 10 10 20.00 ppm 100mL 2 20 10 1. Fill in the table below with the information for your standard Solution mL of the 100-ppm stock (C 1 ) (pipette volume, V 1 ) Total mL diluted solution (flask volume, V 2 ) ppm of the diluted solution (C 2 from the calculation) 1 mL 50 2.00 ppm 1mL 25 4.00 ppm 2mL 25 8.00 ppm 1mL 10 10.00 ppm 2mL 10 20.00 ppm
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Red Dye Standards records at wavelength = 501.70 nm SAMPLE Absorbance 2 .104 4 .195 8 .375 10 .473 20 .875 (diluted) Cheer wine .680
Part 3: Determining the Concentration of the Cheer wine ® 1. Using data from the table above, plot the data points for the standards on the graph in your notebook. Be sure your graph has absorbance on the y-axis and concentration on the x-axis. Your graph should occupy the entire piece of paper in landscape format. 2. Draw the best fit line through your data. You can use your calculator, LabQuest, or excel to compute the equation of the best fit line. 3. Write the line equation on the graph in your notebook.
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Post Lab Questions: 1. Why do you think the reference cuvette contained distilled water for the blank? If your unknown used ethanol as the solvent, what would the blank consist of? Ethanol and red dye 2. Justify your choice of wavelength for the analysis of FD&C Red #40. What would happen if you used a different wavelength? The least concentrated dilution to solve wavelength due to more light going through 3. Does the concentration affect absorbance directly or indirectly? How do you know? One factor that influences the absorbance is the concentration because as the concentration goes up, more radiation is absorbed, and the absorbance goes up. Also, beers law 4. What does the slope of the line represent? Absorptivity
5. Do you think you would get the same slope if you recorded your data at a different wavelength? Why or why not? No, if we used a different wavelength, it would change our absorbance concentration and slope. 6. Calculate the absorptivity at 500 nm using the slope of the line. (See the equation on the first page of the handout. Using the equation of the line, determine the concentration of diluted Cheer wine. Show your work in your lab notebook. Concentration of red dye in diluted Cheer wine® ____ 22.1 for 20ppm ________, from the equation of the line. The concentration of red dye in undiluted Cheer wine® ____ 8.25 ________, 7. How many mg of red dye #40 is in each can of soft drink? Show your work in your notebook. 365ml. When you convert it to mg, it equals 360,000mg 8. Briefly describe how spectrophotometry works. Spectrophotometry is a technique to measure light absorption in a solution. It uses a light beam that passes through a sample, and each compound in the solution absorbs or transmits light over a certain wavelength. Conclusion: In this lab, we exercised some calculations for dilute solutions and performed serial dilutions by the principles of spectrophotometry. We used a standard curve to evaluate data like we could plot the graph.
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