Life Chemistry Data Collection (Exp.4)
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Apr 3, 2024
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4 - Life Chemistry Data Collection Student Name: Note: Fill out this sheet for the experiment. It will help you track information for the LAB REPORT
, quiz and exam. Experiment 1, Complex Carbohydrates; Test for Starch Hypothesis: Potato juice will contain starch while glucose, onion juice, albumin, cashew nuts, and vanilla wafers will not contain starch. Independent Variable: Type of samples (water, starch, glucose, potato juice, onion juice, albumin, cashew nuts, and vanilla wafers) Dependent Variable (Data): having starch or not (color change) Two Control Variables (What do you need to make sure stays the same?): 1. Amount of drops of sample 2. Amount of drops of iodine. Positive or Negative Control Experiments (If any, what are they?): Negative control - water Positive control - Starch Table 1. Iodine Test for Starch
Tube #
Content
Final Color
1
Water - Negative Control Orange-red = doesn’t have starch 2
Starch - Positive control
Black = has starch
3
Glucose
Orange-red = no
4
Potato juice
Black = has starch
5
Onion juice
Orange = doesn’t have strach 6
Albumin
Dark orange = doesn’t have starch
7
Chashew Nuts (b)
Dark orange = doesn’t have starch
8
Vanilla wafers (c)
Black = has starch
Rim Seid
Experiment 2, Benedict’s Test for Simple Sugars Hypothesis: Vanilla Wafers will contain simple sugars while starch, potato juice, onion juice, Albumin, and Cashew Nuts will not contain simple sugars. Independent Variable: Type of samples (water, starch, glucose, potato juice, onion juice, albumin, cashew nuts, and vanilla wafers) Dependent Variable (Data): having simple sugars or not (color change) Two Control Variables (What do you need to make sure stays the same?): 1. Amount of drops of sample 2. Amount of drops of Benedict drops Positive or Negative Control Experiments (If any, what are they?): Negative Control - Water Positive Control - Glucose Table 2. Benedict’s Test for Simple Sugars
Tube #
Content
Final Color
1
Water - Negative Control Aqua blue = no simple sugars 2
Starch
Aqua blue = no simple sugars 3
Glucose - Positive Control
Orange = has simple sugars 4
Potato juice
Muddy green = slightly contains simple sugars 5
Onion juice
Orange = has simple sugars 6
Albumin
Brown = no simple sugars 7
Chasew Nuts (b)
Aqua blue = no simple sugars 8
Vanilla Wafer (c)
Orange = has simple sugars
Experiment 3, Biuret Test for Protein Hypothesis: The cashew nuts and the potato juice will contain protein while glucose, onion juice, starch, and vanilla wafers will not contain proteins. Independent Variable: Type of samples (water, starch, glucose, potato juice, onion juice, albumin, cashew nuts, and vanilla wafers) Dependent Variable (Data): contains protein or not (color change) Two Control Variables (What do you need to make sure stays the same?): 1. Amount of drops of sample 2. Amount of drops of Biuret drops Positive or Negative Control Experiments (If any, what are they?): Negative control - Water Positive Control - Albumin protein Table 3. Biuret Test for Protein
Tube #
Content
Final Color
1
Water - Negative control
Light blue = doesn’t contain protein
2
Albumin protein - Positive control
Violet purple = contains protein
3
Glucose
Blue = doesn’t contain protein
4
Potato juice
Grayish-purple = slightly contains protein
5
Onion juice
Green = doesn’t contain protein
6
Starch
Faint-blue = doesn’t contain protein 7
Cashew Nuts (b)
Light violet = contains protein
8
Vanilla Wafers (c)
Blue = doesn’t contain protein
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Experiment 4, Beta Carotene Test for Lipids Hypothesis: The vegetable oil will be soluble in the oil while the glucose, potato juice, onion juice, Albumin, cashew nuts, and vanilla wafers won’t be soluble with the oil. Independent Variable: Type of samples (water, starch, glucose, potato juice, onion juice, albumin, cashew nuts, and vanilla wafers) Dependent Variable (Data): soluble or not Two Control Variables (What do you need to make sure stays the same?): 1. Make sure to shake all the test tubes very well 2. Amount of drops of the oil Positive or Negative Control Experiments (If any, what are they?): Negative Control - Water Positive Control - Vegetable oil Table 4. Beta Carotene Test for Lipids
Tube #
Content
Soluble (Yes or No)
1
Water - Negative Control No
2
Vegetable oil - Positive Control
Yes
3
Glucose
No
4
Potato juice
Yes
5
Onion juice
No
6
Albumin
Yes
7
Chasew nuts (b)
No
8
Vanilla wafer (c)
Yes
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