Caffeine post lab
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Isolation of Caffeine from Tea Isolation of Caffeine from Tea Thomas Khaleel USF CHM2210L Organic Chemistry Lab
Isolation of Caffeine from Tea 2
Introduction
In a standard organic chemistry experiment, caffeine is extracted from tea by sorting chemical components according to how soluble they are in various solvents. In this experiment, the caffeine found in tea leaves is changed into an ionic form that is soluble in water by using the principle of acid-
base reactions. Acidic tannins are converted to their carboxylate salts by using sodium carbonate as a base, which makes them insoluble in the organic solvent dichloromethane. Caffeine from the tea leaves may be extracted selectively using this approach.
Caffeine's solubility properties are essential to the extraction procedure. Due to its high water solubility, caffeine may be easily extracted using hot water from solid materials like the cellulose found in tea leaves. Caffeine is extracted from the aqueous solution using dichloromethane, leaving other substrates in the organic layer behind. The solubility ratio in two immiscible solvents, or the partition coefficient (k),
is a crucial measure for determining how successful the extraction procedure was.
The purpose of this experiment is to provide participants practical experience with the concepts of solubility, acid-base reactions, and extraction methods. After going through this procedure, the extracted caffeine will be further purified and examined. Gaining insight into the real-world uses of organic chemistry in the separation and purification of substances requires an understanding of these ideas. Materials
1.
Tea bags (2)
2.
Scoopula
3.
Deionized water
4.
Sodium carbonate (4g)
5.
125 ml Erlenmeyer flask
6.
Watch glass
7.
Glass stirring rod
8.
Centrifuge tubes (2)
9.
Dichloromethane (DCM) (8 ml)
10.
Caps for centrifuge tubes
Isolation of Caffeine from Tea 3
11.
50 ml Erlenmeyer flask
12.
Sodium sulfate
13.
Pasteur pipette
14.
50 ml beaker
15.
Boiling chip
16.
Heat block
17.
2-propanol (10 ml)
18.
Glass rod
19.
Ice bath
20.
Vacuum filtration apparatus
21.
Nonhazardous solid waste container
22.
Halogenated waste container
Methods
1.
Begin by obtaining two tea bags, carefully opening and recording the mass of the tea leaves. Combine the tea leaves into one bag using a scoopula and staple it securely.
2.
Place the tea bag, along with 30-40 ml of deionized water and 4g of sodium carbonate, in a 125 ml Erlenmeyer flask. Cover the flask with a watch glass and boil the mixture for 20 minutes. Ensure the tea bag remains submerged, using a glass stirring rod if needed.
3.
After boiling, let the flask cool to room temperature. While cooling, gently use the stirring rod to
squeeze out any excess water from the bag.
4.
Remove the tea bag and transfer half of the tea solution to one centrifuge tube and the second half to another. Add 4 ml of dichloromethane (DCM) to each tube, cap, and invert the tubes to achieve homogeneity. Centrifuge the tubes to separate the aqueous and organic layers.
5.
Transfer the bottom layer (organic layer) from each centrifuge tube to a 50 ml Erlenmeyer flask. Repeat the DCM addition, inverting, and centrifuging steps. Combine all extracts into one flask.
6.
Add sodium sulfate to the flask to remove traces of water. Let the mixture stand for 10 minutes, then transfer the clear solution to a clean 50 ml beaker.
7.
Add a boiling chip to the beaker and heat using a heat block, taking care not to overheat since DCM has a low boiling point. Allow the DCM to evaporate, leaving an off-white caffeine residue.
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Acetylcholine
CH₂
acetycholine in original sample:
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H₂O
I
CH3
CH3
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-Å-—•-
CH,—C−O−CH,—CH,—*N–CH,
Acetylcholine
CH₂
acetycholine in original sample:
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I
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Complete the balanced chemical reaction for the following weak base with
a strong acid. In this case, write the resulting acid and base as its own
species in the reaction.
NH:(aq) + HCI(aq) -
14-
O3-
|2+
3+
14+
1
2
3
4
7
9.
1
I5
16
(s)
(g)
(aq)
H3O+
H2O
OH-
CI
Reset
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2.Some CH3CO2H(aq) will ionize, increasing the concentration of CH3CO2–(aq) and increasing the pH.3.Some CH3CO2–(aq) will react with H3O+, increasing the concentration of CH3CO2H(aq) and reestablishing the solution equilibrium.
b. 2 only c. 3 only d. 1 and 3 e. 1, 2, and 3
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Show Transcribed Text
In the laboratory, a general chemistry student measured the pH of a 0.524 M aqueous solution of
pyridine, C,HSN to be 9.461.
Use the information she obtained to determine the K, for this base.
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2
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Consider the following chemical equilibrium: HCOO¯¯ (aq) + H₂O(aq) = HCOOH(aq) + OH¯(aq). Which of the
following graphs represents the perturbation on the system and the change of pH when HCI is added to the container?
|
pH
time
||
III
pH ↑
time
Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer.
C
Q
a
||
=
III
=
d
IV
pH
IV
pH
time
time
K
Open
Χ
Your answer
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In Experiment 4, we used titration to determine the total acid content of samples, which we reported in terms of molarity. Reporting acidity through pH measurements is quite different, in that we can only measure the amount of the acid in its ionized form. You were tasked to investigate a clear aqueous solution of an unknown monoprotic acid. You decided to use two Chem 16.1 methods to gather data.Method 1 – TITRATION: A 10. mL aliquot of the sample was diluted with 25 mL distilled water. Two drops of phenolphthalein were added and then it was titrated 3.54 mL of 0.048 M standardized NaOH to the endpoint.Method 2 – pH STRIP: You took 1 mL of the sample and used a pH strip to estimate the pH, which turned out to be around 3.3. Another 1 mL of the sample was diluted with 9 mL of water. The pH was taken again and is now around 3.8
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