Food Dyes Analysis of Commercial Products

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The data given below was generated by a student in Bio 240L during a Bradford Assay. A standard curve was generated by preparing a set of standard samples of protein (bovine serum albumin) and adding the dye Coomassie Brilliant Blue, When the protein binds to Coomassie Brilliant Blue it absorbed light at 595 nm with an extinction coefficient of 43,000 M-1 cm-1. After the dye binding reaction was complete, absorbance values for each protein standard solution were measured at 595 nm and recorded. Standard # 1 2 13 4 Concentration of protein binded to Coomassie Brilliant Blue (M) 6.35x10-7 1.23x10-6 3.85x10-6 5.68x10-6 OOO Absorbance at 595 nm 0.093 0.176 0.560 0.813 Prepare a standard curve with the student generated data. What is the slope from the standard curve rounded to four sig figs and no units? 1.4 143398 6.972x10-6 1.434x105

An exploration of any one application of Spectroscopy (Absorption or Emission), Mass Spectrometry (MS) - (ANY kind of mass spec)  Pick one location/project/bit of research where the data is used and explore.  Discuss on what is measured (what instrument) and why and how.  You don't have to fully teach the instrument but spend a paragraph giving the very basics of what is measured and how.  And discuss the application you discovered - just find a place the measurement is used and explain what they do - what they measure and when it tells them.

Calculate the molar extinction coefficient (e) of a molecule at a particular wavelength given that a 0.000764 M solution of the molecule produced a fluorescence intensity (I) of 1177 when the irradiance of the beam incident to the sample (Po) was 821 and the length of the medium (b) was 1.12 cm. The proportionality constant k' for this molecule is 0.319. € = ×10 TOOLS M-1.cm-1

Determine the final concentration in mol dm–3 of K+ and SO42– when the following three solutions in volumetric flasks are mixed together: 500 cm3 of 0.20 mol dm–3 KAl(SO4) 2; 1000 cm3 of 0.050 mol dm–3 K2SO4 and 500 cm3 of water. The concentration of K+ is       Select one: a. 0.20 × 103 mol dm–3 b. 0.20 mol dm–3 c. 0.10 × 10–3 mol dm–3 d. 0.10 mol dm–3

When opening the cover of an analytical detector, which optical elements can help us to differentiate whether the instrument is a spectrofluorometer or a UV-Vis spectrophotometer. Justify your answer:

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Given C6H12O2 chemical formula. chemical shift , intergration, multiplicity and interpretation needed.

ica O CHEM-3A-81091-2... ead W ical Part A PCl5 (g) PC13 (1) + Cl2(g) Use A for [PC15], B for [PC13], C for [Cl2]. ΜΕ ΑΣΦ Keq AC1.8 Submit Previous Answers Request Answer × Incorrect; Try Again; 3 attempts remaining Part B 2KNO3(s) = 2KNO2 (s) + O2(g) Use A for [KNO3], B for [KNO2], C for [02]. Keq = ΜΕ ΑΣΦ Submit Part C Request Answer Pollen tomorrow ? ?

It is found that up to 0.0110 g of SrF2 dissolves in 100 mL of aqueous solution at a certain temperature. Determine the value of Ksp for SRF2. 1 2 NEXT > Based on the given values, fill in the ICE table to determine concentrations of all reactants and products. SFF2(s) Sr**(aq) 2 F (aq) + Initial (M) Change (M) Equilibrium (M) 5 RESET 0.0110 8.76 x 10-5 8.76 x 10-4 1.75 x 10-4 1.75 x 103 -0.0110 -8.76 x 10-5 -8.76 x 10-4 -1.75 x 10-4 -1.75 x 103 +x +2x -2х -X 8.76 x 10-5 + x 8.76 x 10-5 1.75 x 10-4 + 2x 1.75 x 10-4 - 2x