Project Proposal:
AIMS
- Bioinformatic approach to aid gene identification and characterisation in Arabidopsis thaliana
- Evaluate and integrate the accuracy of Arabidopsis database
INTRODUCTION:
Arabidopsis thaliana is a model plant for research and has been used wisely to study many aspects of plant biology. There is significant amount of information about this plant in the database, such as fully sequenced and annotated genomic sequence, extensive expressional data and functional characterisation data. This project aims at using such information to aid gene identification.
The project will focus on a specific region (between AGIs 18,500,000 & 19,800,000) on Chromosome V, where a gene involved in root development has
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1999).
Arabidopsis thaliana is the first plant for which the complete genome has been sequenced. The 120 megabase genome of Arabidopsis is formed into five chromosomes and consists of an approximate 20,000 genes(Sato, Kotani et al. 1997) . In this project we will focus on a specific region on chromosome V, where a gene involved in root development has been mapped.Chromosome V is 26 mega bases long and it also the second largest regions of genome(Tabata, Kaneko et al. 2000). The entire genome of chromosome 5, excluding the centromeric and telomeric regions, was covered up by two large contigs 11.6 Mb and 14.2 Mb long separated by the centromeric region(Kotani, Sato et al. 1997).The 5,874 genes encoded on chromosome 5 reveal several new functions in plant, and the patterns of gene organization provides insights into the mechanism and extent of genome evolution in plants(Tabata, Kaneko et al. 2000).
Arabidopsis thaliana is a member of the mustard family Cruciferae or Brassicaceae with a natural distribution all around Europe, Asia, and North. Many different ecotypes have been collected from natural populations and are available for experimental analysis. The Columbia and Landsberg ecotypes are the accepted standards for genetic and molecular studies. The whole life cycle, including seed germination, formation of a rosette plant, bolting of the main stem, flowering, and maturation of the first seeds, is completed over 6 weeks. When it comes to size, almost
Exercise 3A is a study of mitosis. You will simulate the stages of mitosis by using chromosome models.You will use prepared slides of onion root tips to study plant mitosis and to calculate the relative duration of the phases of mitosis in the meristem of root tissue. Prepared slides of the whitefish blastula will be used to study mitosis in animal cells and to compare animal mitosis and plant mitosis.
Asexual Propagation is the process of using plant materials such as the stems, leaves, and roots to multiply the number of plants. These plants eventually grow to be a brand new plant that is genetically identical to the parent plant it came from. In several types of plants, asexual propagation is the fastest means of new plant growth. Asexual propagation is also a good way to maintain a plant species because they are genetically identical. In this process, adventitious roots are seen in the growing cycle. Adventitious roots are those that grow form parts of the plant that they normally would not grow from. The cuttings must do
This report presents an overview to: meiosis, chromosomes, traits, genotypes, and phenotypes displayed in the evolution of fast plants. Studying how the genetic information can be passed along one generation to the following. Also, learning various techniques to determine the possible genotypes of the four Wisconsin Fast Plants provided by analyzing the offspring and observing the phenotypic variation within them. Predicting that the parent plant was heterozygous with the first generation also displaying heterogeneous characteristics of non- purple stem/ Green leaves. And discovering that the null hypothesis was rejected for the chi-square being less than 5% meaning the observed phenotypes were due by chance.
This experiment, which was used to explore the Theory of Evolution created by Charles Darwin. The use of natural selection was apparent in the artificial modification of an organism's traits which aided in this investigation. Through this experiment the Wisconsin Fast Plant was used. It is a fast-growing organism developed to improve the resistance to disease in cruciferous plants. This plant aids scientist in the exploration of environmental effects on population due to the speed to which is matures and reproduces. Artificial selection was stimulated by the selection against plants with few hairs(trichomes). Trichomes create a wider variation which means it is polygenic. The plants that had only a few trichomes were
Round seeds (R) are dominant to wrinkled seeds (r), and yellow seeds (Y) are dominant
At the start of this experiment we were required to obtain a set of four Wisconsin Fast Plants, which are genetically, known as Brassica rapa. These plants have been, “originally selected under continuous fluorescent light to grow and reproduce quickly for research purposes, these petite, fast-growing plants have been used for teaching biology concepts” (Wisconsin Fast Plants). These four pots that contain our plants will be under our watch for the next 16 weeks where we will show our results at the end of the semester.
might affect the fitness of each variant. In other words which factors might increase plant growth, survival,
This experiment began on the first day of lab by planting 12 total seeds from the F1 generation in six individual cells. Potting soil was added until each cell was a little
The basic plant life cycle with alternation of generations is modified in angiosperms by the change of haploid (n) and diploid (2n) generations, which take turns making each other.
The experiments purpose was to understand and observe the gene expressions in the genes pCNT103, cig1 and GapC in the shoot, root and callus tissues of the tobacco plant, Nicotiana tabacum. Using various genetic laboratory research techniques completed the experiment. The experiment consisted of four parts. The sterile tissue culture technique was used to differentiate the tobacco callus tissue in
No one particular organism is an exact replica of another. Diversity in the world is key for future generations to adapt to the fast changing world. This lab observed the corn plant of the Zea mays species to determine the genotype for the gene of tall versus dwarf in unknown parent corn plants by observing the seedlings produced. It was hypothesized that one parent is heterozygous while the other is homozygous recessive. The predicted results were that half of the seedlings would contain the tall gene and the other half would have the dwarf gene. Plants were grown for two weeks under
It has a relatively small genome which has allowed for scientists to fully sequence its genome. Because of the close evolutionary relationship between all flowering plants, deciding to learn about just one plant immensely allows for scientists to get insight about the cell and molecular biology of this whole class of organisms. Arabidopsis thaliana can be grown indoors in large numbers, producing thousands of offspring per plant in a matter of 8-10 weeks. This touches back on what I said earlier about
Making the onion tip root cell slide was successful. Our results supported the hypothesis because we saw cells in the onion root tip in prophase, metaphase, and anaphase. As we went up in power objectives, each phase of the cell became more definitive. The cell root was a great indicator of the structures of the different cycles of the cell. This is important because we will be prepared for future labs working with the microscopes and can now adjust it for the best view of the slide. We practiced working with the compound light microscopes and different phases of the cell cycle. Onion root tips are useful to observe mitosis because the cells are frequently diving as the root grows. So when we stained the cell, we caught many cells in different phases. The significance of this lab was to better understand the process and stages of mitosis and meiosis and compare and contrast the mitotic process in plants and animals. We grasped the concepts of what the chromosomes look like, and what they look like in each step of the processes. Having read much about mitosis and meiosis, seeing these cells was the real application of describing and understanding the stages.
Mitosis is a vital process for all cells where one single cell divides into two identical daughter cells. This procedure encompasses four distinct phases called prophase, metaphase, anaphase, and telophase in order to ensure cell regeneration and growth. A stage called interphase where the cell grows and undergoes DNA replication is a vital part to cell division, which precedes mitosis. The onion root lab aims to view two distinct parts of the specimen’s tip, area x and y, under a light microscope to determine at which location cell division happens at a more frequent rate. If area Y, the middle right section of the onion root, is observed, then there will be a smaller concentration of cells undergoing cell division. If the area X, the bottom left section of the onion root, is observed, then there will be a greater concentration of cells undergoing cell division.
This data shows a strange outcome, in the hypothesis; it says that “If acid is introduced to the seed during germination, then the roots will not grow as long as the seeds that are given water”. This statement proves to be untrue, because the roots grew longer with stronger acid than weaker acid, and in some, cases, grew better with strong acid than it did in water. This may be true because of the acid growth theory. The acid growth theory states that auxins cause the elongation of stem cells by promoting wall loosening. It was determined that this wall loosening is caused by hydrogen ions. This idea and subsequent supporting data gave rise to the acid growth theory, which states that when exposed to auxins, susceptible cells excrete protons into the wall at an enhanced rate, which in turn decreases the pH in the wall. The lowered wall pH then activates the wall loosening process which is essentially doing the same thing as the auxin hormone.