Classifying Glucokinase in Bacteria Essay

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Glucokinase (glk A) is classified in bacteria based upon having ATP binding site and ROK motif, the sequence of glk A gene (JN645812) of S. aureus ATCC12600 showed presence of ATP binding site and ROK motif. We have earlier observed glk A of S. aureus has higher affinity towards the substrate compared to other bacterial glk A and under anaerobic condition with increased glucose concentration S. aureus exhibited higher rate of biofilm formation. To establish this, 3D structure of glk A was built using homology modeling method, the PROCHECK and ProSA-Web analysis indicated this built glk A structure was close to the crystal structure. This structure was superimposed with different bacterial glk A structures and from the RMSD values it is …show more content…

Glycolysis is a major pathway in S. aureus; 85%-90% of the glucose is catabolised through EMP pathway[3, 29]. The very first step of glycolysis is the formation of G-6-P, this is the ubiquitous anabolic intermediate that has essential role in the pathogen from energy generation in the catabolic reactions and upregulation of polysaccharide intracellular adhesion synthesis, virulence factors, cell wall synthesis and formation of SCV are the key characteristic features of drug resistant strains like MDR and VRSA. Polysaccharide intercellular adhesion is a polymer of N-acetyl glucosamine involved in the formation of adhesive exopolysaccharide matrix. This provides structural stability to biofilms, enhanced adhesion to surfaces made protection from host defenses and antibiotics[10, 36]. Majority of G-6-P formation is catalysed by cytoplasmic glk A, this glk A in both Gram positive and Gram negative bacteria comprises of 315-321 residues with a monomeric mass of 33-35 kDa, Km values of glucokinase varied from 0.3-0.8mM for glucose[29]. Glucokinases of bacteria are divided into 2 groups (1) Glucokinases that belongs to ‘Repressor/open reading frames of unknown function/sugar kinases’ (ROK) family which is characterized by the presence of CXCGX(2)GCXE motifs, and (2) Glucokinases without ROK motifs. However, in Archaea two types of ATP-dependent glucokinase are

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