Compound X As An Inhibitor Of Bromelain

Data Tables and Observation: Fill out the data sheet (below) for the experiment and submit with this form. Recording data carefully

And finally into test tube 3, I pipetted 1.0 ml turnip extract and 4.0 ml of water. The contents of test tube 1 was poured into a spectrometer tube and labeled it “B” for blank. “B” tube was now inserted it into the spectrometer. An adjustment to the control knob was made to zero the absorbance reading on the spectrometer since one cannot continue the experiment if the spectrometer is not zeroed. A combination of two people and a stop watch was now needed to not only record the time of the reaction, but to mix the reagents in a precise and accurate manner. As my partner recorded the time, I quickly poured tube 3 into tube 2. I then poured tube 2 into the experiment spectrometer tube labeled “E” and inserted it into the spectrometer. A partner then recorded the absorbance reading for every 20 seconds for a total of 120 seconds. After the experiment, a brown color in the tube should be observed to indicate the reaction was carried out. Using sterile techniques, any excess liquid left was disposed

In order to control all of these factors throughout the experiment, one must randomly select the alleles (cheerios), must have a larger population size

designing an experiment, we have to design an experiment and we need to confirm the

I put one test tube for each control, substrate, and enzyme in the 4° C (ice bath), 23° C (room temperature), 37° C (body temperature), and 60° C (water bath). Add the inhibitor that was used and what it was used for.

Research Question: This investigation will determine the how the amount of water (independent variable) affects the amount the amount of time (dependent variable) it takes for the Alka-Seltzer to dissolve.

There are several parts to the experiment such as both the independent and dependent variables. The independent variable is controlled or changed during the experiment to test the effects on the dependent variable. The dependent variable is tested and measured during the test .A controlled variable is an example for an independent variable would be variable that is held constant throughout the experiment. An example is a theory that could extend a person’s life expectancy. The independent variable is the amount of vitamin given to the subject within the experiment. The dependent variable is the life span

1. For this experiment, what were the independent variable and the dependent variable? What were the constants?

b. Dependent Variable: the variable that I am measuring (it depends on the independent variable)

Next the beetle was weighed and it’s weight was recorded. The dental floss was then tied around the second and third legs, and taped to the petri dish. A strip of cloth was also needed to provide traction for the beetle. Then, the petri dish was removed from the beetle and the weight of the petri dish was recorded. The experiment was then ready to be performed. Paperclips were added to the dish every 5-10 seconds until the beetle could pull it no more. Then, the petri dish was removed from the beetle and the weight of was recorded. This experiment’s dependent variable was the amount of mass the beetle can pull, and the independent variable was the mass of the beetle. There was no control group in this experiment. These were the materials and methods used to conduct this

There were three test tubes in which the experiment was held. A relatively equal sized portion of raw potato (this contained the enzyme [a biological catalyst] hydrogen peroxidase) was placed in each tube. Then, enough water to cover the potato was added. Proceeding this, each of the test tubes were assigned a temperature; cold, room temperature or warm (this was written on the tag so that they were not confused). The test tube destinated ‘cold’ was placed in a ice bath for five minutes. At the same time, the ‘hot’ test tube was placed in a hot water bath for five minutes. Meanwhile, the room temperature test tube sat at room temperature for five minutes. When the five minutes were over, the test tubes were returned to the rack (so that they were able to be observed). Then, the test tubes were allowed to sit at room temperature for five more minutes. Once that period of time was over, 2 ml of hydrogen peroxide (the substrate) was added to each tube.

Every 30 seconds their preferred position was documented. Once the three minutes were up and all the information was documented then the ten pill bugs were gently collected and put back in to their jars. These pill bugs were then traded with another tables pill bugs. The new pill bugs came from a sucrose and water chamber habitat. Again ten of these pill bugs were gently grabbed and placed in the chamber for 3 minutes with every 30 seconds documenting where the pill bugs preferred. After the 3 minutes the pill bugs were grabbed placed back in to their jars and switched again with a different table. The last trial of pill bugs came from a tea and water habitat. The ten pill bugs were gently grabbed and placed in to the chamber for three minutes. Every 30 seconds their preferred habitat was documented. When the 3 minutes were up the pill bugs were gently grabbed and placed back in to their jar. In this experiment the control was the distilled water, the dependent variable was the number of bugs in the habitat preferred, and the independent variable was the alcohol, and the water

To ensure the experiment was kept as a fair test a number of variables were controlled. The temperature of the solutions was kept constant by placing the boiling tubes into a test tube rack and setting it into a water bath with a fixed temperature of 25oC. The temperature needed to be kept low and fixed as a high temperature would denature the enzymes, they would therefore be unable to break down the gelatine and no results would be produced from the experiment. Keeping a constant temperature also meant that the solutions reacted at the same rate.

The purposes of this experiment were to model a bimolecular nucleophilic substitution reaction between potassium hydroxide (KOH) with 1-bromopropane and determine whether it follows a second-order rate law mechanism. A rate constant of 0.0684 M-1 min-1 was obtained for this reaction at 45.1°C, which was determined through equilibrating the reaction and performing titrations of 0.390 M KOH with 0.1000 M hydrochloric acid (HCl). The activation energy calculated from class data was 50.188 kJ/mol, which deviated largely from the literature range value of 72.80–83.76 kJ/mol. It was concluded that the reaction was consistent with the predicted SN2 mechanism, based on the regression of a trendline.

Then, each group of students received the necessary materials to complete the experiment. When the students received the cups, they labeled cups to distinguish between the salt solution, distilled water, and control group. After weighing the cups and finding the mass of the cucumbers, the students poured 50 ml of water in one cup, 50 ml of salt solution in the other, and left the control cup empty. Then, the students placed the cucumbers into the cups and waited 30 minutes for the results. After the 30 minutes, the students removed the cucumbers from each solution and dried the cucumbers with paper towels. The students then weighed the cucumbers again and recorded their results. Lastly, the students found the difference from the original mass of the cucumbers and recorded their results.