Enzyme Activity Lab Report

Use ice if you need to. Then, fill one beaker with 175 mL of water and the other with 350 mL. Warm the water in the 350mL beaker up to 55 degrees celsius and cool the water in the 175mL beaker to 15 degrees celsius, the same temperature as the pitcher because it will be your control group. Once the beaker that should be heated is at 55 degrees celsius, pour 175 mL of the water into a glove and pour the other 175 mL into a ziplock baggie. Pour the 15 degrees celsius, 175 mL of water into another ziplock baggie. Before you set these in water, have a stopwatch ready and make sure that the water in the baggies and glove is at the right temperature.

Step 4: Place the test tubes into separate coffee cups to maintain the upward position. Add 2 mls of the catalase solution to each of the test tubes and then place tubes1, 2, and 3 in the conditions described above. For test tube 4, fill the coffee mug half full of boiling

The hypothesis tested in this experiment was, if the temperature of enzyme catalysis were increased, then the reaction rate would increase, because enzyme-catalysis reacts by randomly colliding with substrate molecules, and the increase in temperature increases the speed of collision or reaction rate. The final data collected for the experiment was positive with my hypothesis. The coffee filter, covered in potato solution, sank and rose at a faster pace in the hydrogen peroxide when the temperatures were raised.

Fill a test tube about 1/3 full with cold tap water for use in step 34.

Create a water bath by filling ½ of the 100 mL beaker with cool water, adding crushed ice to the beaker so the water level is just below the top, and sprinkling salt into the beaker

40 g of ice and approximately 30 ml of sulfuric acid is cautiously added to a 100 mL beaker respectively. Weigh 7.6 g of ammonium chloride and 14.0 g of ammonium bromide and place it in another beaker, crushing the lumps until a powdery mixture remains. The powdery mixture is then transferred to a 125 mL Erlenmeyer flask. Add the ammonium salts into the sulfuric acid mixture. Heat is applied to dissolve the salt. Once the

b. Place crushed ice in the beaker so the water level is just below the top of the

2) Rinse the solid with about 30 mL of distilled water and decant the liquid from the solid. It is critical that as little solid as possible is lost during this process. Repeat the rinsing two or three times.

17. Put the catalase solution with a cap into the ice chest. 18. Pour 40 mL of hydrogen peroxide into one of the canning jars. Pour 40 mL of hydrogen peroxide into the other 3 jars and put two of them in the refrigerator and one in the freezer with lids on.

Procedure: Cut top off 2 liter bottle and bottom off another. Fill the one without a top with water and put a

precipitate; then pour over the solid in the funnel. Finally, pour two 10-mL portions of

How different temperatures will affect the catalase enzyme Mrs Shiell K1 Kgothatso Kekana gr10 Introduction Enzymes are biological molecules (typically proteins) that significantly speed up the rate of virtually all of the chemical reactions that take place within cells. Catalase is an enzyme which is found in almost all living organisms. This enzyme helps to convert hydrogen peroxide into oxygen and water.

8. Use the plastic forceps to remove the potato cylinders from the beakers (keep them together in the same group), and blot off the excess solution weight on them with the paper towels.

Measure the correct amounts of sugar and water into each of the labeled cups. One at a time, place a cup on the scale and add the sugar directly, but before you do so, hit the button “on” or “zero” to restart the scale. This way, the scale will only measure the amount of sugar that is poured into the cup and not the cup itself. The chart shown below will tell you how much sugar to weigh in each cup. Once you have finished, add 50 mL of water to each cup using the graduated cylinder. Then stir your cups with the spoon until the sugar has dissolved in each solution.

The flavoured, concentrate is transferred to a 12,000 litre storage tank, containing a chilled syrup solution. The tank is chilled to around 2°C.