Hepatocellular Carcinoma Essay

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Introduction: Hepatocellular carcinoma (HCC) is the fifth most common malignancy worldwide and the second cause of cancer-related mortality in eastern and western countries [1]. Each year, >700,000 patients diagnosed as HCC and morbidity rates of HCC continue to increase, possibly due to deterioration of environment, unhealthy dietary habits and other related factors [2]. Reversion-inducing-cysteine-rich protein with kazal motifs, also known as RECK, is a human gene, thought to be a metastasis suppressor against activated ras oncogenes [3]. It is a promising goal for controlling tumor invasion and angiogenesis. Although RECK mRNA is expressed in most of normal human tissues and untransformed cells, the protein level is low or…show more content…
Sampling: Under complete aseptic conditions, 8 ml of venous blood were collected .Each blood sample was divided and divided as follows: Tube A :4 ml were collected in plain tube, left to clot at 37ºC ,then centrifuged ,clear sera were separated and divided into 3 aliquots; the first one was used to determine liver function tests(total &direct bilirubin, AST, ALT, GGT,ALP, total protein and albumin) by using AU640 autoanalyzer. The second was used for measurement of alpha feto- protein by enzyme-linked immunosorbent assay using Human AFP ELISA kits supplied by Glory Science(Immunospec Corporation, 7018 Owensmouth Ave. Suite 103 Canoga Park, CA, 91303) and the third one used for detection of hepatitis markers antibodies (HBsAg, and HCV antibody) by direct sandwich assay using the ELISA Kit supplied by Adltis, Germany. The specimens were kept frozen at -20 ˚C until the time of assay, Tube B: 2 ml were collected in a sterile sodium citrate vacutainer tubes for immediate assay of prothrombin time, concentration and INR and Tube C: 2ml were collected in sterile vacutainer tubes containing EDTA for DNA extraction. Evaluation of RECK gene polymorphisms DNA analysis: Polymerase chain reaction-restriction fragment length polymorphism PCR-RFLP method was used to
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