. If you forgot to add dNTPs to a sequencing reaction, what would be the result? Only very long fragments would be synthesized Only very short fragments would be synthesized The fragments would not be labelled DNA polymerase would be inactivated Sequencing would proceed normally

Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
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Chapter15: Genomes And Genomics
Section: Chapter Questions
Problem 6QP: Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel,...
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a. If you forgot to add dNTPs to a sequencing reaction, what would be the result? Only very long fragments would be synthesized Only very short fragments would be synthesized The fragments would not be labelled DNA polymerase would be inactivated Sequencing would proceed normally b. Please also answer the image
You are performing a PCR as diagrammed below (green arrows indicate primer binding sites). However, your DNA sequence contains an insertion (additional sequence) shown in red. What effect will this have on the PCR product produced?
5'
3'
3'
5'
The PCR product will be larger than without the insertion.
The PCR product will be smaller than without the insertion.
The PCR product will be the same size regardless of the presence of the insertion.
The PCR reaction won't work in the presence of the insertion.
Transcribed Image Text:You are performing a PCR as diagrammed below (green arrows indicate primer binding sites). However, your DNA sequence contains an insertion (additional sequence) shown in red. What effect will this have on the PCR product produced? 5' 3' 3' 5' The PCR product will be larger than without the insertion. The PCR product will be smaller than without the insertion. The PCR product will be the same size regardless of the presence of the insertion. The PCR reaction won't work in the presence of the insertion.
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