1. How does the resin facilitate purification in glutathione S-tranferase (GST) affinity? 2. Does protein stick to the resin in glutathione S-tranferase (GST) affinity? If so how do you get it off? 3. Explain how protein of interest would be purified in glutathione S-tranferase (GST) affinity?

1. How does the resin facilitate purification in glutathione S-tranferase (GST) affinity? 2. Does protein stick to the resin in glutathione S-tranferase (GST) affinity? If so how do you get it off? 3. Explain how protein of interest would be purified in glutathione S-tranferase (GST) affinity?

Biochemistry

9th Edition

ISBN:9781305961135

Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Publisher:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Chapter3: Amino Acids And Peptides

Section: Chapter Questions

Problem 13RE: MATHEMATICAL An organic chemist is generally happy with 95% yields. If you synthesized a polypeptide...

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Chemistry

1. How does the resin facilitate purification in glutathione S-tranferase (GST) affinity?

2. Does protein stick to the resin in glutathione S-tranferase (GST) affinity? If so how do you get it off?

3. Explain how protein of interest would be purified in glutathione S-tranferase (GST) affinity?

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