1. The circular plasmid PRIT453 was cut with Smal, HindlII, and EcoRI, singly and in pairs. The fragments resulting from these enzyme digests are shown below. (all sizes given in kb) EcoRI HindIII Smal EcoRI + HindIII 7.7 7.4 6.6 4.5 5.7 2.7 1.6 1.9 2.7 1.9 2.0 EcoRI + Smal Smal + HindlIII 1.6 1.3 0.9 0.7 2.2 1.9 2.5 A) How many times is each enzyme site present in the plasmid? How big is the plasmid (in kb)? Determine the map. You can make it circular or linear. C)

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1.
The circular plasmid pRIT453 was cut with Smal, HindlII, and EcoRI, singly and in
pairs. The fragments resulting from these enzyme digests are shown below. (all sizes given in
kb)
EcoRI
HindIII
Smal
EcoRI + HindIII
7.7
7.4
1.6
1.9
6.6
4.5
5.7
2.7
2.7
1.9
2.0
1.6 1.3
0.9 0.7
2.2 1.9
EcoRI + Smal
Smal + HindlIII
2.5
A)
How many times is each enzyme site present in the plasmid?
How big is the plasmid (in kb)?
Determine the map. You can make it circular or lincar.
Transcribed Image Text:1. The circular plasmid pRIT453 was cut with Smal, HindlII, and EcoRI, singly and in pairs. The fragments resulting from these enzyme digests are shown below. (all sizes given in kb) EcoRI HindIII Smal EcoRI + HindIII 7.7 7.4 1.6 1.9 6.6 4.5 5.7 2.7 2.7 1.9 2.0 1.6 1.3 0.9 0.7 2.2 1.9 EcoRI + Smal Smal + HindlIII 2.5 A) How many times is each enzyme site present in the plasmid? How big is the plasmid (in kb)? Determine the map. You can make it circular or lincar.
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