2. A plasmid was digested with the enzymes BamHI, ECORI, and HindIII. A single digest was performed with each enzyme and then double digests were performed. Make a restriction map of the plasmid based on the data below and indicate the distances between the restriction sites Plasmid cut BamHI EcoRIHindIII with: DNA 87.2 fragments (bp) EcoRI + EcoRI + ВатHI + HindIII HindIII ВатHI 87.2 87.2 79.6 7.6 72.2 64.6 15 22.6

Biology: The Dynamic Science (MindTap Course List)
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ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Chapter18: Dna Technologies: Making And Using Genetically Altered Organisms, And Other Applications
Section: Chapter Questions
Problem 3TYK: Why are antibiotic resistance markers such as ampR important components of bacterial plasmid cloning...
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2. A plasmid was digested with the enzymes BamHI, ECORI, and
HindIII. A single digest was performed with each enzyme and
then double digests were performed. Make a restriction map of
the plasmid based on the data below and indicate the distances
between the restriction sites
Plasmid cut BamHI EcoRIHindIII
EcoRI +
EcoRI +
ВатHI +
with:
DNA 87.2
fragments
(bp)
HindIII
ВатHI
HindIII
87.2
87.2
79.6
7.6
72.2
64.6
15
22.6
Transcribed Image Text:2. A plasmid was digested with the enzymes BamHI, ECORI, and HindIII. A single digest was performed with each enzyme and then double digests were performed. Make a restriction map of the plasmid based on the data below and indicate the distances between the restriction sites Plasmid cut BamHI EcoRIHindIII EcoRI + EcoRI + ВатHI + with: DNA 87.2 fragments (bp) HindIII ВатHI HindIII 87.2 87.2 79.6 7.6 72.2 64.6 15 22.6
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