1. What is the purpose of preparing a cell culture? 2. Differentiate the general types of cell culture. 3. Enumerate and describe the role of the different components of a cell culture media.
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1. What is the purpose of preparing a cell culture?
2. Differentiate the general types of cell culture.
3. Enumerate and describe the role of the different components of a cell culture media.
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Solved in 4 steps
- 1a.) How pH of the culture medium affects the cell culture? Please give examples of low pH and high-pH culture conditions. 1b.) Describe the difference between defined and undefined cell culture media. What are the advantages and disadvantages of both of them?1. What is the actual magnification of Cell X? Explain the solution .Which cells are or have been made totipotent? Select all that apply A) a zygote B) sperm cells C) plant cells in the right experimental medium D) human skins cells in the right experimental medium
- 1.Describe the difference between the appearance of surface and subsurface colonies in a pour plate. If this is the same bacterial species, why do these differences in colonial growth occur? 2.Define the term "colony" as it relates to bacterial growth on solid media.1. Calculate the CFU/mL of the original culture for the countable plate as shown in the diagram. b) How many colonies would you expect if you plated out 0.1 mL from Tube C. c) How many colonies would you expect if you plated out 1.0 mL from Tube C.Features of continuous cell culture include the following, except ________ . a.addition of product b. stirring c.addition of nutrients d.removal of product e.sterilization of nutrients
- describe the macro and microenvironments within a cell culture laboratory.1. What are the advantages and disadvantages of viable cell counts and turbidimetric method? Discuss it comprehensively and please, do not jus copy from somewhere.Explain the statements below: 1.Animal cell cultures requires high technical skills to interpret and to regulate animal cell culturing. 2. Animal cell culture makes use of low amounts of reagents.
- 1. The physical factor influencing water entry into and exit from cell interior resulting in microbial cell distention or shrinkage is a.Osmotic pressure b.Hydrostatic pressure c.Radiation d.Temperature 2. The following is/are true about plate count EXCEPT a.A plate count is a count of viable or live cells b.Is based on the principle that viable cells replicate and give rise to visible colonies on plates incubated under optimum conditions c.Is based on the assumption that only one cell always lands on a particular spot in the plate and gives rise to one colony d.None of the above PLEASE ANSWER BOTH QUESTIONS, ASAP Please no need for long explanation. WIll rate you fast1. Compare the growth of the culture found in Plates A, B, C and D. Which among the four plates has shown the most growth? Why do you think so? 2. Describe the abundance of the colonies found in the plates. What possible type of microorganisms are found in the plates?a) What is morphology? b) Describe the common morphological characteristics of bacteria that are often used in microbiological studies. c) Explain the chemical and physical requirements needed for bacterial growth d) Explain the importance of using growth medium and devise a STEP-BY-STEP plan to grow bacterial cell cultures in the laboratory.
