Show the nucleotide sequence changes that might arise in a dsDNA (coding strand segment GCTA) upon mutagenesis with (a) HNO2 (b) bromouracil and (c) 2-aminopourine
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- Explain how Benzer’s experimental results revealed thatthe rII region in bacteriophage T4 contains two genes,each composed of many nucleotide pairs.Approximately how many Okazaki fragments are synthesized in thereplication of the human genome?Bacteriophage T4 has a linear double-stranded DNA genome, yet mapping many mutations, as shown in Figure, generates a circular linkage map. How might you explain this discrepancy?
- "The molecule serving as the genetic material is expected to absorb at the wavelengths shown to be mutagenic." Explain this statement ?The following represent deoxyribonucleotidesequences in the template strand of DNA:Sequence 1: 5'@CTTTTTTGCCAT@3'Sequence 2: 5'@ACATCAATAACT@3'Sequence 3: 5'@TACAAGGGTTCT@3'(a) For each strand, determine the mRNA sequencethat would be derived from transcription.(b) determine the amino acidsequence that is encoded by these mRNAs.(c) For Sequence 1, what is the sequence of the codingDNA strand?Given the partial transposons DNA sequence 5’-ACCGTATTCGGT-3’ upstream from the central region, assuming both terminal inverted repeats and flanking direct repeats have 6 base pairs, hypothetically write the transposon structure downstream from the central region.
- Nearly _______of the Human GenomeConsists of Transposable Elements?Examination of the structure of DNA polymerases bound to nucleotide analogs reveals that conserved residues come within van der Waals contact of C-2' of the bound nucleotide. What is the potential significance of this interaction?The DNA of a deletion mutant of λ bacteriophage has a length of 15.4383 μm instead of 19.6356 μm. How many base pairs are missing from this mutant? *
- What proportion of exons are repeated sequences in the human genome? Is 38% surprising?Describe how the association of DNMT1 with the UHRF1 and PCNA proteins ensures that it will be found on hemimethylated DNA.Of the four DNA bases, why is guanine most likely to be modified after exposure to polycyclic hydrocarbons such as benzo(a)pyrene, as shown in Figure 23.8? (Hint: Consider the pKa values of the four DNA bases.)
