13. As you recall, a process called catabolite repression prevents high level of transcription of lacZYAgenes when glucose is present in the growth medium. Low levels of glucose lead to production ofCAMP, which enables CRP to bind to the lacZYA promoter. If the gene encoding CRP were toexperience a loss-of-function mutation, what would be the expected outcome?a. Reduced level of lacZYA transcription in a medium containing only lactose (no glucose)b. No change in lacZYA transcription in a medium containing only lactose (no glucose)c. Increased level of lacZYA transcription in a medium containing only lactose (no glucose)e. Increased level of lacZYA transcription in a medium containing both lactose and glucose

The ability of bacteria to produce some protein only when the substance that it metabolizes is…

Answered: 13. As you recall, a process called…

Biology 2e

2nd Edition

ISBN:9781947172517

Author:Matthew Douglas, Jung Choi, Mary Ann Clark

Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark

Chapter16: Gene Expression

Section: Chapter Questions

Problem 14RQ: A scientist compares the promoter regions of two genes. Gene A’s core promoter plus proximal...

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8 There is a promoter of a tumour suppressor gene in a mutant cancer cell line, and discover that instead of a nucleosome free region 5’ of the transcription start site there are nucleosomes bound. What would be the likely consequence on transcription of this gene? I am not sure about what is the outcome, normally it binds to the nucleosome free region 5' of the transcription start site to initiate transcription. Now, it binds with nucleosomes. What will happened? Is the DNA cannot transcripted successfully? Due to the transcription needs a naked DNA to process while in nucleosomes, DNA has been wrapped up. Thank you.
1. Transcription: a)State the role of RNA polymerase in gene transcription.b. Explain why the DNA is not used directly for protein translation (i.e., why is mRNA used instead?).c. Explain what occurs when a gene’s promoter region is open for RNA polymerase binding.d. Explain what occurs when a gene’s promoter regions is blocked from binding RNA polymerase.e. Explain how two cells, such as liver cells and skin cells, can become specialized in structure and function despite containing the same genome.
1. In a couple of sentences, explain how eukaryotic activator proteins (that are usually gene-specific transcription factors) can enhance transcription even when bound to sequences hundreds or thousands of nucleotide pairs away from a gene’s promoter. 2. Describe three common characteristics of transcription factors. The characteristics may relate to structure, or mechanism of action (PLEASE ANSWER BOTH PLEASE)
1)A. how do you read a sequence of DNA (template or non-template strand) to convert it an mRNA sequence and to a protein? B.How does chromatin remodeling regulate gene transcription? C. What are the major differences between gene expression in bacteria and eukaryotes D. How are non-coding regions involved in gene transcription? E. Explain how eukaryotic genes sometimes produce multiple protein products?
5a The two factors that bind directly to the DNA at Pacific sequences are TFIID and TFIIB- in general terms what is the function of each of these two transcription factors in the initiation of transcription This is not a graded question and it will never be graded, This is for studying purpose only
17.Given that ATG indicates the start of a given gene, the mutation that causes 5'- ATGCTTCCCGGA (underline indicates the reading frame of the gene) to become 5'. ATGCGTTCCCGGA is called a) silent mutation ( b) base-pair substitution () c) frame-shift mutation () d) combined mutation e) beneficial mutation 18. Identify the single most correct choice: 0 a) An inducer can directly bind to a region of DNA and stimulate transcription () b) An inducer can bind to a suppressor and stimulate transcription c) An inducer can bind to an S/D (Shine-Dalgarno) binding site and stimulate transcription d) All of the above e) a and b
Discuss Concepts In a mutant strain of E. coli, the CAP protein is unable to combine with its target region of the lac operon. How would you expect the mutation to affect transcription when cells of this strain are subjected to the following conditions? Lactose and glucose are both available. Lactose is available but glucose is not. Both lactose and glucose are unavailable.
A scientist compares the promoter regions of two genes. Gene A’s core promoter plus proximal promoter elements encompasses 70bp. Gene B’s core promoter plus proximal promoter elements encompasses 250bp. Which of the scientist’s hypotheses is most likely to be correct? More transcripts will be made from Gene B Transcription of Gene A involves fewer transcription factors Enhancers control Gene B’s transcription Transcription of Gene A is more controlled than transcription of Gene B.
The a/a operon is an inducible operon that controls the production of the sugar arabinose. When arabinose is present in a bacterium it binds to the protein AraC, and the complex binds to the initiator site to promote transcription. In this scenario, AraC is a(n). activator inducer repressor operator
For the E. coli lac operon, when lactose is present: a. and glucose is absent, cAMP binds and activates catabolicactivator protein (CAP). b. and glucose is absent, the level of cAMP decreases. c. activated CAP binds the repressor protein to remove it from the operator gene. d. the cell prefers lactose over glucose. e. RNA polymerase cannot bind to the promoter.
A binding .site for RNA polymerase is called a ___ . a. gene b. promoter c. codon d. protein
1. Eukaryotic transcription initiation begins at the TATA site approximately 30 bp upstream of the transcription start site. One subunit of TFIID (Transcription Factor II D) binds at the TATA site. What is this subunit? 2. This initial binding at the TATA site is specific and coincides with the binding of the entire TFIID complex. This results in a massive change of shape to the DNA helix of the promoter region. After TFIID binding and this shape change, another GTF(General Transcription Factor) can bind. What is this next GTF? 3. Next, which GTF binds to a DNA sequence in the promoter region and to the TFIID-promoter complex before the binding of the RNA Pol II-TFIIF complex? 4. Describe how would you redraw Figure 13-16 to reflect the change in the dsDNA helical shape due to the initial binding to the TATA site? 5. What other…

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