13-14. A set of Hfr strains transfer markers in the following order.StrainTransfer orderJIAF G1ED BGFAIJH CE DCED B13. Which of the following gene orders is correct?A: AFGEDBCHIJB: ECHIJAFGBDC: E D B G FACH JID: E D B G FACHIJE: A F G BD ECHJI14. Which Hfr strains have F factors inserted in the same orientation?A: 1 and 3 oriented the same way, 2 and 4 both oriented the other wayB: 1 and 2 oriented the same way, 3 and 4 both oriented the other wayC: 1 and 4 oriented the same way, 2 and 3 both oriented the other wayD: 1 oriented one way, 2, 3 and 4 all oriented the other wayE: all are oriented the same way2UYSerlero

Since we only answer one question at a time according to our honor code, therefore we’ll answer…

Answered: 13-14. A set of Hfr strains transfer…

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter17: Bacterial And Viral Genetics

Section: Chapter Questions

Problem 1ITD

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E. coli cells are simultaneously infected with two strains of phage λ. One strain has a mutant host range, is temperature sensitive, and produces clear plaques (genotype h st c); another strain carries the wildtype alleles (genotype h+ st+ c+). Progeny phages are collected from the lysed cells and are plated on bacteria. The following numbers of different progeny phages are obtained: Progeny phage genotype Number of plaques h+ c+ st+ 321 h c st 338 h+ c st 26 h c+ st+ 30 h+ c st+ 106 h c+ st 110 h+ c+ st 5 h c st+ 6 a. Determine the order of the three genes on the phage chromosome. b. Determine the map distances between the genes. c. Determine the coefficient of coincidence and the interference
Shown below are the complementation test results involving 4 independently isolated lethal mutants in a bacteriophage. Complementation was assayed by simultaneouly infecting bacteria with two phage strains, each with a different mutation, neither of which could alone lyse the cells. In the table below, a "+" indicates the strains complemented each other and therefore lysed open the bacteria. A "0" indicates no complementation and therefore no cell lysis occurred. Test pair Results 1___2___3___4 1,2 + 1 0 + + 0 1,3 + 2 0 + + 1,4 0 3 0 + 2,3 + 4 0 2,4 + 3,4 + Which mutants are in the same gene? . a. 2, & 3 b.1, 2, 3 & 4 c.1 & 4 d.1, 2 & 4
Shown below are the complementation test results involving 4 independently isolated lethal mutants in a bacteriophage. Complementation was assayed by simultaneouly infecting bacteria with two phage strains, each with a different mutation, neither of which could alone lyse the cells. In the table below, a "+" indicates the strains complemented each other and therefore lysed open the bacteria. A "0" indicates no complementation and therefore no cell lysis occurred. Test pair Results 1___2___3___4 1,2 + 1 0 + + 0 1,3 + 2 0 + + 1,4 0 3 0 + 2,3 + 4 0 2,4 + 3,4 + How many genes are there? a. 3 b.1 c. 2 d. 4
Consider the five E. coli merodiploid strains listed here. Strain #1 I+P+O+Z-Y+/I+P+OcZ+Y+ Strain #2 I+P+O+Z+Y+/I-P+OcZ+Y- Strain #3 I+P+O+Z-Y+/I-P+OcZ+Y- Strain #4 I-P-O+Z+Y-/I+P+OcZ-Y+ Strain #5: ISP+O+Z+Y+/I-P+O+Z+Y- Which of these strains will express b-galactosidase constitutively? Select all correct answers. A.)Strain #5 B.)Strain #1 C.)Strain #4 D.)Strain #2 E.)Strain #3
In conjugation of a Hfr cell with an F- cell: a) the container cell becomes F + b) The container cell remains F- c) The donor cell becomes F- d) The recipient cell becomes Hfr
1a.The complete genotype of the host yeast strain is MATa ade2 his3 leu2 met15 ura3. Name one advantage of using a yeast strain with auxotrophies for several genes (i.e. his3, leu2 and ura3). b.)After successful CRIPSR editing, the yeast can later be “cured” of the recombinant CRISPR plasmid – that is, the plasmid is lost but the CRISPR edit is stably inherited. Write the new genotype of the yeast based on ADE6 gene.
As described in Figure, host DNA is hydrolyzed into smallpieces, which are occasionally assembled with phage proteins, creatinga phage with bacterial chromosomal DNA. If the breakage ofthe chromosomal DNA is not random (i.e., it is more likely tobreak at certain spots as opposed to other spots), how might nonrandombreakage affect cotransduction frequency?
Shown below are the maps of a series of rII− deletion strains (1–5). The deleted region is indicated as (......) and the intact region as ______. 1 ___________(...........)_______________ 2 _________________(...........)_________ 3 (.....................)_____________________ 4 ________________________(................) 5 _____(..........)______________________ rII− phage strains A-E have point mutations in the rII region. E.coli K(λ) cells are coinfected with one phage that has a deletion and one phage that has a point mutation. The presence of wild-type progeny phage is assessed by the presence (+) or absence (o) of plaques. 1 2 3 4 5 A + o + + + B o + + + + C + + + o + D + + o + + E + + o + o A) Indicate the order of the point mutations in the rII region. A) CADBE B) DEBAC C) BADCE D) ABDEC E) CEADB B) The test described here is a recombination test. Explain…
Arrange the following steps involved in Gene cloning in the correct orderi) Isolation of desired geneii) Insertion of isolated gene to the vectoriii) Introduction of recombinant vector to the hostiv)Expression of recombinant gene in hostv) Extraction of recombinant gene producta) i, ii, iii, iv, vb) v, iv, iii, ii, ic) i, iii, ii, iv, vd) iv, v, ii, i, iii
Define the following terms:a. transpositionb. transposable elementc. bacterial transformationd. transductione. conjugation
Conjugation depends on direct cell-to-cell contactbetween a donor F+ carrying either a conjugative________ (the F plasmid) or an integrated conjugativeelement (as in Hfr strains), and a recipient lacking suchan element (F−).

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