2. Write the equation involved in the hydrolysis of sucrose. HYDROLYSIS OF CARBOHYDRATESINTRODUCTION:Hydrolysis is the process where water is used to break long polysaccharidechains into smaller chains or into simple carbohydrates.Disaccharides can be hydrolyzed into two monosaccharaides units by the use oan enzyme or acid. Sucrose is table sugar which yields glucose and fructose whilelactose gives glucose and galactose after hydrolysis.Starch is a homopolysaccharide made up of d-glucose polymer. They arereserved carbohydrates of plants. They are made of two types of polymers namely:amylose (10-30%), no linear and has a 1,4 link and amylopectin (70-90%) is branchedhaving the a 1,4 link and a 1,6 link.Objectives: At the end of the activity, the student can:1. Hydrolyze the carbohydrate into its monosaccharide unit.2. Test the products of hydrolysis.Reagents: 6 ml Benedict's reagent, 3 ml Seliwanoff's reagent, 1 ml iodine in KI, 4 ml3M HCI, 4 ml 3M NaOH, 30 ml distilled water, 0.5 g starch, 0.5 g sucroseMaterials: 12(20 ml Test tube), 250 ml beaker, water bath, alcohol lamp, tripod,wire gauze, test tuberack, test tube brush, test tube holder, dropper,stirring rod, 10 ml graduated cylinder, platform balance.Procedure:STARCH - weigh 0.5 g starch and dissolve in 6 ml distilled water. Mix well.Divide the contents of the tube in 3 equal parts and label the tubes 1,2 and 3. Totube 1, add 2 ml 3M HCl; to tubes 2 and 3 two ml water each. Heat tubes 1 & 2in a boiling water bath for 30 minutes. Leave tube 3 at room temperature. After30 minutes, remove the tubes and cool. To tube 1, add 2 ml 3M NaOH and totubes 2& 3, two ml water each. Test each tube as follows:A.Benedict's test – place 1 ml benedict's reagent in 3 separate tube andlabel them tubes A, B, C. To tube A, add 5 drops of the solution in tube1, to tube B 5 drops of the solution from tube 2 and to tube C, 5 dropsfrom the solution in tube 3. Heat all 3 tubes in a boiling water bath for 2minutes. Compare the results of the 3 tubes.2. Iodine test – to the remaining solution in tubes 1, 2, 3, add 1 drop iodinein KI in each tube. Compare the results.B. SUCROSE - repeat the same procedure as in starch. Test each tube withbenedict's test but instead of performing the iodine test, do Seliwanoff's test.Seliwanoff'stest - add 1 ml Seliwanoff's reagent in 3 separate tube and labelA,B,C. To tube A, add 5 drops of the solution from tube 1, to tube B,,add 51drops of the solution from tube 2 and to tube C, 10 drops of the solution fromtube 3. Heat all tubes in a boiling water bath for 3 minutes. Compare the result.

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Carbohydrates are polymers composed of monosaccharide monomers. Carbohydrates play a variety of…