3.) A mixture of amino acids was subjected to paper chromatography with six amino acidstandards in order to determine its composition. After visualization using ninhydrin, themeasured distances were as follows:ABсDEFGa.) What is the Rf value of each amino acid observed?XDistances:Solvent 10.3cmA4.50cmB - 1.32cmC - 6.60cmD - 5.20cmE - 3.52cmF8.70cmG - 10.26cmX1 - 9.72cmX2 - 4.3cmX3 7.1cm-b.) What are the possible components of the amino acid mixture? Answer can be up to twoamino acids per component.

Paper chromatography is an in expensive powerful analytical technique to separate the solute…

Answered: 3.) A mixture of amino acids was…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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An amino acid mixture of phenylalanine, glycine and glutamic acid is to be separated by paper chromatography.The solvent is less polar than water. Which of these amino acids will have the highest Rf value and which the lowest? Explain.
An amino acid mixture of phenylalanine, glycine and glutamic acid is to be separated by paper chromatography. The solvent is less polar than water. Which of these amino acids will have the highest Rf value and which the lowest? Explain.
pH 3.5 4.5 5.5 6.5 7.5 8.5 Absorbance 0.098 0.027 0.068 0.028 0.032 0.054 Concentration in diluted supernatant (mg/ml) 0.196 0.054 0.136 0.0056 0.0064 0.0108 Concentration in undiluted supernatant (mg/ml) 0.98 0.27 0.68 0.28 0.32 0.54 Formula for the amount (g) of soluble proteins in the soy flour extract: In 15 ml of soy flour extract (with 1/50 dilution), Soluble protein (g) = (C x V x F) / 1000 C = concentration (mg/ml); F = dilution factor; V = volume of solution (ml) Calculate the % solubility of protein in the soy flour (Assume the soy flour contains 35% protein (w/w))
pH 3.5 4.5 5.5 6.5 7.5 8.5 Absorbance 0.098 0.027 0.068 0.028 0.032 0.054 Concentration in diluted supernatant (mg/ml) 0.196 0.054 0.136 0.0056 0.0064 0.0108 Concentration in undiluted supernatant (mg/ml) 0.98 0.27 0.68 0.28 0.32 0.54 Formula for the amount (g) of soluble proteins in the soy flour extract: In 15 ml of soy flour extract (with 1/50 dilution), Soluble protein (g) = (C x V x F) / 1000 C = concentration (mg/ml); F = dilution factor; V = volume of solution (ml) Calculate the amount of soluble protein (in grams) in the soy flour extract
Assuming equal concentrations of conjugate base and acid, which one of the following mixtures is suitable for making a buffer solution with an optimum pH of 7.4–7.6? NaOCl / HOCl (K a = 3.2 × 10 –8) NH 3 / NH 4Cl (K a = 5.6 × 10 –10) NaNO 2 / HNO 2 (K a = 4.5 × 10 –4) NaCl / HCl CH 3COONa / CH 3COOH (K a = 1.8 × 10 –5)
Explain how you would prepare 100ml of 10mMProline solution given that the molecular mass of proline= 115.13 g/mol
If I have a 20ml stock solution of BSA with a BSA concentration of 0.2%. How can I make two other 20ml solutions with 97% and 75% the concentration of the stock solution, using the stock solution and two other graduated cylinders?..
3. a) An aqueous solution is 3.50% by mass potassium bromide, KBr, and has a density of 1.02 g/mL. The molality of potassium bromide in the solution is _________m. b)An aqueous solution of magnesium nitrate has a concentration of 0.474 molal. The percent by mass of magnesium nitrate in the solution is _________%. c)An aqueous solution of cobalt(II) bromide has a concentration of 0.153 molal. The percent by mass of cobalt(II) bromide in the solution is _________%.
Table 2. Volume of BSA, protein content, and absorbance readings of reference solutions Solution Volume of BSA standard solution (μL) Protein content(μg/mL) Absorbance value At 595 nm 1 0 0 0 2 10 1 0.022 3 30 3 0.065 4 50 5 0.106 5 70 7 0.178 6 100 10 0.299 7 120 12 0.380 Make a graph by plotting the absorbance values versus the BSA protein content (in μg) for theseven reference solutions. When constructing the graph, be…
Provide a brief definition and a description of the significance of the following terms in NMR spectroscopy (4) a 90o pulse(5) J coupling(6) distance geometry
The pH probe/meter uses following equations: Ecell = L + 0.0592 log a1 = L - 0.0592 pH Where L = L1 + EAg/AgCI + Easy= constants L1 = - 0.0592 log a2 a1 = activity of analyte solution a2 = activity of internal solution How will measured pH value be affected vs “real” pH if the temperature of the sample is 30C when pH was measured ? a.measured pH is lower than real pH b.impact can not be determined c.measured pH is higher than real pH d.measured pH is same as real pH
The pH probe/meter uses following equations: Ecell = L + 0.0592 log a1 = L - 0.0592 pH Where L = L1 + EAg/AgCI + Easy= constants L1 = - 0.0592 log a2 a1 = activity of analyte solution a2 = activity of internal solution How will measured pH value be affected vs “real” pH if HCl in pH electrode, became 0.15M instead of 0.1M ? a.impact can not be determined b.measured pH is higher than "real" pH. c.measured pH is lower than "real" pH. d.measured pH is same as "real" pH.

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3.) A mixture of amino acids was subjected to paper chromatography with six amino acid standards in order to determine its composition. After visualization using ninhydrin, the measured distances were as follows: