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- https://www.youtube.com/watch?v=rKng5-ij6kQ Provide a schematic diagram for the Molish test methodologies in determining the presence of carbohydrates. Also, give the basic principle for the test. (not a graded question, the video is very brief) (please take time to answer)Answer the following questions (not more than 5 sentences/question). Discuss the correct laboratory technique in using the centrifuge for qualitative analysis. Give two tips on its proper use. Explain how calcium ions are confirmed present in a solid salt by using flame test. Discuss a method to confirm the presence of a) phenol and b) ketone in a test compound. How are proteins confirmed present in a sample? Explain the laboratory process.Match the objective of the test. -Mucic acid Test -Seliwanoff's Test -Bial's Test -Nylander's Test -Barfoed's Test Detects optical activity of reducing sugar Confirms the presence of pentose Differentiates reducing monosaccharide from reducing disaccharide Detects presence of glucose in urine Confirms presence of polyhydroxyaldehyde Identifies the presence of C=O in ketohexose Detects presence of brain sugar General test for polymers of polyhydroxy aldehyde and ketones
- Procedure: 1. Prepare 5 test tubes. Place 1 ml of 1% starch and add 10 drops of saliva to each tube. Mix thoroughly. 2. Place the first tube in ice water, the 2nd tube leave at room temperature, the 3rd tube in 40°C , the 4th tube at 60°Cwater bath and the 5th tube boil for 2 minutes.. 3. Leave the 4 tubes in their respective temperatures for 30 minutes. The 4th tube allows to stand for 30 minutes after heating for 2 minutes. 4. Test the contents of each tube with iodine and benedict’s tests.Suggest how the weak points of the Molisch test for carbohydrates can be strengthened by comparing it to other carbohydrate testsWhich of the different carbohydrate tests would give a positive result for maltose? Describe the color change. Molisch test Fehling’s test Benedict’s test Tollen’s Test Barfoed’s test Seliwanoff’s test Phenylhydrazine or osazone test
- Complete the table below. Indicate what is observed if each test is positive. State what this result implies about each of the carbohydrates tested. TEST EXPECTED OBSERVATION FOR A POSITIVE TEST SUBSTANCE INDICATED BY A POSITIVE TEST I2 Solution Molisch Barfoed Seliwanoff Bial- OrcinolIn a tabular form, enumerate other chemical qualitative tests for carbohydrates, discuss its principle, chemical reagents employed and its positive test result.Explain the chemical basis (reaction) of Millon’s test. (Generic reaction and explanation) What is the difference between Millon's test and Biuret test?(minimum 5)
- What is the phenol red test? Please explain the testing and its results and the chemistry behind it? Please explain for a beginners level. Thank youBy applying Gravimetry, Titration (Chemical indicator) and Potentiometry lessons which technique should be used in following situations. Explain your answer. In your explanation, state what is the advantage of the technique you chose over other technique and /or the barrier/difficulty of using the technique. Your choice should not be dependent on cost. For chem students- Total Acid content per tablet of a newly developed drug that has yellow coating Target level is 1%.Based on this video https://www.youtube.com/watch?v=rKng5-ij6kQ Provide a schematic diagram for the Molish ’s test methodologies in determining the presence of carbohydrates. Also, give the basic principle for the test.