2. What microorganisms other than coliforms are likely to give positive presumptive test?
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2. What microorganisms other than coliforms are likely to give positive presumptive test?
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- 1. The selective ingredient in mannitol salt agar is ______________, which selects for the growth of Staphylococcus organisms.2. Please explain how the use of an Eosin Methylene Blue Agar plate can help determine the type of fermenters bacteria are1. The colonies on a positive MSA plate would appear ________________. 2. Which of the following tests can be used to distinguish between Coliform and Non-coliform strains? Mannitol Salt agar Coagulase test McConkey agar Bile eschulin agar Simmon's Citrate agar
- 1. Explain why the following steps are essential during sub-culturing: a. Flaming the inoculating instrument prior to and after each inoculation b. Holding the test tube caps in the hand as illustrated in Figure 1. c. Cooling the inoculating instrument prior to obtaining the inoculum d. Flaming the neck of the tubes immediately after uncapping and before recapping 2. Describe the purposes of the sub-culture procedure. 3. Explain why a straight inoculating needle is used to inoculate an agar deep tube. 4. What is the indication of bacterial growth in each of the media? (nutrient broth, nutrient agar slant, nutrient agar deep? 5. Enumerate the Good Microbiological Practices encountered in the activity. 6. Upon observation of the nutrient agar slant culture, you strongly suspect that the culture is contaminated. Outline the method you would follow to ascertain whether your suspicion is justified.2. Mechanical disinfection is typically more effective than manual techniques.3. Why not test water samples directly for Salmonella typhosa or other pathogens?
- 1. How would you interpret the following result of the Bile Esculin Test: "Growth of the bacteria is present however no darkening of the agar can be observed after 48 hours." Please explain.1. What are the two ways that nitrate can be reduced by the bacteria. Explain briefly. 2. What organisms are being differentiated in the butyrate disc test. Explain its reaction to the test.1-The method of sterilizations we used in the tissue culture lab a. Fumigation b. Filtration C. Autoclave d. Sun light
- 1. “Coliform bacteria are the only group of micro-organisms considered suitable for themonitoring of faecal pollution.”Is the statement above still valid? Motivate your answer and briefly describe at two othertypes of indicator organisms. 2.4. discuss the disinfection protocols for removing potential pathogens in frequently- used environments.1. Determine the amount of dehydrated medium needed to prepare 50 nutrient agar plates. Include amount for 2 additional plates as excess to compensate for compounding losses. 2.