8. You have completed an in-vitro mutagenesis experiment to create an A →I mutation inthe first codon of the actin gene. This gene is present on a plasmid and you want toconfirm that the mutation was correctly introduced. Which sequencing technology be mostappropriate to use and why? Please limit your response to a single paragraph. 7. What aspects of the filament-polymerization process are common to actin andmicrotubules? What features are different? (Please limit your answer to bullet points or asingle paragraph).

A gene is a sequence of DNAs. DNA is composed of nucleotide sequences. During the process of…

Answered: 8. You have completed an in-vitro…

Biology 2e

2nd Edition

ISBN:9781947172517

Author:Matthew Douglas, Jung Choi, Mary Ann Clark

Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark

Chapter4: Cell Structure

Section: Chapter Questions

Problem 20RQ: Which of the following do not play a role in intracellular movement? microfilaments and intermediate...

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Similar questions

Which of the following do not play a role in intracellular movement? microfilaments and intermediate filaments microfilaments and microtubules intermediate filaments and microtubules only intermediate filaments
1.What are the functions of the following structures? Nebulin: Troponin: Tropomyosin: Draw and label these components on the thin filament.What is the physical relationship between the 2 types of filaments?
Actin and microtubules have polarity, intermediate filaments have no polarity. What is the main consequence of this difference?
1.Cytochalasin is a drug that caps actin filament plus ends, thus preventing actin polymerization. Phalloidin is a drug that binds to and stabilizes actin filaments, preventing actin depolymerization. Even though these drugs have opposite effects on actin polymerization, the addition of either of these drugs instantaneously freezes the cell movements that depend on actin filaments. Explain why drugs that have opposite effects on actin filaments can have a similar effect on cell movements.
You’re having a discussion with a colleague who insists that intermediate filaments, IFs, provide very little mechanical strength to cells. How can you rebut this and provide quantitative, experimental evidence to measure the strength of an intermediate filament using microscopy?
Microfilaments are long filamentous proteins made up of many globular proteins. What main protein are microfilaments composed of?
3) Collagen is a very important structural protein in animals. Discuss the various parts of the body in which collagen is an important structural molecule and what its function is at each body location.
Actin filaments, microtubules, and bacterial flagella are all built from small subunits. Describe three advantages of assembling long filamentous structures from subunits rather than from single, long proteins.
Neurofilament proteins assemble into long, intermediate filaments , found in abundance running along the length of nerve cell axons. The C-terminal region of these proteins is an unstructured polypeptide, hundreds of amino acids long and heavily modified by the addition of phosphate groups. The term “polymer brush” has been applied to this part of the neurofilament. can you suggest why?
2. Name one protein that is part of the sliding filament theory
1. When considering tight junctions, adherens junctions, and desmosomes: a. Which ones do not help in resisting mechanical stress and holding cell shape? Why not? b. Considering how the mechanical junctions are placed in the plasma membrane, why don’t they provide a truly sealed barrier?
There are no known motor proteins that move along intermediate filaments, in contrast to the variety of such proteins that associate with microtubules and actin filaments. What characteristic of intermediate filaments, in their polymerized form, might explain the absence of associated motor proteins? Briefly explain your reasoning.

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