A 50.0 ml juice extract is colorimetrically assayed using Nelson's test. One milliliter (1.00 mL) of the solution and the standard glucose solution (concentration: Img/mL) were treated with freshly prepared Nelson's reagent and arsenomolybdate reagent and then diluted to 10.0 mL separately in properly labeled test tubes. Absorbance at 480 nm for the standard is 1.702 and for the sample, 0.926. A. What is the principle behind the assay?
A 50.0 ml juice extract is colorimetrically assayed using Nelson's test. One milliliter (1.00 mL) of the solution and the standard glucose solution (concentration: Img/mL) were treated with freshly prepared Nelson's reagent and arsenomolybdate reagent and then diluted to 10.0 mL separately in properly labeled test tubes. Absorbance at 480 nm for the standard is 1.702 and for the sample, 0.926. A. What is the principle behind the assay?
Chapter12: Ratio And Proportion
Section: Chapter Questions
Problem 6.5P
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Transcribed Image Text:III. Problem solving. Show your computations. Answer questions briefly.
A 50.0 ml juice extract is colorimetrically assayed using Nelson's test. One
milliliter (1.00 mL) of the solution and the standard glucose solution (concentration:
1mg/mL) were treated with freshly prepared Nelson's reagent and arsenomolybdate
reagent and then diluted to 10.0 mL separately in properly labeled test tubes.
Absorbance at 480 nm for the standard is 1.702 and for the sample, 0.926.
A. What is the principle behind the assay?
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