A chromatogram of a two-component mixture on a 25-cm packed LC column is shown in the figure below. The flow rate was 0.40 mL/min. a. Find the times that components A and B spend in the stationary phase. b. Find the retention times for A and B. c. Determine the retention factors for the two components. d. Find the full widths of each peak. e. Find the resolution of the two peaks. f. Find the average number of plates for the column g. Find the average plate height. h. What column length would be needed to achieve a resolution of 1.75? 16 A 14 12 B 0. 0. 10 30 40 50 60 70 Time, min 20 2. 10 Detector response

A chromatogram of a two-component mixture on a 25-cm packed LC column is shown in the figure below. The flow rate was 0.40 mL/min. a. Find the times that components A and B spend in the stationary phase. b. Find the retention times for A and B. c. Determine the retention factors for the two components. d. Find the full widths of each peak. e. Find the resolution of the two peaks. f. Find the average number of plates for the column g. Find the average plate height. h. What column length would be needed to achieve a resolution of 1.75? 16 A 14 12 B 0. 0. 10 30 40 50 60 70 Time, min 20 2. 10 Detector response

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter27: Gas Chromatography

Section: Chapter Questions

Problem 27.22QAP

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