What is the result of applying too much sample to the TLC plate? Select one: Some of the sample moves down instead of up Reduced separation between the spots O The sample moves past the solvent front O The spot does not leave the origin O The sample leeches out into the elution solvent
Q: A student spots an unknown sample on a TLC plate and develops it in pentane solvent. Only one spot,…
A: The retardation factor (Rf) provides the interaction of the substance with the thin layer of the…
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Q: Fresh solvent - eluent Mobile phase Sample components A Celumn packing Stationary phase suspended in…
A: B is less polar and A is more polar Because the stationary phase is polar, polar molecules will…
Q: What happens when the level of the eluent solution in the developing chamber (beaker) is adjusted to…
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Q: Which of the following factors DOES NOT affect retention time of a sample undergoing normal phase…
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Q: economical purification technique that ca n be pure material? O Distillation O Another extraction…
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Q: Retention time Substance(s) Peak area (%) (min) Crude product Pure product 8.725 1,2-cyclohexanediol…
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Q: Which components in a liquid chromatography (LC) instrument are similar to the experimental set-up?
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Q: Is the separation of the mixture shown here a physical orchemical process?
A: SOLUTION: Step 1: The separation of the mixture shown above (i.e. by column chromatography) is a…
Q: C. >) Order the compound A, B and C from lowest to highest Rr. The sample is spotted on a silica gel…
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Q: What is wrong with the following TLC setup? Developing solvent Origin
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Q: order to execute paper chromatography, which of the following stages is required? I. Load the…
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Q: Before developing a TLC plate you should _______. a. Make sure to mark the origin with a pen b.…
A: While developing a TLC plate, we should always use a pencil (not pen) because: Pencils are cheap and…
Q: Which one of the following approaches would be inappropriate for the introduction of the sample in…
A: ICP-MS is a type of mass spectroscopy which is used to ionize a sample.
Q: 2. A TIc plate was run on the fractions (#1-4) collected from a silica gravity column chromatography…
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Q: Thin Layer Chromatography why the non-polar compounds move up the plate most rapidly (higher Rf…
A: TLC is most useful technique to know the extent of the reaction .And the no of compound present in…
Q: Demonstrate how to make TLC, and analyze the sample for any purpose, which deemed suitable for you
A: TLC is a thin layer chromatography, a technique used for separating non-volatile mixtures and…
Q: 2. A Tlc plate was run on the fractions (#1-4) collected from a silica gravity column chromatography…
A: According to the company guidelines, we have to answer the first question only. So, please kindly…
Q: In reverse phase chromatography which analyte will elute first, most polar or least polar?
A: In reverse phase chromatography, polar solvent is used as a mobile phase.
Q: 24 45 11 4 16 19 21 12 15 3 6 8 9 10 23 13 14 17 18 20 22 1 2 6. 8. 10 12 14 16 18 20 22 24 26 Time…
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Q: Explain how solvent trapping and cold trapping work in splitless injection.
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Q: TLC
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Q: Calculate the Rf value for a spot in a TLC experiment if the solvent moved 14.5 cm and the spot…
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Q: Vhat criteria should you use to determine the solvent for dissolving a sample for TLC analysis? Vhat…
A: the solvent should be 2-3 mm deep so that the spotted sample and the line are not submerged this…
Q: Which substance is best to run the column chromatography? a)The solvent that is very polar B)The…
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Q: Give 2 examples of High performance liquid chromatography (HPLC) on how or when it is recommended to…
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Q: For the TLCS shown below, both plates have the exact same materials spotted on each lane (that is,…
A: Thin layer chromatography.
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Q: What are the main differences in column size, operating pressure, sample volume and run time between…
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Q: How is the retention factor calculated for a specific spot when doing the TLC analysis? O Distance…
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Q: A solute with a retention time of 400.0 s has a width at half-height of 8.0 s on a column 12.2 m…
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Q: 1. State the reason why we need to compare the observed retention time values to the standard values…
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Q: When would you choose to run a TLC? Choose all that apply. to determine if a sample is pure to…
A: Thin layer chromatography (TLC) is a separation technique using a small quantity of the sample.
Q: A mixture of species of A, B, and C is injected into a liquid chromatographic column with length of…
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Q: What will be the appearance of a TLC plate if a solvent of too low polarity is used for the…
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Q: What is the retention factor of a sample when after injectiion the void time (tM) took 4.18 sec? The…
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Q: At the completion of the experiment, a student measures the solvent front to be 55 mm from the…
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Q: For the TLCS shown below, both plates have the exact same materials spotted on each lane (that is,…
A: Distance Travelled by the Sample depends upon the type of Polar mobile phase used . IF THE mobile…
Q: From the experiment in Question 1, the following data were obtained: 2. Distance traveled by spot O…
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Q: Calculate the Rf value for a spot in a TLC experiment if the solvent moved 13.5 cm and the spot…
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Q: A chromatogram of a two-component mixture on a 25-cm packed LC column is shown in the figure below.…
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Q: What are the R f values of the spots on this TLC plate? Solvent front |7.9 7.2 8.4 1.6 1.4 Baseline…
A: The expression for the Rf value is shown below: Rf = distance travelled by spotdistance travelled by…
Q: Question 5 Which part of the HPLC contains majority of the solvent? O fraction collector O reservoir…
A: The beginning of the HPLC system occurs with the solvent reservoir which contains the solvent , and…
Q: (7) Once you put the spotted TLC plate into the solvent, you will see the solvent begin to move up…
A: Since you have asked multiple questions, we will answer first one for you. For remaining questions,…
Q: The following conditions may not cause tailing EXCEPT a. Thick filter paper as the stationary phase…
A: Tailing in thin layer chromatography : Due to the using high concentration of solute or other…
Q: How column is developed and solvent selection is done in HPLC?
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Q: Rf of spot A in the TLC plate below is: Solvent front A 10 cm OB 8 cm 6 cm 2 cm Origin Select one: O…
A: The Rf value of a component depends on the distance travelled by the component and the distance…
Q: A TLC plate showed two spots A and B with Rf 0.20 and 0.30, respectively. Sketch a TLC chromatogram…
A: Sketch of TLC chromatogram: TLC plate = 10cm
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- Can you DRAW a TLC plate in an elution chamber that shows 3 lanes each with one spot. Label the following in your drawing: Solvent front Origin Mobile phase Stationary phase Distances needed to measure Rf value for one of the spotsThe solvent level in the development chamber should be above the level of the spotted compounds on the TLC plate. Group of answer choices True FalseA student spots an unknown sample on a TLC plate and develops it in dichloromethane solvent. Only one spot, for which the Rf value is 0.95, is observed. Does this indicate that the unknown material is a pure compound? What can be done to verify the purity of the sample?
- Where should the analyte be spotted on a TLC plate? Below the 1 cm spot line On the 1 cm spot line. On the solvent front line Above the 1 cm spot lineIn order to achieve the best separation of several components of different polarity in a mixture spotted on a TLC plate, what guideline should one follow regarding the choice of a developing solvent? (i.e. polar, non-polar, etc,)A TLC plate showed two spots A and B with Rf 0.20 and 0.30, respectively. Sketch a TLC chromatogram with the above Rf values. (Hint: Start with a 10-cm TLC plate and allow 1 cm each for the start and end solvent fronts.) Determine the distances travelled from the start of the solvent front. ___?____ A ____?___ B Determine the distances left to the end of the solvent front. ___?____ A ____?___ B
- How does increasing the polarity of the developing solvent affect the Rf of a compound on a silica gel TLC plate?A student spots an unknown sample on a TLC plate and develops it in pentane solvent. Only one spot, for which the Rf value is 0.05, is observed. Is the un-known material a pure compound? What can be done to verify the purity of the sample?You spotted a mixture of three compounds, labeled A-C, on a silica plate. The resulting thin layer chromatography (TLC) plate with a mobile solvent mixture of 95 % hexanes and 5 % ethyl acetate is shown below. Which of the spots traveled the furthest up the TLC plate corresponding to spot 3?
- Two compounds A and B from GC on two open tubular columns contains a XY-1 (100% methyl) and a XY-2 (50% methyl, 50% phenyl), with the same diameter, thickness and length under the same experimental conditions. The retention times and peak widths are show in the Table 1.Hint: phenyl groups interact more strongly with polar groups than methyl groups. Which compound is more polar? Explain your answerExplain why it is needed to filter the solvent before introducing it to the HPLC systemIf a column was run on the sample shown in lane 3, what would be the first compound to elute off of the column? (assume the same solvent and stationary phase are used) Which of the lanes on this TLC plate show pure compounds? Select all that apply.