A lab scale gel chromatography column packed with Superdex 200 is used to separate two antibodies A and B. The column is 10 cm in diameter and 30 cm long. The void volume of the column is 1.8 x 10 m². The water regain value of the gel is 3 × 10³ m² kg and the density of the wetted gel is 1.21 x 10¹ kg m³. The partition coefficients for antibodies A and B are 0.17 and the 0.42 respectively. If the eluent flow rate is 650 mL h, calculate the retention time for each antibody.
A lab scale gel chromatography column packed with Superdex 200 is used to separate two antibodies A and B. The column is 10 cm in diameter and 30 cm long. The void volume of the column is 1.8 x 10 m². The water regain value of the gel is 3 × 10³ m² kg and the density of the wetted gel is 1.21 x 10¹ kg m³. The partition coefficients for antibodies A and B are 0.17 and the 0.42 respectively. If the eluent flow rate is 650 mL h, calculate the retention time for each antibody.
Chapter10: Reconstitution Of Powdered Drugs
Section: Chapter Questions
Problem 7.2P
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Transcribed Image Text:(b) A lab scale gel chromatography column packed with Superdex 200 is used to separate
Vtwo antibodies A and B. The column is 10 cm in diameter and 30 cm long. The void
volume of the column is 1.8 x 10
m³. The water regain value of the gel is 3 × 10³ m²
kg and the density of the wetted gel is 1.21 x 10³ kg m³. The partition coefficients for
antibodies A and B are 0.17 and the 0.42 respectively. If the eluent flow rate is 650 mL
h¹, calculate the retention time for each antibody.
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